Effects of cancer-induced cachexia and administration of l-glutathione on the intestinal mucosa in rat
Abstract Walker-256 tumor is an experimental model known to promote cachexia syndrome, oxidative stress, and systemic inflammation. This study evaluated the duodenal mucosa of rats with Walker-256 tumor administered with 1% l-glutathione, intending to evaluate the damage caused by cancer-associated...
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| Format: | Article |
| Language: | English |
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Springer
2024-04-01
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| Series: | Amino Acids |
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| Online Access: | https://doi.org/10.1007/s00726-024-03391-9 |
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| author | Sabrina Silva Sestak Fabiana Galvão da Motta Lima Ana Paula de Oliveira Letícia Ganem Rillo Paz Barateiro Flávia Cristina Vieira-Frez Sara Raquel Garcia de Souza Flávia Alessandra Guarnier Juliana Vanessa Colombo Martins Perles Jacqueline Nelisis Zanoni |
| author_facet | Sabrina Silva Sestak Fabiana Galvão da Motta Lima Ana Paula de Oliveira Letícia Ganem Rillo Paz Barateiro Flávia Cristina Vieira-Frez Sara Raquel Garcia de Souza Flávia Alessandra Guarnier Juliana Vanessa Colombo Martins Perles Jacqueline Nelisis Zanoni |
| author_sort | Sabrina Silva Sestak |
| collection | DOAJ |
| description | Abstract Walker-256 tumor is an experimental model known to promote cachexia syndrome, oxidative stress, and systemic inflammation. This study evaluated the duodenal mucosa of rats with Walker-256 tumor administered with 1% l-glutathione, intending to evaluate the damage caused by cancer-associated cachexia in the gastrointestinal tract and the effects of antioxidant administration on mucosal protection. Twenty-four 55-day-old male Wistar rats were distributed into four groups: control (C); control administered with 1% l-glutathione (C-GSH); Walker-256 tumor (W) and Walker-256 tumor administered with 1% l-glutathione (W-GSH). After 14 days of treatment, the duodenum was harvested for morphometric analysis of the mucosa, proliferation, apoptosis, immunostaining of varicosities immunoreactive (IR) to vasoactive intestinal peptide (VIP) and 5-HT-IR cells, and quantification of mast cells and goblet cells. Walker-256 tumor-bearing rats showed cachexia syndrome, mucosal atrophy, reduced cell proliferation, reduced 5-HT-IR cells, and increased goblet cells and VIPergic varicosities, which were not reversed by l-glutathione. On the other hand, l-glutathione caused a reduction of cells in apoptosis and mast cell recruitment, demonstrating a partial recovery of the damage detected in the intestinal mucosa. |
| format | Article |
| id | doaj-art-1c31dfe196bd463e8519d4f3ca86da23 |
| institution | DOAJ |
| issn | 1438-2199 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Springer |
| record_format | Article |
| series | Amino Acids |
| spelling | doaj-art-1c31dfe196bd463e8519d4f3ca86da232025-08-20T02:39:41ZengSpringerAmino Acids1438-21992024-04-0156111410.1007/s00726-024-03391-9Effects of cancer-induced cachexia and administration of l-glutathione on the intestinal mucosa in ratSabrina Silva Sestak0Fabiana Galvão da Motta Lima1Ana Paula de Oliveira2Letícia Ganem Rillo Paz Barateiro3Flávia Cristina Vieira-Frez4Sara Raquel Garcia de Souza5Flávia Alessandra Guarnier6Juliana Vanessa Colombo Martins Perles7Jacqueline Nelisis Zanoni8Department of Physiology, Laboratory of Enteric Neural Plasticity, State University of Maringá, O33 BlockDepartment of Physiology, Laboratory of Enteric Neural Plasticity, State University of Maringá, O33 BlockDepartment of Physiology, Laboratory of Enteric Neural Plasticity, State University of Maringá, O33 BlockDepartment of Morphological Sciences, State University of MaringáDepartment of Morphological Sciences, State University of MaringáDepartment of Morphological Sciences, State University of MaringáDepartment of Pathology Sciences, State University of LondrinaDepartment of Morphological Sciences, State University of MaringáDepartment of Physiology, Laboratory of Enteric Neural Plasticity, State University of Maringá, O33 BlockAbstract Walker-256 tumor is an experimental model known to promote cachexia syndrome, oxidative stress, and systemic inflammation. This study evaluated the duodenal mucosa of rats with Walker-256 tumor administered with 1% l-glutathione, intending to evaluate the damage caused by cancer-associated cachexia in the gastrointestinal tract and the effects of antioxidant administration on mucosal protection. Twenty-four 55-day-old male Wistar rats were distributed into four groups: control (C); control administered with 1% l-glutathione (C-GSH); Walker-256 tumor (W) and Walker-256 tumor administered with 1% l-glutathione (W-GSH). After 14 days of treatment, the duodenum was harvested for morphometric analysis of the mucosa, proliferation, apoptosis, immunostaining of varicosities immunoreactive (IR) to vasoactive intestinal peptide (VIP) and 5-HT-IR cells, and quantification of mast cells and goblet cells. Walker-256 tumor-bearing rats showed cachexia syndrome, mucosal atrophy, reduced cell proliferation, reduced 5-HT-IR cells, and increased goblet cells and VIPergic varicosities, which were not reversed by l-glutathione. On the other hand, l-glutathione caused a reduction of cells in apoptosis and mast cell recruitment, demonstrating a partial recovery of the damage detected in the intestinal mucosa.https://doi.org/10.1007/s00726-024-03391-9AntioxidantDuodenumOxidative stressWalker-256 tumor |
| spellingShingle | Sabrina Silva Sestak Fabiana Galvão da Motta Lima Ana Paula de Oliveira Letícia Ganem Rillo Paz Barateiro Flávia Cristina Vieira-Frez Sara Raquel Garcia de Souza Flávia Alessandra Guarnier Juliana Vanessa Colombo Martins Perles Jacqueline Nelisis Zanoni Effects of cancer-induced cachexia and administration of l-glutathione on the intestinal mucosa in rat Amino Acids Antioxidant Duodenum Oxidative stress Walker-256 tumor |
| title | Effects of cancer-induced cachexia and administration of l-glutathione on the intestinal mucosa in rat |
| title_full | Effects of cancer-induced cachexia and administration of l-glutathione on the intestinal mucosa in rat |
| title_fullStr | Effects of cancer-induced cachexia and administration of l-glutathione on the intestinal mucosa in rat |
| title_full_unstemmed | Effects of cancer-induced cachexia and administration of l-glutathione on the intestinal mucosa in rat |
| title_short | Effects of cancer-induced cachexia and administration of l-glutathione on the intestinal mucosa in rat |
| title_sort | effects of cancer induced cachexia and administration of l glutathione on the intestinal mucosa in rat |
| topic | Antioxidant Duodenum Oxidative stress Walker-256 tumor |
| url | https://doi.org/10.1007/s00726-024-03391-9 |
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