USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION

USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION

ABSTRACT An indirect enzyme-linked immunosorbent assay (ELISA) was used to detect serum antibody in ayu, Plecoglossus altivelis, immunized against Pseudomonasplecoglossicida by immersion vaccination.  First, the procedure of the ELISA was optimized and the sensitivity was checked.  Secondly, the f...

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Main Authors: . Sukenda, Hisatsugu Wakabayashi
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2007-05-01
Series:Jurnal Akuakultur Indonesia
Online Access:https://journal.ipb.ac.id/index.php/jai/article/view/3840
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author . Sukenda
Hisatsugu Wakabayashi
author_facet . Sukenda
Hisatsugu Wakabayashi
author_sort . Sukenda
collection DOAJ
description ABSTRACT An indirect enzyme-linked immunosorbent assay (ELISA) was used to detect serum antibody in ayu, Plecoglossus altivelis, immunized against Pseudomonasplecoglossicida by immersion vaccination.  First, the procedure of the ELISA was optimized and the sensitivity was checked.  Secondly, the formalin-killed cells (FKC) of P. plecoglossicida was administered to ayu by immersion vaccination.  Two weeks after vaccination, fish were divided into two groups, one group was given booster.  The level of specific antibody production of both boostered and vaccinated only fish were statistically higher than unvaccinated control fish at the time of each blood collection.  However, the differences between the boostered and vaccinated only fish were not statistically significant. Keywords :  immunization, Pseudomonas plecoglossicida, ayu, ELISA   ABSTRAK Indirect enzyme-linked immunosorbent assay (ELISA) digunakan untuk mendeteksi antibodi pada ayu, Plecoglossus altivelis, yang diimunisasi dengan cara perendaman untuk melawan infeksi Pseudomonas plecoglossicida.  Pertama, prosedur ELISA dioptimasikan dan sensitivitas dari metode ini juga diperiksa.  Kemudian, bakteri Plecoglossus altivelis yang sudah dimatikan dengan formalin diberikan ke ikan ayu dengan vaksinasi perendaman.  Dua minggu setelah vaksinasi, ikan dibagi menjadi dua kelompok, satu kelompok diberi vaksinasi kedua.  Produksi antibodi spesifik dari ikan-ikan yang divaksinasi satu kali dengan vaksinasi dua kaii secara statistik lebih tinggi dibandingkan dengan control.  Akan tetapi, tidak ada perbedaan produksi antibodi antara ikan yarig divaksanisi satu kali dengan divaksinasi dua kali. Kata kunci :  imunisasi, Pseudomonasplecoglossicida, ayu, ELISA
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id doaj-art-1bd4cea9adba405a8a4ca8715aff29dc
institution Kabale University
issn 1412-5269
2354-6700
language English
publishDate 2007-05-01
publisher Asosiasi Sains Akuakultur Indonesia
record_format Article
series Jurnal Akuakultur Indonesia
spelling doaj-art-1bd4cea9adba405a8a4ca8715aff29dc2025-01-24T18:04:45ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002007-05-011110.19027/jai.1.39-453462USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION. Sukenda0Hisatsugu Wakabayashi1Bogor Agricultural University, Department of AquacultureDepartment of aquatic Bioscience, Graduate School ofagricultural and Life Sciences, The University of Tokyo ABSTRACT An indirect enzyme-linked immunosorbent assay (ELISA) was used to detect serum antibody in ayu, Plecoglossus altivelis, immunized against Pseudomonasplecoglossicida by immersion vaccination.  First, the procedure of the ELISA was optimized and the sensitivity was checked.  Secondly, the formalin-killed cells (FKC) of P. plecoglossicida was administered to ayu by immersion vaccination.  Two weeks after vaccination, fish were divided into two groups, one group was given booster.  The level of specific antibody production of both boostered and vaccinated only fish were statistically higher than unvaccinated control fish at the time of each blood collection.  However, the differences between the boostered and vaccinated only fish were not statistically significant. Keywords :  immunization, Pseudomonas plecoglossicida, ayu, ELISA   ABSTRAK Indirect enzyme-linked immunosorbent assay (ELISA) digunakan untuk mendeteksi antibodi pada ayu, Plecoglossus altivelis, yang diimunisasi dengan cara perendaman untuk melawan infeksi Pseudomonas plecoglossicida.  Pertama, prosedur ELISA dioptimasikan dan sensitivitas dari metode ini juga diperiksa.  Kemudian, bakteri Plecoglossus altivelis yang sudah dimatikan dengan formalin diberikan ke ikan ayu dengan vaksinasi perendaman.  Dua minggu setelah vaksinasi, ikan dibagi menjadi dua kelompok, satu kelompok diberi vaksinasi kedua.  Produksi antibodi spesifik dari ikan-ikan yang divaksinasi satu kali dengan vaksinasi dua kaii secara statistik lebih tinggi dibandingkan dengan control.  Akan tetapi, tidak ada perbedaan produksi antibodi antara ikan yarig divaksanisi satu kali dengan divaksinasi dua kali. Kata kunci :  imunisasi, Pseudomonasplecoglossicida, ayu, ELISA https://journal.ipb.ac.id/index.php/jai/article/view/3840
spellingShingle . Sukenda
Hisatsugu Wakabayashi
USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION
Jurnal Akuakultur Indonesia
title USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION
title_full USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION
title_fullStr USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION
title_full_unstemmed USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION
title_short USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION
title_sort use of an indirect enzyme linked immunosorbent assay elisa to detect antibodies in ayu plecogiossus altivelis vaccinated by immersion administration
url https://journal.ipb.ac.id/index.php/jai/article/view/3840
work_keys_str_mv AT sukenda useofanindirectenzymelinkedimmunosorbentassayelisatodetectantibodiesinayuplecogiossusaltivelisvaccinatedbyimmersionadministration
AT hisatsuguwakabayashi useofanindirectenzymelinkedimmunosorbentassayelisatodetectantibodiesinayuplecogiossusaltivelisvaccinatedbyimmersionadministration

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