In silico construction of polyvalent multi-epitope respiratory syncytial virus subunit vaccine: focusing on prevalent HLA allele types in Korea

Respiratory syncytial virus (RSV) is a leading cause of lower respiratory infections in infants and the elderly. The burden of RSV-related hospitalizations and deaths is significantly increasing. Recently, RSV vaccines targeting F protein have been approved, but their long-term efficacy is yet to be...

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Main Authors: Myoung Hee Han, Bomi Yoon, Min-Kyu Kim, Seonghee Park, Jae Hyang Lim
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Taibah University for Science
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Online Access:https://www.tandfonline.com/doi/10.1080/16583655.2025.2514284
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author Myoung Hee Han
Bomi Yoon
Min-Kyu Kim
Seonghee Park
Jae Hyang Lim
author_facet Myoung Hee Han
Bomi Yoon
Min-Kyu Kim
Seonghee Park
Jae Hyang Lim
author_sort Myoung Hee Han
collection DOAJ
description Respiratory syncytial virus (RSV) is a leading cause of lower respiratory infections in infants and the elderly. The burden of RSV-related hospitalizations and deaths is significantly increasing. Recently, RSV vaccines targeting F protein have been approved, but their long-term efficacy is yet to be evaluated. This study aimed to design a polyvalent multi-epitope RSV subunit vaccine tailored to Korean HLA types using in silico methods. Nine prevalent HLA allele types in Korea were used to predict vaccine epitopes. Combinations of selected epitopes were created, and stable combinations were selected and conjugated with human β-defensin and PADRE. Fifty-five multi-epitope combinations were selected for vaccine construction, and 33 vaccine constructs induced strong immune responses in silico. The final vaccine candidate is a 228-amino acid construct weighing 23,957 Da and effectively induces humoral and cellular immune responses with high population coverage, suggesting potential for further development.
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series Journal of Taibah University for Science
spelling doaj-art-1c2a24c478b146d5bd1fe00d6477f0022025-08-20T02:05:32ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552025-12-0119110.1080/16583655.2025.2514284In silico construction of polyvalent multi-epitope respiratory syncytial virus subunit vaccine: focusing on prevalent HLA allele types in KoreaMyoung Hee Han0Bomi Yoon1Min-Kyu Kim2Seonghee Park3Jae Hyang Lim4Department of Microbiology, College of Medicine, Ewha Womans University, Seoul, Republic of KoreaDepartment of Microbiology, College of Medicine, Ewha Womans University, Seoul, Republic of KoreaAdvanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of KoreaDepartment of Physiology, College of Medicine, Ewha Womans University, Seoul, Republic of KoreaDepartment of Microbiology, College of Medicine, Ewha Womans University, Seoul, Republic of KoreaRespiratory syncytial virus (RSV) is a leading cause of lower respiratory infections in infants and the elderly. The burden of RSV-related hospitalizations and deaths is significantly increasing. Recently, RSV vaccines targeting F protein have been approved, but their long-term efficacy is yet to be evaluated. This study aimed to design a polyvalent multi-epitope RSV subunit vaccine tailored to Korean HLA types using in silico methods. Nine prevalent HLA allele types in Korea were used to predict vaccine epitopes. Combinations of selected epitopes were created, and stable combinations were selected and conjugated with human β-defensin and PADRE. Fifty-five multi-epitope combinations were selected for vaccine construction, and 33 vaccine constructs induced strong immune responses in silico. The final vaccine candidate is a 228-amino acid construct weighing 23,957 Da and effectively induces humoral and cellular immune responses with high population coverage, suggesting potential for further development.https://www.tandfonline.com/doi/10.1080/16583655.2025.2514284Respiratory syncytial virusmultiepitopein silicorecombinant subunit vaccine
spellingShingle Myoung Hee Han
Bomi Yoon
Min-Kyu Kim
Seonghee Park
Jae Hyang Lim
In silico construction of polyvalent multi-epitope respiratory syncytial virus subunit vaccine: focusing on prevalent HLA allele types in Korea
Journal of Taibah University for Science
Respiratory syncytial virus
multiepitope
in silico
recombinant subunit vaccine
title In silico construction of polyvalent multi-epitope respiratory syncytial virus subunit vaccine: focusing on prevalent HLA allele types in Korea
title_full In silico construction of polyvalent multi-epitope respiratory syncytial virus subunit vaccine: focusing on prevalent HLA allele types in Korea
title_fullStr In silico construction of polyvalent multi-epitope respiratory syncytial virus subunit vaccine: focusing on prevalent HLA allele types in Korea
title_full_unstemmed In silico construction of polyvalent multi-epitope respiratory syncytial virus subunit vaccine: focusing on prevalent HLA allele types in Korea
title_short In silico construction of polyvalent multi-epitope respiratory syncytial virus subunit vaccine: focusing on prevalent HLA allele types in Korea
title_sort in silico construction of polyvalent multi epitope respiratory syncytial virus subunit vaccine focusing on prevalent hla allele types in korea
topic Respiratory syncytial virus
multiepitope
in silico
recombinant subunit vaccine
url https://www.tandfonline.com/doi/10.1080/16583655.2025.2514284
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