Melia azedarach L. (Meliaceae): Phytochemical Analysis and Evaluation of Antibacterial Activity of Fruit Extracts.

Background Medicinal plants are still the main source of therapeutic substances for treating infectious diseases that seriously endanger human health in South Africa. The current study examined the potential therapeutic applications of the young, ripe, and mature fruits of M. azedarach. Method...

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Main Authors: Phakamani Linda Masuku, Himansu Baijnath, Karishma Singh
Format: Article
Language:English
Published: Student's Journal of Health Research 2025-03-01
Series:Student's Journal of Health Research Africa
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Online Access:https://sjhresearchafrica.org/index.php/public-html/article/view/1616
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author Phakamani Linda Masuku
Himansu Baijnath
Karishma Singh
author_facet Phakamani Linda Masuku
Himansu Baijnath
Karishma Singh
author_sort Phakamani Linda Masuku
collection DOAJ
description Background Medicinal plants are still the main source of therapeutic substances for treating infectious diseases that seriously endanger human health in South Africa. The current study examined the potential therapeutic applications of the young, ripe, and mature fruits of M. azedarach. Methods A standard protocol, which included chemical reagents and a series of reactions, was used to determine the presence of the phytochemical compound classes. The methanol and hexane extract of young, ripe, and mature fruits were applied to six bacterial strains (Methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus) to evaluate their antibacterial activity. Results Methanol extracts of young, ripe, and mature fruits tested positive for six bioactive compounds. Hexane extracts of young, ripe, and mature fruits tested positive for four bioactive compounds. All six bacterial strains were highly susceptible to the methanol extract of fruits. Klebsiella pneumoniae and P. aeruginosa were strongly resistant to hexane extracts of the young fruits. Klebsiella pneumoniae, E. coli, MRSA, and P. aeruginosa were strongly resistant to hexane extracts of the ripe fruits. Klebsiella pneumonia and E. coli were strongly resistant to hexane extracts of the mature fruits. Conclusion Melia azedarach fruits, whether young, ripe, or mature, contain bioactive therapeutic compounds (Carbohydrates, Amino acids, Alkaloids, Flavonoids, Saponins, Sterols, Steroids/Terpenoids, Phenols, Mucilage and Gums, Fixed oils, and fats) that can be used to develop medicines to treat various human ailments and display strong antibacterial potential. Recommendations Future research is needed to evaluate each bioactive compound's antibacterial activity and efficacy to determine which can be used as components in producing antibacterial medicines and drugs.
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spelling doaj-art-1c72e9a328dd41f6b0b8bb4f578c4fa02025-08-20T02:42:11ZengStudent's Journal of Health ResearchStudent's Journal of Health Research Africa2709-99972025-03-0163110https://doi.org/10.51168/sjhrafrica.v6i3.1616Melia azedarach L. (Meliaceae): Phytochemical Analysis and Evaluation of Antibacterial Activity of Fruit Extracts. Phakamani Linda Masuku 0Himansu Baijnath 1Karishma Singh 2School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000, South Africa. School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000, South Africa. Department of Nature Conservation, Faculty of Natural Sciences, Mangosuthu University of Technology, P.O. Box 12363, Jacobs, 4026, Durban, KwaZulu- Natal, South Africa Background Medicinal plants are still the main source of therapeutic substances for treating infectious diseases that seriously endanger human health in South Africa. The current study examined the potential therapeutic applications of the young, ripe, and mature fruits of M. azedarach. Methods A standard protocol, which included chemical reagents and a series of reactions, was used to determine the presence of the phytochemical compound classes. The methanol and hexane extract of young, ripe, and mature fruits were applied to six bacterial strains (Methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus) to evaluate their antibacterial activity. Results Methanol extracts of young, ripe, and mature fruits tested positive for six bioactive compounds. Hexane extracts of young, ripe, and mature fruits tested positive for four bioactive compounds. All six bacterial strains were highly susceptible to the methanol extract of fruits. Klebsiella pneumoniae and P. aeruginosa were strongly resistant to hexane extracts of the young fruits. Klebsiella pneumoniae, E. coli, MRSA, and P. aeruginosa were strongly resistant to hexane extracts of the ripe fruits. Klebsiella pneumonia and E. coli were strongly resistant to hexane extracts of the mature fruits. Conclusion Melia azedarach fruits, whether young, ripe, or mature, contain bioactive therapeutic compounds (Carbohydrates, Amino acids, Alkaloids, Flavonoids, Saponins, Sterols, Steroids/Terpenoids, Phenols, Mucilage and Gums, Fixed oils, and fats) that can be used to develop medicines to treat various human ailments and display strong antibacterial potential. Recommendations Future research is needed to evaluate each bioactive compound's antibacterial activity and efficacy to determine which can be used as components in producing antibacterial medicines and drugs.https://sjhresearchafrica.org/index.php/public-html/article/view/1616melia azedarachbioactive compoundsantibacterial activityinhibition zoneresistance
spellingShingle Phakamani Linda Masuku
Himansu Baijnath
Karishma Singh
Melia azedarach L. (Meliaceae): Phytochemical Analysis and Evaluation of Antibacterial Activity of Fruit Extracts.
Student's Journal of Health Research Africa
melia azedarach
bioactive compounds
antibacterial activity
inhibition zone
resistance
title Melia azedarach L. (Meliaceae): Phytochemical Analysis and Evaluation of Antibacterial Activity of Fruit Extracts.
title_full Melia azedarach L. (Meliaceae): Phytochemical Analysis and Evaluation of Antibacterial Activity of Fruit Extracts.
title_fullStr Melia azedarach L. (Meliaceae): Phytochemical Analysis and Evaluation of Antibacterial Activity of Fruit Extracts.
title_full_unstemmed Melia azedarach L. (Meliaceae): Phytochemical Analysis and Evaluation of Antibacterial Activity of Fruit Extracts.
title_short Melia azedarach L. (Meliaceae): Phytochemical Analysis and Evaluation of Antibacterial Activity of Fruit Extracts.
title_sort melia azedarach l meliaceae phytochemical analysis and evaluation of antibacterial activity of fruit extracts
topic melia azedarach
bioactive compounds
antibacterial activity
inhibition zone
resistance
url https://sjhresearchafrica.org/index.php/public-html/article/view/1616
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AT karishmasingh meliaazedarachlmeliaceaephytochemicalanalysisandevaluationofantibacterialactivityoffruitextracts