Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods
Objectives: This study aimed to investigate the physical properties of Indonesian local sheep skin collagen extracted by acid and enzymatic methods. Materials and Methods: Collagen was isolated from Pure Breed Garut Sheep (Ovis aries sp.) skin, 1.5 years old. The skins were obtained from a local sl...
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Network for the Veterinarians of Bangladesh
2024-04-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | https://bdvets.org/JAVAR/?mno=175018 |
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| author | Dita Prameswari Trenggono Putri Vernanda Widya Pangestika Hanifan Ilyas Mohammad Zainal Abidin Nanung Agus Fitriyanto Yuny Erwanto |
| author_facet | Dita Prameswari Trenggono Putri Vernanda Widya Pangestika Hanifan Ilyas Mohammad Zainal Abidin Nanung Agus Fitriyanto Yuny Erwanto |
| author_sort | Dita Prameswari Trenggono Putri |
| collection | DOAJ |
| description | Objectives: This study aimed to investigate the physical properties of Indonesian local sheep skin collagen extracted by acid and enzymatic methods.
Materials and Methods: Collagen was isolated from Pure Breed Garut Sheep (Ovis aries sp.) skin, 1.5 years old. The skins were obtained from a local slaughterhouse in Cirebon, Indonesia. The solvents used were CH3COOH and three different enzymes: neutrase, alcalase, and bromelain.
Results: The highest yields of extracted collagen were bromelain-soluble collagen (BSC), which reached 37.07%. The range of Ph values for all samples started from 4.01 to 4.76. The viscosity values (cP) of acid-soluble collagen (ASC), neutrase-soluble collagen (NSC), alcalase-soluble collagen (LSC), and BSC were 3.42, 3.90, 3.45, and 3.12, respectively. Regarding SDS-PAGE analyses, Garut sheepskin collagen is categorized as collagen type I, which has a molecular weight of about 140.99 to 148.74 kDa for α1 and around 110 to 111.86 kDa for α2. The results of FTIR and DSC analyses for all samples show the same motif with commercial collagen motifs based on the literature.
Conclusion: Garut sheep skin has the potential to be an alternative raw material source for producing collagen. Collagen extracted using a combination of CH3COOH and bromelain enzyme showed the most desirable results in almost every characteristic. [J Adv Vet Anim Res 2024; 11(3.000): 722-728] |
| format | Article |
| id | doaj-art-1bd3b77616404f32a9b6126c39ac423f |
| institution | OA Journals |
| issn | 2311-7710 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj-art-1bd3b77616404f32a9b6126c39ac423f2025-08-20T02:26:37ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102024-04-0111372272810.5455/javar.2024.k823175018Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methodsDita Prameswari Trenggono Putri0Vernanda Widya Pangestika1Hanifan Ilyas2Mohammad Zainal Abidin3Nanung Agus Fitriyanto4Yuny Erwanto5Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia. & Institute for Halal Industry and System, Gadjah Mada University, Yogyakarta, Indonesia.Objectives: This study aimed to investigate the physical properties of Indonesian local sheep skin collagen extracted by acid and enzymatic methods. Materials and Methods: Collagen was isolated from Pure Breed Garut Sheep (Ovis aries sp.) skin, 1.5 years old. The skins were obtained from a local slaughterhouse in Cirebon, Indonesia. The solvents used were CH3COOH and three different enzymes: neutrase, alcalase, and bromelain. Results: The highest yields of extracted collagen were bromelain-soluble collagen (BSC), which reached 37.07%. The range of Ph values for all samples started from 4.01 to 4.76. The viscosity values (cP) of acid-soluble collagen (ASC), neutrase-soluble collagen (NSC), alcalase-soluble collagen (LSC), and BSC were 3.42, 3.90, 3.45, and 3.12, respectively. Regarding SDS-PAGE analyses, Garut sheepskin collagen is categorized as collagen type I, which has a molecular weight of about 140.99 to 148.74 kDa for α1 and around 110 to 111.86 kDa for α2. The results of FTIR and DSC analyses for all samples show the same motif with commercial collagen motifs based on the literature. Conclusion: Garut sheep skin has the potential to be an alternative raw material source for producing collagen. Collagen extracted using a combination of CH3COOH and bromelain enzyme showed the most desirable results in almost every characteristic. [J Adv Vet Anim Res 2024; 11(3.000): 722-728]https://bdvets.org/JAVAR/?mno=175018enzymatic method; acid method; collagen; characteristics; garut sheep |
| spellingShingle | Dita Prameswari Trenggono Putri Vernanda Widya Pangestika Hanifan Ilyas Mohammad Zainal Abidin Nanung Agus Fitriyanto Yuny Erwanto Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods Journal of Advanced Veterinary and Animal Research enzymatic method; acid method; collagen; characteristics; garut sheep |
| title | Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods |
| title_full | Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods |
| title_fullStr | Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods |
| title_full_unstemmed | Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods |
| title_short | Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods |
| title_sort | collagen properties of indonesian local sheepskin isolated using acid and enzymatic methods |
| topic | enzymatic method; acid method; collagen; characteristics; garut sheep |
| url | https://bdvets.org/JAVAR/?mno=175018 |
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