BAFF promotes Th17 cells and aggravates experimental autoimmune encephalomyelitis.
<h4>Background</h4>BAFF, in addition to promoting B cell survival and differentiation, may affect T cells. The objective of this study was to determine the effect of BAFF on Th17 cell generation and its ramifications for the Th17 cell-driven disease, EAE.<h4>Methodology/principal f...
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023629&type=printable |
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| author | Xiaohui Zhou Zanxian Xia Qin Lan Julie Wang Wenru Su Yuan-Ping Han Huimin Fan Zhongmin Liu William Stohl Song Guo Zheng |
| author_facet | Xiaohui Zhou Zanxian Xia Qin Lan Julie Wang Wenru Su Yuan-Ping Han Huimin Fan Zhongmin Liu William Stohl Song Guo Zheng |
| author_sort | Xiaohui Zhou |
| collection | DOAJ |
| description | <h4>Background</h4>BAFF, in addition to promoting B cell survival and differentiation, may affect T cells. The objective of this study was to determine the effect of BAFF on Th17 cell generation and its ramifications for the Th17 cell-driven disease, EAE.<h4>Methodology/principal findings</h4>Th17 cells were increased in BAFF-Tg B6 (B6.BTg) mice and decreased in B6.Baff(-/-) mice. Th17 cells in B6.Baff(-/-) mice bearing a BAFF Tg (B6.Baff(-/-).BTg mice) were identical to those in B6.BTg mice, indicating that membrane BAFF is dispensable for Th17 cell generation as long as soluble BAFF is plentiful. In T + non-T cell criss-cross co-cultures, Th17 cell generation was greatest in cultures containing B6.BTg T cells and lowest in cultures containing B6.Baff(-/-) T cells, regardless of the source of non-T cells. In cultures containing only T cells, Th17 cell generation followed an identical pattern. CD4(+) cell expression of CD126 (IL-6R α chain) was increased in B6.BTg mice and decreased in B6.Baff(-/-) mice, and activation of STAT3 following stimulation with IL-6 + TGF-β was also greatest in B6.BTg cells and lowest in B6.Baff(-/-) cells. EAE was clinically and pathologically most severe in B6.BTg mice and least severe in B6.Baff(-/-) mice and correlated with MOG(35-55) peptide-induced Th17 cell responses.<h4>Conclusions/significance</h4>Collectively, these findings document a contribution of BAFF to pathogenic Th17 cell responses and suggest that BAFF antagonism may be efficacious in Th17 cell-driven diseases. |
| format | Article |
| id | doaj-art-1c14bde876fc49a98ed86a1e6302ecd0 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-1c14bde876fc49a98ed86a1e6302ecd02025-08-20T03:09:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2362910.1371/journal.pone.0023629BAFF promotes Th17 cells and aggravates experimental autoimmune encephalomyelitis.Xiaohui ZhouZanxian XiaQin LanJulie WangWenru SuYuan-Ping HanHuimin FanZhongmin LiuWilliam StohlSong Guo Zheng<h4>Background</h4>BAFF, in addition to promoting B cell survival and differentiation, may affect T cells. The objective of this study was to determine the effect of BAFF on Th17 cell generation and its ramifications for the Th17 cell-driven disease, EAE.<h4>Methodology/principal findings</h4>Th17 cells were increased in BAFF-Tg B6 (B6.BTg) mice and decreased in B6.Baff(-/-) mice. Th17 cells in B6.Baff(-/-) mice bearing a BAFF Tg (B6.Baff(-/-).BTg mice) were identical to those in B6.BTg mice, indicating that membrane BAFF is dispensable for Th17 cell generation as long as soluble BAFF is plentiful. In T + non-T cell criss-cross co-cultures, Th17 cell generation was greatest in cultures containing B6.BTg T cells and lowest in cultures containing B6.Baff(-/-) T cells, regardless of the source of non-T cells. In cultures containing only T cells, Th17 cell generation followed an identical pattern. CD4(+) cell expression of CD126 (IL-6R α chain) was increased in B6.BTg mice and decreased in B6.Baff(-/-) mice, and activation of STAT3 following stimulation with IL-6 + TGF-β was also greatest in B6.BTg cells and lowest in B6.Baff(-/-) cells. EAE was clinically and pathologically most severe in B6.BTg mice and least severe in B6.Baff(-/-) mice and correlated with MOG(35-55) peptide-induced Th17 cell responses.<h4>Conclusions/significance</h4>Collectively, these findings document a contribution of BAFF to pathogenic Th17 cell responses and suggest that BAFF antagonism may be efficacious in Th17 cell-driven diseases.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023629&type=printable |
| spellingShingle | Xiaohui Zhou Zanxian Xia Qin Lan Julie Wang Wenru Su Yuan-Ping Han Huimin Fan Zhongmin Liu William Stohl Song Guo Zheng BAFF promotes Th17 cells and aggravates experimental autoimmune encephalomyelitis. PLoS ONE |
| title | BAFF promotes Th17 cells and aggravates experimental autoimmune encephalomyelitis. |
| title_full | BAFF promotes Th17 cells and aggravates experimental autoimmune encephalomyelitis. |
| title_fullStr | BAFF promotes Th17 cells and aggravates experimental autoimmune encephalomyelitis. |
| title_full_unstemmed | BAFF promotes Th17 cells and aggravates experimental autoimmune encephalomyelitis. |
| title_short | BAFF promotes Th17 cells and aggravates experimental autoimmune encephalomyelitis. |
| title_sort | baff promotes th17 cells and aggravates experimental autoimmune encephalomyelitis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023629&type=printable |
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