Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments
Developmental studies and 3D in vitro model systems show that the production and engagement of extracellular matrix (ECM) often precede stem cell differentiation. Yet, unclear is how the ECM triggers signaling events in sequence to accommodate multistep process characteristic of differentiation. Her...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/4809673 |
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| author | Quyen A. Tran Visar Ajeti Brian T. Freeman Paul J. Campagnola Brenda M. Ogle |
| author_facet | Quyen A. Tran Visar Ajeti Brian T. Freeman Paul J. Campagnola Brenda M. Ogle |
| author_sort | Quyen A. Tran |
| collection | DOAJ |
| description | Developmental studies and 3D in vitro model systems show that the production and engagement of extracellular matrix (ECM) often precede stem cell differentiation. Yet, unclear is how the ECM triggers signaling events in sequence to accommodate multistep process characteristic of differentiation. Here, we employ transcriptome profiling and advanced imaging to delineate the specificity of ECM engagement to particular differentiation pathways and to determine whether specificity in this context is a function of long-term ECM remodeling. To this end, human mesenchymal stem cells (hMSCs) were cultured in 3D bioprinted prisms created from ECM proteins and associated controls. We found that exogenous ECM provided in 3D microenvironments at early time points impacts on the composition of microenvironments at later time points and that each evolving 3D microenvironment is uniquely poised to promote stem cell differentiation. Moreover, 2D cultures undergo minimal ECM remodeling and are ill-equipped to stimulate pathways associated with development. |
| format | Article |
| id | doaj-art-1bbcfc6576cc465687d2f2775e89d2cd |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-1bbcfc6576cc465687d2f2775e89d2cd2025-02-03T06:15:12ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/48096734809673Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted MicroenvironmentsQuyen A. Tran0Visar Ajeti1Brian T. Freeman2Paul J. Campagnola3Brenda M. Ogle4Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USADepartment of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USADepartment of Biomedical Engineering, University of Minnesota-Twin Cities, Minneapolis, MN 55455, USADepartment of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USADepartment of Biomedical Engineering, University of Minnesota-Twin Cities, Minneapolis, MN 55455, USADevelopmental studies and 3D in vitro model systems show that the production and engagement of extracellular matrix (ECM) often precede stem cell differentiation. Yet, unclear is how the ECM triggers signaling events in sequence to accommodate multistep process characteristic of differentiation. Here, we employ transcriptome profiling and advanced imaging to delineate the specificity of ECM engagement to particular differentiation pathways and to determine whether specificity in this context is a function of long-term ECM remodeling. To this end, human mesenchymal stem cells (hMSCs) were cultured in 3D bioprinted prisms created from ECM proteins and associated controls. We found that exogenous ECM provided in 3D microenvironments at early time points impacts on the composition of microenvironments at later time points and that each evolving 3D microenvironment is uniquely poised to promote stem cell differentiation. Moreover, 2D cultures undergo minimal ECM remodeling and are ill-equipped to stimulate pathways associated with development.http://dx.doi.org/10.1155/2018/4809673 |
| spellingShingle | Quyen A. Tran Visar Ajeti Brian T. Freeman Paul J. Campagnola Brenda M. Ogle Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments Stem Cells International |
| title | Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments |
| title_full | Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments |
| title_fullStr | Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments |
| title_full_unstemmed | Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments |
| title_short | Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments |
| title_sort | developmental pathways pervade stem cell responses to evolving extracellular matrices of 3d bioprinted microenvironments |
| url | http://dx.doi.org/10.1155/2018/4809673 |
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