The protective effects of dietary resistant starch against post-antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosis
Compromised bone quality increases the risk of fractures in domesticate birds, resulting in pain and altered behaviour. Although dietary resistant starch (RS) supplementation show promise for improving inferior bone mass, the diet-mediated gut microbiota alterations as a potential mechanism underlyi...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125004808 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850069303388274688 |
|---|---|
| author | Y.Y. Huang S.M. Qin M.T. Nguyen W. Chen X.M. Si Y.Q. Huang H.Y. Zhang |
| author_facet | Y.Y. Huang S.M. Qin M.T. Nguyen W. Chen X.M. Si Y.Q. Huang H.Y. Zhang |
| author_sort | Y.Y. Huang |
| collection | DOAJ |
| description | Compromised bone quality increases the risk of fractures in domesticate birds, resulting in pain and altered behaviour. Although dietary resistant starch (RS) supplementation show promise for improving inferior bone mass, the diet-mediated gut microbiota alterations as a potential mechanism underlying RS positive roles in bone remains uncertain. With a post-antibiotic model and faecal microbiota transplantation (FMT), this study investigated the effects of a RS diet on antibiotic-induced bone loss and gut microbial composition in meat ducks. Ducklings were assigned to 4 treatments with 6 replicate pens until 21 d, including the control group (Ctrl, feeding a basal diet) and the RS-fed group, and post-antibiotic treatment following the gavage of phosphate-buffered saline (Post-anti-PBS) or faecal microbiota transplantation (Post-anti-FMT). The RS diet increased the proportion of Firmicutes, improved intestinal integrity, and reduced inflammation-induced bone resorption, all of which contributed to an increase in tibial bone volume (P < 0.05). Post-antibiotic treatment was found to reduce tibial quality by stimulating bone resorption and inhibiting bone formation, accompanied by gut microbiota dysbiosis, increased intestinal permeability (P = 0.059), and inflammatory flare compared to control birds. FMT from RS-fed ducks into the antibiotic-treated birds reversed bone loss by primarily blocking osteoclastic frequency and activity. Furthermore, FMT increased the ratio of Firmicutes to Bacteroidetes (P < 0.05) and suppressed the release of pro-osteoclastogenic cytokines such as tumour necrosis factor-α (P = 0.062) and interleukin-1β (P < 0.05) in the bone marrow. These results demonstrated the involvement of gut microbiota in improving bone quality of meat ducks by RS, and FMT of RS-fed birds corrected the imbalance of ceca microbiota and attenuated bone loss in meat ducks with enhanced bone resorption. |
| format | Article |
| id | doaj-art-1c75a9d5fe9c4ad8af0dbc43e9e54b85 |
| institution | DOAJ |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-1c75a9d5fe9c4ad8af0dbc43e9e54b852025-08-20T02:47:49ZengElsevierPoultry Science0032-57912025-08-01104810523810.1016/j.psj.2025.105238The protective effects of dietary resistant starch against post-antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosisY.Y. Huang0S.M. Qin1M.T. Nguyen2W. Chen3X.M. Si4Y.Q. Huang5H.Y. Zhang6College of Animal Science and Technology, Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450046, PR ChinaCollege of Animal Science and Technology, Southwest University, Chongqing 402460, PR ChinaAgriculture and Forestry, Hue University, Hue 49000, VietnamCollege of Animal Science and Technology, Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450046, PR ChinaCollege of Animal Science and Technology, Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450046, PR ChinaCollege of Animal Science and Technology, Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450046, PR ChinaCollege of Animal Science and Technology, Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450046, PR China; Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Sciences and Aquatic Ecology, Ghent University, Ghent 9000, Belgium; Corresponding author.Compromised bone quality increases the risk of fractures in domesticate birds, resulting in pain and altered behaviour. Although dietary resistant starch (RS) supplementation show promise for improving inferior bone mass, the diet-mediated gut microbiota alterations as a potential mechanism underlying RS positive roles in bone remains uncertain. With a post-antibiotic model and faecal microbiota transplantation (FMT), this study investigated the effects of a RS diet on antibiotic-induced bone loss and gut microbial composition in meat ducks. Ducklings were assigned to 4 treatments with 6 replicate pens until 21 d, including the control group (Ctrl, feeding a basal diet) and the RS-fed group, and post-antibiotic treatment following the gavage of phosphate-buffered saline (Post-anti-PBS) or faecal microbiota transplantation (Post-anti-FMT). The RS diet increased the proportion of Firmicutes, improved intestinal integrity, and reduced inflammation-induced bone resorption, all of which contributed to an increase in tibial bone volume (P < 0.05). Post-antibiotic treatment was found to reduce tibial quality by stimulating bone resorption and inhibiting bone formation, accompanied by gut microbiota dysbiosis, increased intestinal permeability (P = 0.059), and inflammatory flare compared to control birds. FMT from RS-fed ducks into the antibiotic-treated birds reversed bone loss by primarily blocking osteoclastic frequency and activity. Furthermore, FMT increased the ratio of Firmicutes to Bacteroidetes (P < 0.05) and suppressed the release of pro-osteoclastogenic cytokines such as tumour necrosis factor-α (P = 0.062) and interleukin-1β (P < 0.05) in the bone marrow. These results demonstrated the involvement of gut microbiota in improving bone quality of meat ducks by RS, and FMT of RS-fed birds corrected the imbalance of ceca microbiota and attenuated bone loss in meat ducks with enhanced bone resorption.http://www.sciencedirect.com/science/article/pii/S0032579125004808Resistant starchFaecal microbiota transplantationBone metabolismGut microbiotaMeat duck |
| spellingShingle | Y.Y. Huang S.M. Qin M.T. Nguyen W. Chen X.M. Si Y.Q. Huang H.Y. Zhang The protective effects of dietary resistant starch against post-antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosis Poultry Science Resistant starch Faecal microbiota transplantation Bone metabolism Gut microbiota Meat duck |
| title | The protective effects of dietary resistant starch against post-antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosis |
| title_full | The protective effects of dietary resistant starch against post-antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosis |
| title_fullStr | The protective effects of dietary resistant starch against post-antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosis |
| title_full_unstemmed | The protective effects of dietary resistant starch against post-antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosis |
| title_short | The protective effects of dietary resistant starch against post-antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosis |
| title_sort | protective effects of dietary resistant starch against post antibiotic bone loss in meat ducks associated with the recovery of caecal microbiota dysbiosis |
| topic | Resistant starch Faecal microbiota transplantation Bone metabolism Gut microbiota Meat duck |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125004808 |
| work_keys_str_mv | AT yyhuang theprotectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT smqin theprotectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT mtnguyen theprotectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT wchen theprotectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT xmsi theprotectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT yqhuang theprotectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT hyzhang theprotectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT yyhuang protectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT smqin protectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT mtnguyen protectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT wchen protectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT xmsi protectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT yqhuang protectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis AT hyzhang protectiveeffectsofdietaryresistantstarchagainstpostantibioticbonelossinmeatducksassociatedwiththerecoveryofcaecalmicrobiotadysbiosis |