In vitro cytotoxicity assessment of biosynthesized Apis mellifera bee venom nanoparticles (BVNPs) against MCF-7 breast cancer cell lines
Abstract In this work, we reported the synthesis of honey bee (Apis mellifera) venom-derived nanoparticles via a hydrothermal method. This method not only ensures the preservation of the bee venom’s bioactive components but also enhances their potential stability, thus broadening the scope for their...
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Springer
2024-10-01
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| Series: | Discover Nano |
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| Online Access: | https://doi.org/10.1186/s11671-024-04123-4 |
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| author | Vikram Jadhav Arun Bhagare Ashwini Palake Kisan Kodam Akshay Dhaygude Anant Kardel Dnyaneshwar Lokhande Jayraj Aher |
| author_facet | Vikram Jadhav Arun Bhagare Ashwini Palake Kisan Kodam Akshay Dhaygude Anant Kardel Dnyaneshwar Lokhande Jayraj Aher |
| author_sort | Vikram Jadhav |
| collection | DOAJ |
| description | Abstract In this work, we reported the synthesis of honey bee (Apis mellifera) venom-derived nanoparticles via a hydrothermal method. This method not only ensures the preservation of the bee venom’s bioactive components but also enhances their potential stability, thus broadening the scope for their applications in the biomedicinal field. The synthesis method started with the homogenization suspension of bee venom, followed by its hydrothermal process to synthesize bee venom nanoparticles (BVNPs). The successful synthesis of BVNPs was characterized using various characteristic techniques such as Ultraviolet–visible (UV–Vis) spectroscopy, Fourier Transforms Infrared (FTIR) Spectroscopy, Zeta Potential (ZP), Liquid Chromatography-Mass Spectrometry (LCMS), and Transmission Electron Microscopy (TEM). The synthesis of BVNPs through biosynthesis is shown by the visible violet-brown color development at 347 nm by UV–Vis spectroscopy. FTIR analysis revealed the presence of several functional groups in the BVNPs, including alcohols (–OH), phenols (C6H5–), carboxylic acids (–COOH), amines (–NH2, –NH–), aldehydes (–CHO), ketones (–CO–), nitriles (–CN), amides (–CO–N–), imines (–CNH–), esters (–COO–), and polysaccharides. These functional groups, as confirmed by their specific stretching and bending vibrational modes, contribute to the diverse biological activities of BVNPs, including cytotoxicity against MCF-7 breast cancer cells. The ZP of the BVNPs indicated good colloidal stability at − 45 mV. LCMS analysis confirmed the presence of major bioactive molecules, including melittin & apamin and TEM analysis shows the BVNPs exhibited a quasi-spherical shape with good dispersion, the average size was approximately 25 nm, with some being smaller (quantum dots) and interplanar spacing of 0.236 nm indicated a highly ordered crystalline structure. Moreover, the anticancer efficacy of the BVNPs was ascertained through in vitro assays against MCF-7 breast cancer cells, showing a dose-dependent cytotoxic effect. The findings of this study underscore the viability of hydrothermal synthesis in producing biologically active and structurally stable BVNPs, with a significant potential for anticancer activities. Graphical Abstract |
| format | Article |
| id | doaj-art-1c14b70c5cd54cdd8a3b855bca483cb0 |
| institution | OA Journals |
| issn | 2731-9229 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Springer |
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| spelling | doaj-art-1c14b70c5cd54cdd8a3b855bca483cb02025-08-20T02:17:48ZengSpringerDiscover Nano2731-92292024-10-0119111510.1186/s11671-024-04123-4In vitro cytotoxicity assessment of biosynthesized Apis mellifera bee venom nanoparticles (BVNPs) against MCF-7 breast cancer cell linesVikram Jadhav0Arun Bhagare1Ashwini Palake2Kisan Kodam3Akshay Dhaygude4Anant Kardel5Dnyaneshwar Lokhande6Jayraj Aher7Department of Chemistry, M. V. P. Samaj’s K. K. Wagh Arts, Science, and Commerce CollegeDepartment of Chemistry, M. V. P. Samaj’s K. K. Wagh Arts, Science, and Commerce CollegeBiochemistry Division, Department of Chemistry, Savitribai Phule Pune UniversityBiochemistry Division, Department of Chemistry, Savitribai Phule Pune UniversityDepartment of Chemistry, M. V. P. Samaj’s K. K. Wagh Arts, Science, and Commerce CollegeDepartment of Chemistry, M. V. P. Samaj’s K. K. Wagh Arts, Science, and Commerce CollegePost Graduate Department of Chemistry, K. R. T. Arts, B. H. Commerce, and A. M. Science CollegePost Graduate Department of Chemistry, K. R. T. Arts, B. H. Commerce, and A. M. Science CollegeAbstract In this work, we reported the synthesis of honey bee (Apis mellifera) venom-derived nanoparticles via a hydrothermal method. This method not only ensures the preservation of the bee venom’s bioactive components but also enhances their potential stability, thus broadening the scope for their applications in the biomedicinal field. The synthesis method started with the homogenization suspension of bee venom, followed by its hydrothermal process to synthesize bee venom nanoparticles (BVNPs). The successful synthesis of BVNPs was characterized using various characteristic techniques such as Ultraviolet–visible (UV–Vis) spectroscopy, Fourier Transforms Infrared (FTIR) Spectroscopy, Zeta Potential (ZP), Liquid Chromatography-Mass Spectrometry (LCMS), and Transmission Electron Microscopy (TEM). The synthesis of BVNPs through biosynthesis is shown by the visible violet-brown color development at 347 nm by UV–Vis spectroscopy. FTIR analysis revealed the presence of several functional groups in the BVNPs, including alcohols (–OH), phenols (C6H5–), carboxylic acids (–COOH), amines (–NH2, –NH–), aldehydes (–CHO), ketones (–CO–), nitriles (–CN), amides (–CO–N–), imines (–CNH–), esters (–COO–), and polysaccharides. These functional groups, as confirmed by their specific stretching and bending vibrational modes, contribute to the diverse biological activities of BVNPs, including cytotoxicity against MCF-7 breast cancer cells. The ZP of the BVNPs indicated good colloidal stability at − 45 mV. LCMS analysis confirmed the presence of major bioactive molecules, including melittin & apamin and TEM analysis shows the BVNPs exhibited a quasi-spherical shape with good dispersion, the average size was approximately 25 nm, with some being smaller (quantum dots) and interplanar spacing of 0.236 nm indicated a highly ordered crystalline structure. Moreover, the anticancer efficacy of the BVNPs was ascertained through in vitro assays against MCF-7 breast cancer cells, showing a dose-dependent cytotoxic effect. The findings of this study underscore the viability of hydrothermal synthesis in producing biologically active and structurally stable BVNPs, with a significant potential for anticancer activities. Graphical Abstracthttps://doi.org/10.1186/s11671-024-04123-4Apis melliferaBee venom nanoparticlesCytotoxicityHydrothermal synthesisAnticancer activityMCF-7 cells |
| spellingShingle | Vikram Jadhav Arun Bhagare Ashwini Palake Kisan Kodam Akshay Dhaygude Anant Kardel Dnyaneshwar Lokhande Jayraj Aher In vitro cytotoxicity assessment of biosynthesized Apis mellifera bee venom nanoparticles (BVNPs) against MCF-7 breast cancer cell lines Discover Nano Apis mellifera Bee venom nanoparticles Cytotoxicity Hydrothermal synthesis Anticancer activity MCF-7 cells |
| title | In vitro cytotoxicity assessment of biosynthesized Apis mellifera bee venom nanoparticles (BVNPs) against MCF-7 breast cancer cell lines |
| title_full | In vitro cytotoxicity assessment of biosynthesized Apis mellifera bee venom nanoparticles (BVNPs) against MCF-7 breast cancer cell lines |
| title_fullStr | In vitro cytotoxicity assessment of biosynthesized Apis mellifera bee venom nanoparticles (BVNPs) against MCF-7 breast cancer cell lines |
| title_full_unstemmed | In vitro cytotoxicity assessment of biosynthesized Apis mellifera bee venom nanoparticles (BVNPs) against MCF-7 breast cancer cell lines |
| title_short | In vitro cytotoxicity assessment of biosynthesized Apis mellifera bee venom nanoparticles (BVNPs) against MCF-7 breast cancer cell lines |
| title_sort | in vitro cytotoxicity assessment of biosynthesized apis mellifera bee venom nanoparticles bvnps against mcf 7 breast cancer cell lines |
| topic | Apis mellifera Bee venom nanoparticles Cytotoxicity Hydrothermal synthesis Anticancer activity MCF-7 cells |
| url | https://doi.org/10.1186/s11671-024-04123-4 |
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