Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model
Abstract Background The target and mechanism of oral traditional Chinese medicine (TCM) have been important research directions for a long time. The close relationship between osteoporosis and gut microbiota (GM) has been confirmed. However, the relevance of oral TCM and the “Gut-Bone Axis” is still...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-025-05504-w |
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| author | Junjie Li HaomingYou Yucheng Hu Ruxu Li Tianxin Ouyang Qiang Ran Guilong Zhang Yong Huang |
| author_facet | Junjie Li HaomingYou Yucheng Hu Ruxu Li Tianxin Ouyang Qiang Ran Guilong Zhang Yong Huang |
| author_sort | Junjie Li |
| collection | DOAJ |
| description | Abstract Background The target and mechanism of oral traditional Chinese medicine (TCM) have been important research directions for a long time. The close relationship between osteoporosis and gut microbiota (GM) has been confirmed. However, the relevance of oral TCM and the “Gut-Bone Axis” is still poorly understood. Methods Twenty-one SPF C57BL/6J female mice were divided into sham (Sham), ovariectomized (OVX), and Zuo-Gui-Wan-treated (ZGW, 1.4 g/kg) groups. The osteoporosis mouse model was established through ovariectomy. After eight weeks of Zuo-Gui-Wan treatment via gavage, serum calcium, phosphorus, ALT, AST, CREA, and other biochemical indicators were measured. Subsequently, Micro-CT, HE staining, and analysis of gut microbiota were conducted to further explore the potential mechanism. Results The anti-osteoporotic effects of ZGW were confirmed through micro-CT, histological, and biochemical tests in an OVX-induced osteoporosis mouse model. ZGW treatment also alters the diversity and composition of the gut microbiota and altered the Firmicutes/Bacteroidetes ratio. Further analysis reveals a correlation between specific bacterial groups and serum indicators. Mfuzz clustering analysis and metagenomeSeq analysis identified important microbiota species that were rescued or modulated by ZGW treatment. Conclusion These findings suggest that changes in gut microbiota abundance may be linked to ZGW’s ability to improve osteoporosis. This study provides new insights into how ZGW treats osteoporosis, though further research is needed to clarify the mechanisms by which specific gut microbiota influence bone health. |
| format | Article |
| id | doaj-art-1be913174c6045849e4b449fe770ef48 |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-1be913174c6045849e4b449fe770ef482025-02-02T12:34:06ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2025-02-0120111210.1186/s13018-025-05504-wEffects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse modelJunjie Li0HaomingYou1Yucheng Hu2Ruxu Li3Tianxin Ouyang4Qiang Ran5Guilong Zhang6Yong Huang7Hospital of Chengdu University of Traditional Chinese MedicineHospital of Chengdu University of Traditional Chinese MedicineHospital of Chengdu University of Traditional Chinese MedicineHospital of Chengdu University of Traditional Chinese MedicineHospital of Chengdu University of Traditional Chinese MedicineHospital of Chengdu University of Traditional Chinese MedicineDepartment of Orthopedics, Hospital of Chengdu University of Traditional Chinese MedicineDepartment of Orthopedics, Hospital of Chengdu University of Traditional Chinese MedicineAbstract Background The target and mechanism of oral traditional Chinese medicine (TCM) have been important research directions for a long time. The close relationship between osteoporosis and gut microbiota (GM) has been confirmed. However, the relevance of oral TCM and the “Gut-Bone Axis” is still poorly understood. Methods Twenty-one SPF C57BL/6J female mice were divided into sham (Sham), ovariectomized (OVX), and Zuo-Gui-Wan-treated (ZGW, 1.4 g/kg) groups. The osteoporosis mouse model was established through ovariectomy. After eight weeks of Zuo-Gui-Wan treatment via gavage, serum calcium, phosphorus, ALT, AST, CREA, and other biochemical indicators were measured. Subsequently, Micro-CT, HE staining, and analysis of gut microbiota were conducted to further explore the potential mechanism. Results The anti-osteoporotic effects of ZGW were confirmed through micro-CT, histological, and biochemical tests in an OVX-induced osteoporosis mouse model. ZGW treatment also alters the diversity and composition of the gut microbiota and altered the Firmicutes/Bacteroidetes ratio. Further analysis reveals a correlation between specific bacterial groups and serum indicators. Mfuzz clustering analysis and metagenomeSeq analysis identified important microbiota species that were rescued or modulated by ZGW treatment. Conclusion These findings suggest that changes in gut microbiota abundance may be linked to ZGW’s ability to improve osteoporosis. This study provides new insights into how ZGW treats osteoporosis, though further research is needed to clarify the mechanisms by which specific gut microbiota influence bone health.https://doi.org/10.1186/s13018-025-05504-wChinese medicineZGWOsteoporosisBone microstructureGut microbiota |
| spellingShingle | Junjie Li HaomingYou Yucheng Hu Ruxu Li Tianxin Ouyang Qiang Ran Guilong Zhang Yong Huang Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model Journal of Orthopaedic Surgery and Research Chinese medicine ZGW Osteoporosis Bone microstructure Gut microbiota |
| title | Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model |
| title_full | Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model |
| title_fullStr | Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model |
| title_full_unstemmed | Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model |
| title_short | Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model |
| title_sort | effects of traditional chinese medicine zuo gui wan on gut microbiota in an osteoporotic mouse model |
| topic | Chinese medicine ZGW Osteoporosis Bone microstructure Gut microbiota |
| url | https://doi.org/10.1186/s13018-025-05504-w |
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