Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic study
Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter (CR-Ent) species remain unclear in China. In this study, we performed a genomic study on 92 isolates from Enterobacter-caused infections from a multicenter study in China. Whole genome sequencing (WGS) was us...
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2022.2148562 |
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| author | Zhichen Zhu Xiaofang Xie Hua Yu Wei Jia Bin Shan Bin Huang Fen Qu Siqiang Niu Jinnan Lv Qizhao Gao Feinan Qian Xiangxiang Tian Yaxuan Zhai Yicheng Wen Chengcheng Yang Jie Zhu Yiwei Tang Liang Chen Hong Du |
| author_facet | Zhichen Zhu Xiaofang Xie Hua Yu Wei Jia Bin Shan Bin Huang Fen Qu Siqiang Niu Jinnan Lv Qizhao Gao Feinan Qian Xiangxiang Tian Yaxuan Zhai Yicheng Wen Chengcheng Yang Jie Zhu Yiwei Tang Liang Chen Hong Du |
| author_sort | Zhichen Zhu |
| collection | DOAJ |
| description | Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter (CR-Ent) species remain unclear in China. In this study, we performed a genomic study on 92 isolates from Enterobacter-caused infections from a multicenter study in China. Whole genome sequencing (WGS) was used to determine the genome sequence of 92 non-duplicated CR-Ent strains collected from multiple tertiary health centres. The precise species of Enterobacter strains were identified by average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH). Molecular features of high-risk CR-Ent sequence type (ST) lineages and carbapenemase-encoding plasmids were determined. The result revealed that the most common human-source CR-Ent species in China was E. xiangfangensis (66/92, 71.93%), and the proportion of carbapenemase-producing Enterobacter (CP-Ent) in CR-Ent was high (72/92, 78.26%) in comparison to other global regions. Furthermore, ST171 and ST116 E. xiangfangensis were the major lineages of CP-Ent strains, and ST171 E. xiangfangensis was more likely to cause infections in older patients. Genomic analysis also highlighted the likelihood of intra-hospital/inter-hospital clonal transmission of ST171 and ST116 E. xiangfangensis. In addition, the blaNDM-harbouring IncX3-type plasmid was identified as the prevalent carbapenemase-encoding plasmid carried by CR-Ent strains, and was experimentally confirmed to be able to self-transfer with high frequency. This study detailed the genomic and clinical characteristics of CR-Ent in China in the form of multicenter for the first time. The high risk of carbapenemase-producing ST171 and ST116 E. xiangfangensis, and the blaNDM-harbouring IncX3-type plasmid were detected and emphasized. |
| format | Article |
| id | doaj-art-1bd0a9bfc1c54cc1b88cd7c62b3bfaca |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
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| series | Emerging Microbes and Infections |
| spelling | doaj-art-1bd0a9bfc1c54cc1b88cd7c62b3bfaca2025-08-20T01:49:16ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112110.1080/22221751.2022.2148562Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic studyZhichen Zhu0Xiaofang Xie1Hua Yu2Wei Jia3Bin Shan4Bin Huang5Fen Qu6Siqiang Niu7Jinnan Lv8Qizhao Gao9Feinan Qian10Xiangxiang Tian11Yaxuan Zhai12Yicheng Wen13Chengcheng Yang14Jie Zhu15Yiwei Tang16Liang Chen17Hong Du18Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, People's Republic of ChinaCenter of Medical Laboratory, General Hospital of Ningxia Medical University, Yinchuan, People’s Republic of ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of ChinaLaboratory Medicine Center, Aviation General Hospital, Beijing, People’s Republic of ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaDepartment of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USACenter for Discovery and Innovation, Hackensack-Meridian Health, Nutley, NJ, USADepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of ChinaEpidemiological characteristics and molecular features of carbapenem-resistant Enterobacter (CR-Ent) species remain unclear in China. In this study, we performed a genomic study on 92 isolates from Enterobacter-caused infections from a multicenter study in China. Whole genome sequencing (WGS) was used to determine the genome sequence of 92 non-duplicated CR-Ent strains collected from multiple tertiary health centres. The precise species of Enterobacter strains were identified by average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH). Molecular features of high-risk CR-Ent sequence type (ST) lineages and carbapenemase-encoding plasmids were determined. The result revealed that the most common human-source CR-Ent species in China was E. xiangfangensis (66/92, 71.93%), and the proportion of carbapenemase-producing Enterobacter (CP-Ent) in CR-Ent was high (72/92, 78.26%) in comparison to other global regions. Furthermore, ST171 and ST116 E. xiangfangensis were the major lineages of CP-Ent strains, and ST171 E. xiangfangensis was more likely to cause infections in older patients. Genomic analysis also highlighted the likelihood of intra-hospital/inter-hospital clonal transmission of ST171 and ST116 E. xiangfangensis. In addition, the blaNDM-harbouring IncX3-type plasmid was identified as the prevalent carbapenemase-encoding plasmid carried by CR-Ent strains, and was experimentally confirmed to be able to self-transfer with high frequency. This study detailed the genomic and clinical characteristics of CR-Ent in China in the form of multicenter for the first time. The high risk of carbapenemase-producing ST171 and ST116 E. xiangfangensis, and the blaNDM-harbouring IncX3-type plasmid were detected and emphasized.https://www.tandfonline.com/doi/10.1080/22221751.2022.2148562EnterobacterblaNDMIncX3ST171ST116carbapenemase |
| spellingShingle | Zhichen Zhu Xiaofang Xie Hua Yu Wei Jia Bin Shan Bin Huang Fen Qu Siqiang Niu Jinnan Lv Qizhao Gao Feinan Qian Xiangxiang Tian Yaxuan Zhai Yicheng Wen Chengcheng Yang Jie Zhu Yiwei Tang Liang Chen Hong Du Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic study Emerging Microbes and Infections Enterobacter blaNDM IncX3 ST171 ST116 carbapenemase |
| title | Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic study |
| title_full | Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic study |
| title_fullStr | Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic study |
| title_full_unstemmed | Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic study |
| title_short | Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic study |
| title_sort | epidemiological characteristics and molecular features of carbapenem resistant enterobacter strains in china a multicenter genomic study |
| topic | Enterobacter blaNDM IncX3 ST171 ST116 carbapenemase |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2022.2148562 |
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