Albiflorin inhibits inflammation to improve liver fibrosis by targeting the CXCL12/CXCR4 axis in mice

Liver fibrosis is a common pathological feature of chronic hepatic injury that currently lacks effective therapeutic interventions. Albiflorin (ALB), a pinane-type monoterpene derived from Paeonia lactiflora Pall, has notable anti-inflammatory and hepatoprotective effects. However, the potential rol...

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Main Authors: Lingjie Meng, Huijing Lv, Anli Liu, Qian Cao, Xinyi Du, Chengjin Li, Qinggui Li, Qingqing Luo, Yi Xiao
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Pharmacology
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Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2025.1577201/full
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author Lingjie Meng
Lingjie Meng
Huijing Lv
Huijing Lv
Anli Liu
Anli Liu
Qian Cao
Qian Cao
Xinyi Du
Xinyi Du
Chengjin Li
Chengjin Li
Qinggui Li
Qinggui Li
Qingqing Luo
Qingqing Luo
Yi Xiao
Yi Xiao
author_facet Lingjie Meng
Lingjie Meng
Huijing Lv
Huijing Lv
Anli Liu
Anli Liu
Qian Cao
Qian Cao
Xinyi Du
Xinyi Du
Chengjin Li
Chengjin Li
Qinggui Li
Qinggui Li
Qingqing Luo
Qingqing Luo
Yi Xiao
Yi Xiao
author_sort Lingjie Meng
collection DOAJ
description Liver fibrosis is a common pathological feature of chronic hepatic injury that currently lacks effective therapeutic interventions. Albiflorin (ALB), a pinane-type monoterpene derived from Paeonia lactiflora Pall, has notable anti-inflammatory and hepatoprotective effects. However, the potential role of ALB against liver fibrosis is largely unknown. In this study, we discovered that ALB significantly inhibited CCl4-induced liver fibrosis in mice. This was evidenced by improvements in liver and kidney function indexes, fibrosis indicators, and histopathological findings. In vitro studies also showed that ALB inhibited TGF-β1-induced LX-2 cell activation and reduced the expression of α-SMA and collagen I. Additionally, we found that ALB mitigates inflammation and ameliorates liver fibrosis by targeting the CXCL12/CXCR4 axis, as confirmed using the CXCR4 inhibitor AMD3100 in CCl4-treated mice. Notably, combining ALB with metformin (MET) enhanced the inhibition of liver fibrosis progression. These findings highlight that ALB exerts anti-liver fibrosis effects by targeting the CXCL12/CXCR4 axis, underscoring its potential as a standalone treatment or as an adjuvant therapy.
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institution OA Journals
issn 1663-9812
language English
publishDate 2025-04-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Pharmacology
spelling doaj-art-1bd8b7afcd55463fa7e9cdffa5d7a1a22025-08-20T02:20:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-04-011610.3389/fphar.2025.15772011577201Albiflorin inhibits inflammation to improve liver fibrosis by targeting the CXCL12/CXCR4 axis in miceLingjie Meng0Lingjie Meng1Huijing Lv2Huijing Lv3Anli Liu4Anli Liu5Qian Cao6Qian Cao7Xinyi Du8Xinyi Du9Chengjin Li10Chengjin Li11Qinggui Li12Qinggui Li13Qingqing Luo14Qingqing Luo15Yi Xiao16Yi Xiao17Institute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of life sciences, Zunyi Medical University, Zunyi, Guizhou, ChinaCollege of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, ChinaLiver fibrosis is a common pathological feature of chronic hepatic injury that currently lacks effective therapeutic interventions. Albiflorin (ALB), a pinane-type monoterpene derived from Paeonia lactiflora Pall, has notable anti-inflammatory and hepatoprotective effects. However, the potential role of ALB against liver fibrosis is largely unknown. In this study, we discovered that ALB significantly inhibited CCl4-induced liver fibrosis in mice. This was evidenced by improvements in liver and kidney function indexes, fibrosis indicators, and histopathological findings. In vitro studies also showed that ALB inhibited TGF-β1-induced LX-2 cell activation and reduced the expression of α-SMA and collagen I. Additionally, we found that ALB mitigates inflammation and ameliorates liver fibrosis by targeting the CXCL12/CXCR4 axis, as confirmed using the CXCR4 inhibitor AMD3100 in CCl4-treated mice. Notably, combining ALB with metformin (MET) enhanced the inhibition of liver fibrosis progression. These findings highlight that ALB exerts anti-liver fibrosis effects by targeting the CXCL12/CXCR4 axis, underscoring its potential as a standalone treatment or as an adjuvant therapy.https://www.frontiersin.org/articles/10.3389/fphar.2025.1577201/fullalbiflorinCXCL12/CXCR4 axisliver fibrosisinflammationcombination therapeutic
spellingShingle Lingjie Meng
Lingjie Meng
Huijing Lv
Huijing Lv
Anli Liu
Anli Liu
Qian Cao
Qian Cao
Xinyi Du
Xinyi Du
Chengjin Li
Chengjin Li
Qinggui Li
Qinggui Li
Qingqing Luo
Qingqing Luo
Yi Xiao
Yi Xiao
Albiflorin inhibits inflammation to improve liver fibrosis by targeting the CXCL12/CXCR4 axis in mice
Frontiers in Pharmacology
albiflorin
CXCL12/CXCR4 axis
liver fibrosis
inflammation
combination therapeutic
title Albiflorin inhibits inflammation to improve liver fibrosis by targeting the CXCL12/CXCR4 axis in mice
title_full Albiflorin inhibits inflammation to improve liver fibrosis by targeting the CXCL12/CXCR4 axis in mice
title_fullStr Albiflorin inhibits inflammation to improve liver fibrosis by targeting the CXCL12/CXCR4 axis in mice
title_full_unstemmed Albiflorin inhibits inflammation to improve liver fibrosis by targeting the CXCL12/CXCR4 axis in mice
title_short Albiflorin inhibits inflammation to improve liver fibrosis by targeting the CXCL12/CXCR4 axis in mice
title_sort albiflorin inhibits inflammation to improve liver fibrosis by targeting the cxcl12 cxcr4 axis in mice
topic albiflorin
CXCL12/CXCR4 axis
liver fibrosis
inflammation
combination therapeutic
url https://www.frontiersin.org/articles/10.3389/fphar.2025.1577201/full
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