Antiproliferative Activity of Crude Marcescin on Human Laryngx Epidermoid Carcinoma (Hep-2) Cell Line in Vitro Study
Marcescin is a bacteriocin that is extracted from isolates of Serratia marcescens, which is a gram-negative bacterium that belongs to the family Enterobacteraeaceae and is considered a nosocomial pathogenic bacteria. In this research, the toxic effect of crude marcescin extracted from the S. marces...
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University of Baghdad
2025-07-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
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| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3742 |
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| author | Riad M. Abdulredha Hind Hussein Obaid |
| author_facet | Riad M. Abdulredha Hind Hussein Obaid |
| author_sort | Riad M. Abdulredha |
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Marcescin is a bacteriocin that is extracted from isolates of Serratia marcescens, which is a gram-negative bacterium that belongs to the family Enterobacteraeaceae and is considered a nosocomial pathogenic bacteria. In this research, the toxic effect of crude marcescin extracted from the S. marcescens bacteria on the human laryngx epidermoid carcinoma (Hep-2) cell line was investigated. S. marcescens was isolated from blood, urine, and stool samples of patients from Nursing House Hospital, Baghdad Teaching Hospital, and Teaching Laboratories of Medical City by using a series of dilutions to concentrations of (0.00, 1.95, 3.9, 7.8, 15.62, 31.25, 62.5, 125, 250, 500, and 1000) µg/ml and an exposure time of (24, 48, and 72) hours. The results showed significant differences (P≤0.05) in the IR values (100–2%) at the concentrations (1000–1.95) µg/ml when compared with the control (100%); there were also significant differences after 48 and 72 hours. The results showed that high concentrations caused a high rate of inhibition at concentrations (1000, 500, and 250) µg/m where the rate of inhibition was (100, 67, and 58) after 24 hours, then the rate of inhibition began to decrease gradually, reaching 2% at the lowest concentration of 1.95. It is clear that Hep-2 cells are sensitive to marcescin, and their sensitivity increases exponentially with increasing concentration and time of exposure
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| format | Article |
| id | doaj-art-1c01885bf36b4bfe994712752a8d98a4 |
| institution | Kabale University |
| issn | 1609-4042 2521-3407 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | University of Baghdad |
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| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj-art-1c01885bf36b4bfe994712752a8d98a42025-08-20T03:51:19ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072025-07-0138310.30526/38.3.3742Antiproliferative Activity of Crude Marcescin on Human Laryngx Epidermoid Carcinoma (Hep-2) Cell Line in Vitro StudyRiad M. Abdulredha0https://orcid.org/0009-0007-7966-8458Hind Hussein Obaid1https://orcid.org/0000-0002-2046-7834Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Marcescin is a bacteriocin that is extracted from isolates of Serratia marcescens, which is a gram-negative bacterium that belongs to the family Enterobacteraeaceae and is considered a nosocomial pathogenic bacteria. In this research, the toxic effect of crude marcescin extracted from the S. marcescens bacteria on the human laryngx epidermoid carcinoma (Hep-2) cell line was investigated. S. marcescens was isolated from blood, urine, and stool samples of patients from Nursing House Hospital, Baghdad Teaching Hospital, and Teaching Laboratories of Medical City by using a series of dilutions to concentrations of (0.00, 1.95, 3.9, 7.8, 15.62, 31.25, 62.5, 125, 250, 500, and 1000) µg/ml and an exposure time of (24, 48, and 72) hours. The results showed significant differences (P≤0.05) in the IR values (100–2%) at the concentrations (1000–1.95) µg/ml when compared with the control (100%); there were also significant differences after 48 and 72 hours. The results showed that high concentrations caused a high rate of inhibition at concentrations (1000, 500, and 250) µg/m where the rate of inhibition was (100, 67, and 58) after 24 hours, then the rate of inhibition began to decrease gradually, reaching 2% at the lowest concentration of 1.95. It is clear that Hep-2 cells are sensitive to marcescin, and their sensitivity increases exponentially with increasing concentration and time of exposure https://jih.uobaghdad.edu.iq/index.php/j/article/view/3742Serratia marcescensMarcescinCytotoxicityHep-2 cell lineInhibition rate |
| spellingShingle | Riad M. Abdulredha Hind Hussein Obaid Antiproliferative Activity of Crude Marcescin on Human Laryngx Epidermoid Carcinoma (Hep-2) Cell Line in Vitro Study Ibn Al-Haitham Journal for Pure and Applied Sciences Serratia marcescens Marcescin Cytotoxicity Hep-2 cell line Inhibition rate |
| title | Antiproliferative Activity of Crude Marcescin on Human Laryngx Epidermoid Carcinoma (Hep-2) Cell Line in Vitro Study |
| title_full | Antiproliferative Activity of Crude Marcescin on Human Laryngx Epidermoid Carcinoma (Hep-2) Cell Line in Vitro Study |
| title_fullStr | Antiproliferative Activity of Crude Marcescin on Human Laryngx Epidermoid Carcinoma (Hep-2) Cell Line in Vitro Study |
| title_full_unstemmed | Antiproliferative Activity of Crude Marcescin on Human Laryngx Epidermoid Carcinoma (Hep-2) Cell Line in Vitro Study |
| title_short | Antiproliferative Activity of Crude Marcescin on Human Laryngx Epidermoid Carcinoma (Hep-2) Cell Line in Vitro Study |
| title_sort | antiproliferative activity of crude marcescin on human laryngx epidermoid carcinoma hep 2 cell line in vitro study |
| topic | Serratia marcescens Marcescin Cytotoxicity Hep-2 cell line Inhibition rate |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3742 |
| work_keys_str_mv | AT riadmabdulredha antiproliferativeactivityofcrudemarcescinonhumanlaryngxepidermoidcarcinomahep2celllineinvitrostudy AT hindhusseinobaid antiproliferativeactivityofcrudemarcescinonhumanlaryngxepidermoidcarcinomahep2celllineinvitrostudy |