Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat Conditions

Understanding how habitat conditions drive morphological adaptations in animals is critical in ecology, yet amphibian studies remain limited. This study investigated intraspecific variation in ecomorphological traits of three montane stream-dwelling frogs (<i>Quasipaa boulengeri</i>, <...

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Main Authors: Xiwen Peng, Da Kang, Guangfeng Chen, Suwen Hu, Zijian Sun, Tian Zhao
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/15/2243
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author Xiwen Peng
Da Kang
Guangfeng Chen
Suwen Hu
Zijian Sun
Tian Zhao
author_facet Xiwen Peng
Da Kang
Guangfeng Chen
Suwen Hu
Zijian Sun
Tian Zhao
author_sort Xiwen Peng
collection DOAJ
description Understanding how habitat conditions drive morphological adaptations in animals is critical in ecology, yet amphibian studies remain limited. This study investigated intraspecific variation in ecomorphological traits of three montane stream-dwelling frogs (<i>Quasipaa boulengeri</i>, <i>Amolops sinensis</i>, and <i>Odorrana margaratae</i>) across elevation gradients in Tianping Mountain, China. Using morphological measurements and environmental variables collected from ten transects, we analyzed functional traits related to feeding and locomotion and assessed their associations with microhabitat variables. Significant trait differences between low- and high-elevation groups were detected only in <i>Q. boulengeri</i>, with high-elevation individuals exhibiting greater body mass and shorter hindlimbs. Redundancy analysis demonstrated that microhabitat variables, particularly air humidity, flow rate, and rock coverage, were linked to trait variations. For example, air humidity and flow rate significantly influenced <i>Q. boulengeri</i>’s body and limb proportions, while flow rate affected <i>A. sinensis</i>’s snout and limb morphology. In addition, sex and seasonal effects were also associated with trait variations. These results underscore amphibians’ phenotypic plasticity in response to the environment and highlight the role of microhabitat complexity in shaping traits. By linking habitat heterogeneity to eco-morphology, this study advocates for conservation strategies that preserve varied stream environments to support amphibian resilience amid environmental changes.
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publishDate 2025-07-01
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spelling doaj-art-1c057dcb3bea48a1b7cd247e8f8a8fe82025-08-20T03:36:32ZengMDPI AGAnimals2076-26152025-07-011515224310.3390/ani15152243Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat ConditionsXiwen Peng0Da Kang1Guangfeng Chen2Suwen Hu3Zijian Sun4Tian Zhao5College of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaUnderstanding how habitat conditions drive morphological adaptations in animals is critical in ecology, yet amphibian studies remain limited. This study investigated intraspecific variation in ecomorphological traits of three montane stream-dwelling frogs (<i>Quasipaa boulengeri</i>, <i>Amolops sinensis</i>, and <i>Odorrana margaratae</i>) across elevation gradients in Tianping Mountain, China. Using morphological measurements and environmental variables collected from ten transects, we analyzed functional traits related to feeding and locomotion and assessed their associations with microhabitat variables. Significant trait differences between low- and high-elevation groups were detected only in <i>Q. boulengeri</i>, with high-elevation individuals exhibiting greater body mass and shorter hindlimbs. Redundancy analysis demonstrated that microhabitat variables, particularly air humidity, flow rate, and rock coverage, were linked to trait variations. For example, air humidity and flow rate significantly influenced <i>Q. boulengeri</i>’s body and limb proportions, while flow rate affected <i>A. sinensis</i>’s snout and limb morphology. In addition, sex and seasonal effects were also associated with trait variations. These results underscore amphibians’ phenotypic plasticity in response to the environment and highlight the role of microhabitat complexity in shaping traits. By linking habitat heterogeneity to eco-morphology, this study advocates for conservation strategies that preserve varied stream environments to support amphibian resilience amid environmental changes.https://www.mdpi.com/2076-2615/15/15/2243functional morphologyphenotypic plasticityamphibian conservationmicrohabitat variables
spellingShingle Xiwen Peng
Da Kang
Guangfeng Chen
Suwen Hu
Zijian Sun
Tian Zhao
Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat Conditions
Animals
functional morphology
phenotypic plasticity
amphibian conservation
microhabitat variables
title Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat Conditions
title_full Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat Conditions
title_fullStr Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat Conditions
title_full_unstemmed Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat Conditions
title_short Intraspecific Variations in Ecomorphological Functional Traits of Montane Stream-Dwelling Frogs Were Driven by Their Microhabitat Conditions
title_sort intraspecific variations in ecomorphological functional traits of montane stream dwelling frogs were driven by their microhabitat conditions
topic functional morphology
phenotypic plasticity
amphibian conservation
microhabitat variables
url https://www.mdpi.com/2076-2615/15/15/2243
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