Ecotin protects Salmonella Typhimurium against the microbicidal activity of host proteases.
Salmonella enterica serovar Typhimurium causes acute diarrhea upon oral infection in humans. The harsh and proteolytic environment found in the gastrointestinal tract is the first obstacle that these bacteria face after infection. However, the mechanisms that allow Salmonella to survive the hostile...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-03-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1013013 |
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| author | Lucas Saposnik Lorena M Coria Laura Bruno Francisco F Guaimas Julieta Pandolfi Melina Pol Maria Eugenia Urga Florencia Sabbione Michael McClelland Analia Trevani Karina A Pasquevich Juliana Cassataro |
| author_facet | Lucas Saposnik Lorena M Coria Laura Bruno Francisco F Guaimas Julieta Pandolfi Melina Pol Maria Eugenia Urga Florencia Sabbione Michael McClelland Analia Trevani Karina A Pasquevich Juliana Cassataro |
| author_sort | Lucas Saposnik |
| collection | DOAJ |
| description | Salmonella enterica serovar Typhimurium causes acute diarrhea upon oral infection in humans. The harsh and proteolytic environment found in the gastrointestinal tract is the first obstacle that these bacteria face after infection. However, the mechanisms that allow Salmonella to survive the hostile conditions of the gut are poorly understood. The ecotin gene is found in an extensive range of known phyla of bacteria and it encodes a protein that has been shown to inhibit serine proteases. Thus, in the present work we studied the role of ecotin of Salmonella Typhimurium in host-pathogen interactions. We found that the Salmonella Typhimurium ∆ecotin strain exhibited lower inflammation in a murine model of Salmonella induced colitis. The ∆ecotin mutant was more susceptible to the action of pancreatin and purified pancreatic elastase. In addition, the lack of ecotin led to impaired adhesion to Caco-2 and HT-29 cell lines, related to the proteolytic activity of brush border enzymes. Besides, ∆ecotin showed higher susceptibility to lysosomal proteolytic content and intracellular replication defects in macrophages. In addition, we found Ecotin to have a crucial role in Salmonella against the microbicidal action of granule contents and neutrophil extracellular traps released from human polymorphonuclear leukocytes. Thus, the work presented here highlights the importance of ecotin in Salmonella as countermeasures against the host proteolytic defense system. |
| format | Article |
| id | doaj-art-1c48e34c4d214c80b9d2dd28a65fe515 |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-1c48e34c4d214c80b9d2dd28a65fe5152025-08-20T02:16:09ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742025-03-01213e101301310.1371/journal.ppat.1013013Ecotin protects Salmonella Typhimurium against the microbicidal activity of host proteases.Lucas SaposnikLorena M CoriaLaura BrunoFrancisco F GuaimasJulieta PandolfiMelina PolMaria Eugenia UrgaFlorencia SabbioneMichael McClellandAnalia TrevaniKarina A PasquevichJuliana CassataroSalmonella enterica serovar Typhimurium causes acute diarrhea upon oral infection in humans. The harsh and proteolytic environment found in the gastrointestinal tract is the first obstacle that these bacteria face after infection. However, the mechanisms that allow Salmonella to survive the hostile conditions of the gut are poorly understood. The ecotin gene is found in an extensive range of known phyla of bacteria and it encodes a protein that has been shown to inhibit serine proteases. Thus, in the present work we studied the role of ecotin of Salmonella Typhimurium in host-pathogen interactions. We found that the Salmonella Typhimurium ∆ecotin strain exhibited lower inflammation in a murine model of Salmonella induced colitis. The ∆ecotin mutant was more susceptible to the action of pancreatin and purified pancreatic elastase. In addition, the lack of ecotin led to impaired adhesion to Caco-2 and HT-29 cell lines, related to the proteolytic activity of brush border enzymes. Besides, ∆ecotin showed higher susceptibility to lysosomal proteolytic content and intracellular replication defects in macrophages. In addition, we found Ecotin to have a crucial role in Salmonella against the microbicidal action of granule contents and neutrophil extracellular traps released from human polymorphonuclear leukocytes. Thus, the work presented here highlights the importance of ecotin in Salmonella as countermeasures against the host proteolytic defense system.https://doi.org/10.1371/journal.ppat.1013013 |
| spellingShingle | Lucas Saposnik Lorena M Coria Laura Bruno Francisco F Guaimas Julieta Pandolfi Melina Pol Maria Eugenia Urga Florencia Sabbione Michael McClelland Analia Trevani Karina A Pasquevich Juliana Cassataro Ecotin protects Salmonella Typhimurium against the microbicidal activity of host proteases. PLoS Pathogens |
| title | Ecotin protects Salmonella Typhimurium against the microbicidal activity of host proteases. |
| title_full | Ecotin protects Salmonella Typhimurium against the microbicidal activity of host proteases. |
| title_fullStr | Ecotin protects Salmonella Typhimurium against the microbicidal activity of host proteases. |
| title_full_unstemmed | Ecotin protects Salmonella Typhimurium against the microbicidal activity of host proteases. |
| title_short | Ecotin protects Salmonella Typhimurium against the microbicidal activity of host proteases. |
| title_sort | ecotin protects salmonella typhimurium against the microbicidal activity of host proteases |
| url | https://doi.org/10.1371/journal.ppat.1013013 |
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