Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis—A Comparative Study
Mastitis is one of the most frequent diseases in dairy farms and occurs in both clinical and subclinical forms, resulting in substantial economic losses. Asymmetrical dimethylarginine (ADMA) and symmetrical dimethylarginine (SDMA) are biomarkers that inhibit nitric oxide synthesis. Elevated ADMA lev...
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2025-02-01
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| author | Valerio Bronzo Giulia Sala Irene Ciabattini Chiara Orsetti Giovani Armenia Valentina Meucci Lucia De Marchi Fabrizio Bertelloni Micaela Sgorbini Francesca Bonelli |
| author_facet | Valerio Bronzo Giulia Sala Irene Ciabattini Chiara Orsetti Giovani Armenia Valentina Meucci Lucia De Marchi Fabrizio Bertelloni Micaela Sgorbini Francesca Bonelli |
| author_sort | Valerio Bronzo |
| collection | DOAJ |
| description | Mastitis is one of the most frequent diseases in dairy farms and occurs in both clinical and subclinical forms, resulting in substantial economic losses. Asymmetrical dimethylarginine (ADMA) and symmetrical dimethylarginine (SDMA) are biomarkers that inhibit nitric oxide synthesis. Elevated ADMA levels are associated with an increased risk of mortality both in human medicine and in dogs and a potential need for intensive care, while SDMA correlates with poor prognoses in humans and the progression of renal disease in horses, though its impact varies depending on renal function. This study examines the plasma levels of ADMA and SDMA in healthy cows (H) and cows with subclinical mastitis (SCM) and clinical mastitis (CM). Cows were classified as having mastitis when CMT > 1 and SCC ≥ 250,000 cells/mL. The SCM group showed no clinical signs or milk alterations, whereas the CM group exhibited udder and/or milk changes. The study included 196 blood samples to determine ADMA and SDMA concentrations, with 96 from healthy cows and 100 from pathological cows (58 SCM and 42 CM). The descriptive statistics were reported as the median because the data were not normally distributed (Shapiro–Wilk test). Data were analyzed using the Kruskal–Wallis test with Bonferroni post hoc correction, and the cut-off and accuracy index were calculated using the gold-standard measurement, the SCC. Statistically significant differences in ADMA levels were observed between healthy cows (0.11 µmol/L) and cows with mastitis (SCM 0.26 µmol/L; CM 0.26 µmol/L), but no differences were found in their SDMA levels. The cut-off for ADMA was >0.164 µmol/L, with a sensitivity of 80.41% and specificity of 77.78%. This study suggests that the blood concentration of ADMA is statistically higher in cows with subclinical and clinical mastitis and could be further explored as a potential biomarker for diagnosing these diseases. |
| format | Article |
| id | doaj-art-1c2aa72f3eca4eb6ba175b9173ceee8b |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-1c2aa72f3eca4eb6ba175b9173ceee8b2025-08-20T02:01:20ZengMDPI AGAnimals2076-26152025-02-0115452710.3390/ani15040527Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis—A Comparative StudyValerio Bronzo0Giulia Sala1Irene Ciabattini2Chiara Orsetti3Giovani Armenia4Valentina Meucci5Lucia De Marchi6Fabrizio Bertelloni7Micaela Sgorbini8Francesca Bonelli9Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyMastitis is one of the most frequent diseases in dairy farms and occurs in both clinical and subclinical forms, resulting in substantial economic losses. Asymmetrical dimethylarginine (ADMA) and symmetrical dimethylarginine (SDMA) are biomarkers that inhibit nitric oxide synthesis. Elevated ADMA levels are associated with an increased risk of mortality both in human medicine and in dogs and a potential need for intensive care, while SDMA correlates with poor prognoses in humans and the progression of renal disease in horses, though its impact varies depending on renal function. This study examines the plasma levels of ADMA and SDMA in healthy cows (H) and cows with subclinical mastitis (SCM) and clinical mastitis (CM). Cows were classified as having mastitis when CMT > 1 and SCC ≥ 250,000 cells/mL. The SCM group showed no clinical signs or milk alterations, whereas the CM group exhibited udder and/or milk changes. The study included 196 blood samples to determine ADMA and SDMA concentrations, with 96 from healthy cows and 100 from pathological cows (58 SCM and 42 CM). The descriptive statistics were reported as the median because the data were not normally distributed (Shapiro–Wilk test). Data were analyzed using the Kruskal–Wallis test with Bonferroni post hoc correction, and the cut-off and accuracy index were calculated using the gold-standard measurement, the SCC. Statistically significant differences in ADMA levels were observed between healthy cows (0.11 µmol/L) and cows with mastitis (SCM 0.26 µmol/L; CM 0.26 µmol/L), but no differences were found in their SDMA levels. The cut-off for ADMA was >0.164 µmol/L, with a sensitivity of 80.41% and specificity of 77.78%. This study suggests that the blood concentration of ADMA is statistically higher in cows with subclinical and clinical mastitis and could be further explored as a potential biomarker for diagnosing these diseases.https://www.mdpi.com/2076-2615/15/4/527dairy cowudder healthbiomarkerADMASDMA |
| spellingShingle | Valerio Bronzo Giulia Sala Irene Ciabattini Chiara Orsetti Giovani Armenia Valentina Meucci Lucia De Marchi Fabrizio Bertelloni Micaela Sgorbini Francesca Bonelli Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis—A Comparative Study Animals dairy cow udder health biomarker ADMA SDMA |
| title | Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis—A Comparative Study |
| title_full | Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis—A Comparative Study |
| title_fullStr | Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis—A Comparative Study |
| title_full_unstemmed | Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis—A Comparative Study |
| title_short | Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis—A Comparative Study |
| title_sort | endogenous symmetric dimethylarginine sdma and asymmetrical dimethylarginine adma levels in healthy cows and cows with subclinical and clinical mastitis a comparative study |
| topic | dairy cow udder health biomarker ADMA SDMA |
| url | https://www.mdpi.com/2076-2615/15/4/527 |
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