circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC
ABSTRACT Background Lung cancer has a notably high incidence and mortality rate, and understanding its occurrence and development is crucial for effective treatment. Circular RNA is closely associated with tumor progression, playing a role in tumorigenesis and development by regulating gene expressi...
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Wiley
2025-03-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.70729 |
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| author | Wei Chen Weijun Zhao Xianqiao Wu Tianzheng Fang Ziyuan Chen Zixuan Chen Wenmin Su Xiaodong Zhao Yuanyuan Hu Yiping Xu Shuai Fang Chengwei Zhou |
| author_facet | Wei Chen Weijun Zhao Xianqiao Wu Tianzheng Fang Ziyuan Chen Zixuan Chen Wenmin Su Xiaodong Zhao Yuanyuan Hu Yiping Xu Shuai Fang Chengwei Zhou |
| author_sort | Wei Chen |
| collection | DOAJ |
| description | ABSTRACT Background Lung cancer has a notably high incidence and mortality rate, and understanding its occurrence and development is crucial for effective treatment. Circular RNA is closely associated with tumor progression, playing a role in tumorigenesis and development by regulating gene expression and influencing cell proliferation and apoptosis. This study aims to explore the circFOXK2 role in NSCLC occurrence and development and to elucidate its underlying mechanisms. Methods qRT‐PCR and Western Blot analyzed the expressions of circFOXK2, STMN1, and PABPCA in NSCLC cell lines, as well as their relationships. The roles of circFOXK2 and STMN1 in the proliferation, invasion, and migration of NSCLC cells were assessed through CCK8, EDU, and Transwell experiments. RNA pulldown assays with mass spectrometry elucidated the RNA‐binding proteins of circFOXK2. Subcutaneous tumorigenesis and tail vein lung metastasis experiments analyzed the impact of circFOXK2 on tumor growth and metastasis in vivo. Results In this study, we identified circFOXK2, which is significantly overexpressed in NSCLC, through bioinformatics screening. Elevated levels of circFOXK2 enhance the growth and metastasis of NSCLC cells. Furthermore, through experiments such as co‐IP and mass spectrometry, we found that circFOXK2 interacts with PABPC1 to form a complex, which correlates positively with the progression and metastasis of tumors. Simultaneously, the circFOXK2/PABPC1 complex increases the stability of STMN1 mRNA, thereby promoting the transcription and translation of STMN1. Our experimental results indicate that the oncogenic effect of circFOXK2 requires the assistance of STMN1. Conclusions In summary, we have demonstrated the significant role of circFOXK2/PABPC1 in regulating STMN1 expression in NSCLC. |
| format | Article |
| id | doaj-art-1bfe0b2cef884cde8914fe07b5eeeb5e |
| institution | OA Journals |
| issn | 2045-7634 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-1bfe0b2cef884cde8914fe07b5eeeb5e2025-08-20T02:05:21ZengWileyCancer Medicine2045-76342025-03-01145n/an/a10.1002/cam4.70729circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLCWei Chen0Weijun Zhao1Xianqiao Wu2Tianzheng Fang3Ziyuan Chen4Zixuan Chen5Wenmin Su6Xiaodong Zhao7Yuanyuan Hu8Yiping Xu9Shuai Fang10Chengwei Zhou11Thoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaThoracic Surgery Department The First Affiliated Hospital of Ningbo University Ningbo Zhejiang People's Republic of ChinaABSTRACT Background Lung cancer has a notably high incidence and mortality rate, and understanding its occurrence and development is crucial for effective treatment. Circular RNA is closely associated with tumor progression, playing a role in tumorigenesis and development by regulating gene expression and influencing cell proliferation and apoptosis. This study aims to explore the circFOXK2 role in NSCLC occurrence and development and to elucidate its underlying mechanisms. Methods qRT‐PCR and Western Blot analyzed the expressions of circFOXK2, STMN1, and PABPCA in NSCLC cell lines, as well as their relationships. The roles of circFOXK2 and STMN1 in the proliferation, invasion, and migration of NSCLC cells were assessed through CCK8, EDU, and Transwell experiments. RNA pulldown assays with mass spectrometry elucidated the RNA‐binding proteins of circFOXK2. Subcutaneous tumorigenesis and tail vein lung metastasis experiments analyzed the impact of circFOXK2 on tumor growth and metastasis in vivo. Results In this study, we identified circFOXK2, which is significantly overexpressed in NSCLC, through bioinformatics screening. Elevated levels of circFOXK2 enhance the growth and metastasis of NSCLC cells. Furthermore, through experiments such as co‐IP and mass spectrometry, we found that circFOXK2 interacts with PABPC1 to form a complex, which correlates positively with the progression and metastasis of tumors. Simultaneously, the circFOXK2/PABPC1 complex increases the stability of STMN1 mRNA, thereby promoting the transcription and translation of STMN1. Our experimental results indicate that the oncogenic effect of circFOXK2 requires the assistance of STMN1. Conclusions In summary, we have demonstrated the significant role of circFOXK2/PABPC1 in regulating STMN1 expression in NSCLC.https://doi.org/10.1002/cam4.70729circFOXK2NSCLCPABPC1RNA–protein interactionSTMN1 |
| spellingShingle | Wei Chen Weijun Zhao Xianqiao Wu Tianzheng Fang Ziyuan Chen Zixuan Chen Wenmin Su Xiaodong Zhao Yuanyuan Hu Yiping Xu Shuai Fang Chengwei Zhou circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC Cancer Medicine circFOXK2 NSCLC PABPC1 RNA–protein interaction STMN1 |
| title | circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC |
| title_full | circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC |
| title_fullStr | circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC |
| title_full_unstemmed | circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC |
| title_short | circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC |
| title_sort | circfoxk2 stabilizes stmn1 mrna via pabpc1 to promote the progression of nsclc |
| topic | circFOXK2 NSCLC PABPC1 RNA–protein interaction STMN1 |
| url | https://doi.org/10.1002/cam4.70729 |
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