Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research
Abstract Medulloblastoma, the most prevalent brain tumor among children, requires a comprehensive understanding of its cellular characteristics for effective research and treatment. In this study, we focused on DAOY, a permanent cell line of medulloblastoma, and investigated the unique properties of...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Cancer Cell International |
| Online Access: | https://doi.org/10.1186/s12935-025-03646-9 |
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| author | Jana Klementová Šárka Jarošová Irina Danilová Markéta Farníková Josef Novotný Marie Davídková Martina Zíková |
| author_facet | Jana Klementová Šárka Jarošová Irina Danilová Markéta Farníková Josef Novotný Marie Davídková Martina Zíková |
| author_sort | Jana Klementová |
| collection | DOAJ |
| description | Abstract Medulloblastoma, the most prevalent brain tumor among children, requires a comprehensive understanding of its cellular characteristics for effective research and treatment. In this study, we focused on DAOY, a permanent cell line of medulloblastoma, and investigated the unique properties of DAOY cells when cultured as floating multicellular aggregates called spheres, as opposed to adherent monolayers. Through our comprehensive analysis, we identified distinct characteristics associated with DAOY spheres. Our findings demonstrate that DAOY spheres express markers for both neural stem cells, such as CD133 (PROM1), and differentiated neurons, exemplified by MAP2. Additionally, our investigation revealed that spheres-derived cells exhibit heightened resistance to ionizing radiation compared to adherent cells. Consequently, our results indicate that caution is advised when interpreting experimental results obtained from adherent cell cultures and extrapolating them to in vivo situations. |
| format | Article |
| id | doaj-art-1c0d81069c48411fb65d8a286088729b |
| institution | Kabale University |
| issn | 1475-2867 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj-art-1c0d81069c48411fb65d8a286088729b2025-01-26T12:53:30ZengBMCCancer Cell International1475-28672025-01-0125111510.1186/s12935-025-03646-9Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma researchJana Klementová0Šárka Jarošová1Irina Danilová2Markéta Farníková3Josef Novotný4Marie Davídková5Martina Zíková6Institute of Molecular Genetics of the Czech Academy of SciencesInstitute of Molecular Genetics of the Czech Academy of SciencesDepartment of Radiation Dosimetry, Nuclear Physics Institute of the Czech Academy of SciencesFaculty of Nuclear Sciences and Physical Engineering, Czech Technical University in PragueDepartment of Medical Physics, Na Homolce HospitalDepartment of Radiation Dosimetry, Nuclear Physics Institute of the Czech Academy of SciencesInstitute of Molecular Genetics of the Czech Academy of SciencesAbstract Medulloblastoma, the most prevalent brain tumor among children, requires a comprehensive understanding of its cellular characteristics for effective research and treatment. In this study, we focused on DAOY, a permanent cell line of medulloblastoma, and investigated the unique properties of DAOY cells when cultured as floating multicellular aggregates called spheres, as opposed to adherent monolayers. Through our comprehensive analysis, we identified distinct characteristics associated with DAOY spheres. Our findings demonstrate that DAOY spheres express markers for both neural stem cells, such as CD133 (PROM1), and differentiated neurons, exemplified by MAP2. Additionally, our investigation revealed that spheres-derived cells exhibit heightened resistance to ionizing radiation compared to adherent cells. Consequently, our results indicate that caution is advised when interpreting experimental results obtained from adherent cell cultures and extrapolating them to in vivo situations.https://doi.org/10.1186/s12935-025-03646-9 |
| spellingShingle | Jana Klementová Šárka Jarošová Irina Danilová Markéta Farníková Josef Novotný Marie Davídková Martina Zíková Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research Cancer Cell International |
| title | Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research |
| title_full | Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research |
| title_fullStr | Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research |
| title_full_unstemmed | Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research |
| title_short | Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research |
| title_sort | comparative analysis of pediatric shh medulloblastoma daoy spheres and adherent monolayers implications for medulloblastoma research |
| url | https://doi.org/10.1186/s12935-025-03646-9 |
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