Immunosuppressive Activity of Size-Controlled PEG-PLGA Nanoparticles Containing Encapsulated Cyclosporine A
We encapsulated cyclosporine A (CsA) in poly(ethylene glycol)-b-poly(d,l-lactide-co-glycolide) (PEG-PLGA) nanoparticles (NPs) by nanoprecipitation of CsA and PEG-PLGA. The resulting CsA/PEG-PLGA-NPs were <100 nm in diameter with a narrow particle size distribution. The NP size could be controlled...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Transplantation |
| Online Access: | http://dx.doi.org/10.1155/2012/896141 |
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| author | Li Tang Jamil Azzi Mincheol Kwon Marwan Mounayar Rong Tong Qian Yin Robert Moore Nikolaos Skartsis Timothy M. Fan Reza Abdi Jianjun Cheng |
| author_facet | Li Tang Jamil Azzi Mincheol Kwon Marwan Mounayar Rong Tong Qian Yin Robert Moore Nikolaos Skartsis Timothy M. Fan Reza Abdi Jianjun Cheng |
| author_sort | Li Tang |
| collection | DOAJ |
| description | We encapsulated cyclosporine A (CsA) in poly(ethylene glycol)-b-poly(d,l-lactide-co-glycolide) (PEG-PLGA) nanoparticles (NPs) by nanoprecipitation of CsA and PEG-PLGA. The resulting CsA/PEG-PLGA-NPs were <100 nm in diameter with a narrow particle size distribution. The NP size could be controlled by tuning the polymer concentration, solvent, or water/solvent ratio during formulation. The PEGylated NPs maintained non-aggregated in salt solution. Solid NPs lyoprotected with bovine serum albumin were prepared for the convenience of storage and transportation. The release kinetics of CsA (55.6% released on Day 1) showed potential for maintaining therapeutic CsA concentrations in vivo. In T-cell assays, both free CsA and CsA/PEG-PLGA-NPs suppressed T-cell proliferation and production of inflammatory cytokines dose dependently. In a mixed lymphocyte reaction assay, the IC50 values for free CsA and CsA/PEG-PLGA-NPs were found to be 30 and 35 ng/mL, respectively. This nanoparticulate CsA delivery technology constitutes a strong basis for future targeted delivery of immunosuppressive drugs with improved efficiency and potentially reduced toxicity. |
| format | Article |
| id | doaj-art-1c09ecdf1ef148ac9ee459d0cb99d0e1 |
| institution | OA Journals |
| issn | 2090-0007 2090-0015 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Transplantation |
| spelling | doaj-art-1c09ecdf1ef148ac9ee459d0cb99d0e12025-08-20T02:01:51ZengWileyJournal of Transplantation2090-00072090-00152012-01-01201210.1155/2012/896141896141Immunosuppressive Activity of Size-Controlled PEG-PLGA Nanoparticles Containing Encapsulated Cyclosporine ALi Tang0Jamil Azzi1Mincheol Kwon2Marwan Mounayar3Rong Tong4Qian Yin5Robert Moore6Nikolaos Skartsis7Timothy M. Fan8Reza Abdi9Jianjun Cheng10Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USATransplantation Research Center, Renal Division, Brigham and Women's Hospital; Children's Hospital Boston, Harvard Medical School, Boston, MA 02139, USADepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USATransplantation Research Center, Renal Division, Brigham and Women's Hospital; Children's Hospital Boston, Harvard Medical School, Boston, MA 02139, USADepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USADepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USATransplantation Research Center, Renal Division, Brigham and Women's Hospital; Children's Hospital Boston, Harvard Medical School, Boston, MA 02139, USATransplantation Research Center, Renal Division, Brigham and Women's Hospital; Children's Hospital Boston, Harvard Medical School, Boston, MA 02139, USADepartment of Veterinary Clinical Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USATransplantation Research Center, Renal Division, Brigham and Women's Hospital; Children's Hospital Boston, Harvard Medical School, Boston, MA 02139, USADepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USAWe encapsulated cyclosporine A (CsA) in poly(ethylene glycol)-b-poly(d,l-lactide-co-glycolide) (PEG-PLGA) nanoparticles (NPs) by nanoprecipitation of CsA and PEG-PLGA. The resulting CsA/PEG-PLGA-NPs were <100 nm in diameter with a narrow particle size distribution. The NP size could be controlled by tuning the polymer concentration, solvent, or water/solvent ratio during formulation. The PEGylated NPs maintained non-aggregated in salt solution. Solid NPs lyoprotected with bovine serum albumin were prepared for the convenience of storage and transportation. The release kinetics of CsA (55.6% released on Day 1) showed potential for maintaining therapeutic CsA concentrations in vivo. In T-cell assays, both free CsA and CsA/PEG-PLGA-NPs suppressed T-cell proliferation and production of inflammatory cytokines dose dependently. In a mixed lymphocyte reaction assay, the IC50 values for free CsA and CsA/PEG-PLGA-NPs were found to be 30 and 35 ng/mL, respectively. This nanoparticulate CsA delivery technology constitutes a strong basis for future targeted delivery of immunosuppressive drugs with improved efficiency and potentially reduced toxicity.http://dx.doi.org/10.1155/2012/896141 |
| spellingShingle | Li Tang Jamil Azzi Mincheol Kwon Marwan Mounayar Rong Tong Qian Yin Robert Moore Nikolaos Skartsis Timothy M. Fan Reza Abdi Jianjun Cheng Immunosuppressive Activity of Size-Controlled PEG-PLGA Nanoparticles Containing Encapsulated Cyclosporine A Journal of Transplantation |
| title | Immunosuppressive Activity of Size-Controlled PEG-PLGA Nanoparticles Containing Encapsulated Cyclosporine A |
| title_full | Immunosuppressive Activity of Size-Controlled PEG-PLGA Nanoparticles Containing Encapsulated Cyclosporine A |
| title_fullStr | Immunosuppressive Activity of Size-Controlled PEG-PLGA Nanoparticles Containing Encapsulated Cyclosporine A |
| title_full_unstemmed | Immunosuppressive Activity of Size-Controlled PEG-PLGA Nanoparticles Containing Encapsulated Cyclosporine A |
| title_short | Immunosuppressive Activity of Size-Controlled PEG-PLGA Nanoparticles Containing Encapsulated Cyclosporine A |
| title_sort | immunosuppressive activity of size controlled peg plga nanoparticles containing encapsulated cyclosporine a |
| url | http://dx.doi.org/10.1155/2012/896141 |
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