Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen
Objective. To quantify Enterococcus faecalis density in root canal dentin after chemomechanical preparation (CMP) using alternated irrigating regimen. Methodology. Root canals (RC) were contaminated with E. faecalis (ATCC 19433) for 3 weeks and evident biofilms were obtained. After initial sampling...
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| Language: | English |
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Wiley
2019-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2019/9219238 |
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| author | Janir Alves Soares Maria Auxiliadora Roque de Carvalho Suelleng Maria Cunha Santos Soares Rodrigo Dantas Pereira Manoel Brito-Júnior Paula Prazeres Magalhães Isabel Alessandra Miranda Nunes Rudys Rodolpho de Jesus Tavarez Claudia de Castro Rizzi Rafaela Nogueira Moreira Luiz de Macêdo Farias Etevaldo Matos Maia Filho |
| author_facet | Janir Alves Soares Maria Auxiliadora Roque de Carvalho Suelleng Maria Cunha Santos Soares Rodrigo Dantas Pereira Manoel Brito-Júnior Paula Prazeres Magalhães Isabel Alessandra Miranda Nunes Rudys Rodolpho de Jesus Tavarez Claudia de Castro Rizzi Rafaela Nogueira Moreira Luiz de Macêdo Farias Etevaldo Matos Maia Filho |
| author_sort | Janir Alves Soares |
| collection | DOAJ |
| description | Objective. To quantify Enterococcus faecalis density in root canal dentin after chemomechanical preparation (CMP) using alternated irrigating regimen. Methodology. Root canals (RC) were contaminated with E. faecalis (ATCC 19433) for 3 weeks and evident biofilms were obtained. After initial sampling (S1), the CMP was aided by irrigants: saline solution (control; n=12), a conventional regimen (CR) (group 1; n=12) using 5.25% NaOCl and a final rinse with 17% EDTA, and an alternating regimen (AR) of intercalated use of NaOCl and EDTA (group 2, n=12), followed by a second sampling (S2). After 2 weeks, S3 was obtained. Two roots were analyzed by scanning electron microscopy. Each root was divided into cervical, mild, and apical segments and sampling of the superficial (n=90) and deep (n=90) dentin layers was obtained using Gates-Glidden burs. The E. faecalis density (CFU/mg) in log10 was categorized as residual (0 > 0.2), moderate (0.2 ≥ 0.5), or elevated (> 0.5). The prevalence of positive samples in BHI and BHI-A was analyzed by Pearson’s chi-square test. The data were normalized by a log10 transformation of CFU and were analyzed by one-way ANOVA and Tukey’s tests. Results. Biofilms were observed only in the control root canal walls. Topographically, the controls and CR showed similar distributions of E. faecalis in the dentin. Microbiologically positive root canals harbored much E. faecalis in the adjacent dentin (p < 0.05). Irrigating saline provided moderate density of E. faecalis in the dentin while CR and AR resulted in a residual density of microorganisms (p < 0.05). Conclusions. The Enterococcus faecalis density in dentin was influenced by the irrigating regimen and the microbiological status of the root canal. The CMP aided by the alternating regimen interfered with the recolonization of the root canal and topographic distribution of Enterococcus in root dentin. |
| format | Article |
| id | doaj-art-1c4838039fcc49cab25d9b81f76c6e5c |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-1c4838039fcc49cab25d9b81f76c6e5c2025-02-03T01:03:11ZengWileyThe Scientific World Journal2356-61401537-744X2019-01-01201910.1155/2019/92192389219238Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating RegimenJanir Alves Soares0Maria Auxiliadora Roque de Carvalho1Suelleng Maria Cunha Santos Soares2Rodrigo Dantas Pereira3Manoel Brito-Júnior4Paula Prazeres Magalhães5Isabel Alessandra Miranda Nunes6Rudys Rodolpho de Jesus Tavarez7Claudia de Castro Rizzi8Rafaela Nogueira Moreira9Luiz de Macêdo Farias10Etevaldo Matos Maia Filho11Department of Dentistry, Federal University of the Jequitinhonha and Mucuri Valleys, Diamantina, Minas Gerais, BrazilDepartment of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Dentistry, Federal University of the Jequitinhonha and Mucuri Valleys, Diamantina, Minas Gerais, BrazilDepartment of Dentistry, State University of Montes Claros, Montes Claros, Minas Gerais, BrazilDepartment of Dentistry, State University of Montes Claros, Montes Claros, Minas Gerais, BrazilDepartment of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Dentistry, Ceuma University, São Luís, Maranhão, BrazilDepartment of Dentistry, Ceuma University, São Luís, Maranhão, BrazilDepartment of Dentistry, Ceuma University, São Luís, Maranhão, BrazilDepartment of Dentistry, Federal University of the Jequitinhonha and Mucuri Valleys, Diamantina, Minas Gerais, BrazilDepartment of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Dentistry, Ceuma University, São Luís, Maranhão, BrazilObjective. To quantify Enterococcus faecalis density in root canal dentin after chemomechanical preparation (CMP) using alternated irrigating regimen. Methodology. Root canals (RC) were contaminated with E. faecalis (ATCC 19433) for 3 weeks and evident biofilms were obtained. After initial sampling (S1), the CMP was aided by irrigants: saline solution (control; n=12), a conventional regimen (CR) (group 1; n=12) using 5.25% NaOCl and a final rinse with 17% EDTA, and an alternating regimen (AR) of intercalated use of NaOCl and EDTA (group 2, n=12), followed by a second sampling (S2). After 2 weeks, S3 was obtained. Two roots were analyzed by scanning electron microscopy. Each root was divided into cervical, mild, and apical segments and sampling of the superficial (n=90) and deep (n=90) dentin layers was obtained using Gates-Glidden burs. The E. faecalis density (CFU/mg) in log10 was categorized as residual (0 > 0.2), moderate (0.2 ≥ 0.5), or elevated (> 0.5). The prevalence of positive samples in BHI and BHI-A was analyzed by Pearson’s chi-square test. The data were normalized by a log10 transformation of CFU and were analyzed by one-way ANOVA and Tukey’s tests. Results. Biofilms were observed only in the control root canal walls. Topographically, the controls and CR showed similar distributions of E. faecalis in the dentin. Microbiologically positive root canals harbored much E. faecalis in the adjacent dentin (p < 0.05). Irrigating saline provided moderate density of E. faecalis in the dentin while CR and AR resulted in a residual density of microorganisms (p < 0.05). Conclusions. The Enterococcus faecalis density in dentin was influenced by the irrigating regimen and the microbiological status of the root canal. The CMP aided by the alternating regimen interfered with the recolonization of the root canal and topographic distribution of Enterococcus in root dentin.http://dx.doi.org/10.1155/2019/9219238 |
| spellingShingle | Janir Alves Soares Maria Auxiliadora Roque de Carvalho Suelleng Maria Cunha Santos Soares Rodrigo Dantas Pereira Manoel Brito-Júnior Paula Prazeres Magalhães Isabel Alessandra Miranda Nunes Rudys Rodolpho de Jesus Tavarez Claudia de Castro Rizzi Rafaela Nogueira Moreira Luiz de Macêdo Farias Etevaldo Matos Maia Filho Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen The Scientific World Journal |
| title | Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen |
| title_full | Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen |
| title_fullStr | Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen |
| title_full_unstemmed | Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen |
| title_short | Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen |
| title_sort | pattern of disinfection of root canal dentin by alternated acid base irrigating regimen |
| url | http://dx.doi.org/10.1155/2019/9219238 |
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