The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (<i>Apis mellifera</i>)

High temperature is normally harmful to an organism. However, honey bees evolve, maintaining a relatively higher colony temperature of 34.5 °C in the long reproduction period. To determine the effect of such a higher colony temperature on adult bees and its biological significance, newly emerged bee...

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Main Authors: Chaoxia Sun, Hongji Huang, Mei Yang, Guoshuai Ma, Xinyao Huang, Shaokang Huang, Xinle Duan, Jianghong Li
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Insects
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Online Access:https://www.mdpi.com/2075-4450/16/6/558
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author Chaoxia Sun
Hongji Huang
Mei Yang
Guoshuai Ma
Xinyao Huang
Shaokang Huang
Xinle Duan
Jianghong Li
author_facet Chaoxia Sun
Hongji Huang
Mei Yang
Guoshuai Ma
Xinyao Huang
Shaokang Huang
Xinle Duan
Jianghong Li
author_sort Chaoxia Sun
collection DOAJ
description High temperature is normally harmful to an organism. However, honey bees evolve, maintaining a relatively higher colony temperature of 34.5 °C in the long reproduction period. To determine the effect of such a higher colony temperature on adult bees and its biological significance, newly emerged bees were reared in cages at 34.5 °C and room temperature of 25.0 °C, respectively. Their survival rate, head weight, royal jelly-secreting gene expression, and morphology of the hypopharyngeal gland were investigated. Moreover, 40-day-old bees with significant differences in survival rate between the two temperature groups were subject to transcriptome and lipidome analysis. The result showed that the higher colony temperature was overall negative for the bees’ longevity. Transcriptome analysis showed that fatty acid metabolism-related items were enriched and the involved genes were upregulated in honey bees reared at 34.5 °C compared with the honey bees reared at 25.0 °C. Lipidomic analysis further validated that fatty acid metabolism, especially sphingolipid metabolism, was significantly altered. Such upregulation of fatty acid metabolism-related genes was also detected in young adult bees of 5 days old reared at 34.5 °C. These bees had heavier head weights, higher expression of royal jelly-secreting-related genes, and more developed hypopharyngeal glands. Such results showed that the colony temperature of 34.5 °C could accelerate the development process of newly emerged bees to be nurse bees, significantly increasing the colony nurturing capability, which in turn increased the development speed, size, and survivability of the colony. Thereby, the colony temperature of 34.5 °C shortened the lifespan of individual bees, but obtained huge returns at the colony level, with remarkable biological significance.
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spelling doaj-art-1c346243ae4b4d05bd814d7766d8c6e22025-08-20T03:27:14ZengMDPI AGInsects2075-44502025-05-0116655810.3390/insects16060558The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (<i>Apis mellifera</i>)Chaoxia Sun0Hongji Huang1Mei Yang2Guoshuai Ma3Xinyao Huang4Shaokang Huang5Xinle Duan6Jianghong Li7College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaHigh temperature is normally harmful to an organism. However, honey bees evolve, maintaining a relatively higher colony temperature of 34.5 °C in the long reproduction period. To determine the effect of such a higher colony temperature on adult bees and its biological significance, newly emerged bees were reared in cages at 34.5 °C and room temperature of 25.0 °C, respectively. Their survival rate, head weight, royal jelly-secreting gene expression, and morphology of the hypopharyngeal gland were investigated. Moreover, 40-day-old bees with significant differences in survival rate between the two temperature groups were subject to transcriptome and lipidome analysis. The result showed that the higher colony temperature was overall negative for the bees’ longevity. Transcriptome analysis showed that fatty acid metabolism-related items were enriched and the involved genes were upregulated in honey bees reared at 34.5 °C compared with the honey bees reared at 25.0 °C. Lipidomic analysis further validated that fatty acid metabolism, especially sphingolipid metabolism, was significantly altered. Such upregulation of fatty acid metabolism-related genes was also detected in young adult bees of 5 days old reared at 34.5 °C. These bees had heavier head weights, higher expression of royal jelly-secreting-related genes, and more developed hypopharyngeal glands. Such results showed that the colony temperature of 34.5 °C could accelerate the development process of newly emerged bees to be nurse bees, significantly increasing the colony nurturing capability, which in turn increased the development speed, size, and survivability of the colony. Thereby, the colony temperature of 34.5 °C shortened the lifespan of individual bees, but obtained huge returns at the colony level, with remarkable biological significance.https://www.mdpi.com/2075-4450/16/6/558colony temperatureadult beeslongevitynurturing capabilitylipid metabolism
spellingShingle Chaoxia Sun
Hongji Huang
Mei Yang
Guoshuai Ma
Xinyao Huang
Shaokang Huang
Xinle Duan
Jianghong Li
The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (<i>Apis mellifera</i>)
Insects
colony temperature
adult bees
longevity
nurturing capability
lipid metabolism
title The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (<i>Apis mellifera</i>)
title_full The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (<i>Apis mellifera</i>)
title_fullStr The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (<i>Apis mellifera</i>)
title_full_unstemmed The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (<i>Apis mellifera</i>)
title_short The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (<i>Apis mellifera</i>)
title_sort trade off between the increased colony nurturing ability and the decreased lifespan of worker bees i apis mellifera i
topic colony temperature
adult bees
longevity
nurturing capability
lipid metabolism
url https://www.mdpi.com/2075-4450/16/6/558
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