Specific Inhibition of PCR by Non-Extendable Oligonucleotides Using a 5′ to 3′ Exonuclease-Deficient DNA Polymerase
The Stoffel fragment of Taq DNA polymerase lacks the 5′to 3′exonuclease activity that hydrolyzes potentially blocking DNA strands during primer extension. We therefore asked whether by using this fragment in the PCR, non-extendable, base-paired oligonucleotides could inhibit amplification in a seque...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97234st06 |
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