Immune Gene Expression Profiling in Individuals with Turner Syndrome, Graves’ Disease, and a Healthy Female by Single-Cell RNA Sequencing: A Comparative Study
Turner syndrome (TS) can be determined by karyotype analysis, marked by the loss of one X chromosome in females. However, the genes involved in autoimmunity in TS patients remain unclear. In this study, we aimed to analyze differences in immune gene expression between a patient with TS, a healthy fe...
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2025-01-01
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| author | Soo Yeun Sim In-Cheol Baek Won Kyoung Cho Min Ho Jung Tai-Gyu Kim Byung-Kyu Suh |
| author_facet | Soo Yeun Sim In-Cheol Baek Won Kyoung Cho Min Ho Jung Tai-Gyu Kim Byung-Kyu Suh |
| author_sort | Soo Yeun Sim |
| collection | DOAJ |
| description | Turner syndrome (TS) can be determined by karyotype analysis, marked by the loss of one X chromosome in females. However, the genes involved in autoimmunity in TS patients remain unclear. In this study, we aimed to analyze differences in immune gene expression between a patient with TS, a healthy female, and a female patient with Graves’ disease using single-cell RNA sequencing (scRNA-seq) analysis of antigen-specific CD4(+) T cells. We identified 43 differentially expressed genes in the TS patient compared with the healthy female and the female patient with Graves’ disease. Many of these genes have previously been suggested to play a role in immune system regulation. This study provides valuable insights into the differences in immune-related gene expression between TS patients, healthy individuals, and those with autoimmune diseases. |
| format | Article |
| id | doaj-art-1bb5d6c008024119a0b4d41e306d9b49 |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj-art-1bb5d6c008024119a0b4d41e306d9b492025-01-24T13:26:39ZengMDPI AGCells2073-44092025-01-011429310.3390/cells14020093Immune Gene Expression Profiling in Individuals with Turner Syndrome, Graves’ Disease, and a Healthy Female by Single-Cell RNA Sequencing: A Comparative StudySoo Yeun Sim0In-Cheol Baek1Won Kyoung Cho2Min Ho Jung3Tai-Gyu Kim4Byung-Kyu Suh5Department of Pediatrics, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaCatholic Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Pediatrics, St. Vincent’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 16247, Republic of KoreaDepartment of Pediatrics, Yeouido St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 07345, Republic of KoreaDepartment of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Pediatrics, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaTurner syndrome (TS) can be determined by karyotype analysis, marked by the loss of one X chromosome in females. However, the genes involved in autoimmunity in TS patients remain unclear. In this study, we aimed to analyze differences in immune gene expression between a patient with TS, a healthy female, and a female patient with Graves’ disease using single-cell RNA sequencing (scRNA-seq) analysis of antigen-specific CD4(+) T cells. We identified 43 differentially expressed genes in the TS patient compared with the healthy female and the female patient with Graves’ disease. Many of these genes have previously been suggested to play a role in immune system regulation. This study provides valuable insights into the differences in immune-related gene expression between TS patients, healthy individuals, and those with autoimmune diseases.https://www.mdpi.com/2073-4409/14/2/93Turner syndromeX chromosome inactivationautoimmunitygene expressionsingle-cell RNA analysis |
| spellingShingle | Soo Yeun Sim In-Cheol Baek Won Kyoung Cho Min Ho Jung Tai-Gyu Kim Byung-Kyu Suh Immune Gene Expression Profiling in Individuals with Turner Syndrome, Graves’ Disease, and a Healthy Female by Single-Cell RNA Sequencing: A Comparative Study Cells Turner syndrome X chromosome inactivation autoimmunity gene expression single-cell RNA analysis |
| title | Immune Gene Expression Profiling in Individuals with Turner Syndrome, Graves’ Disease, and a Healthy Female by Single-Cell RNA Sequencing: A Comparative Study |
| title_full | Immune Gene Expression Profiling in Individuals with Turner Syndrome, Graves’ Disease, and a Healthy Female by Single-Cell RNA Sequencing: A Comparative Study |
| title_fullStr | Immune Gene Expression Profiling in Individuals with Turner Syndrome, Graves’ Disease, and a Healthy Female by Single-Cell RNA Sequencing: A Comparative Study |
| title_full_unstemmed | Immune Gene Expression Profiling in Individuals with Turner Syndrome, Graves’ Disease, and a Healthy Female by Single-Cell RNA Sequencing: A Comparative Study |
| title_short | Immune Gene Expression Profiling in Individuals with Turner Syndrome, Graves’ Disease, and a Healthy Female by Single-Cell RNA Sequencing: A Comparative Study |
| title_sort | immune gene expression profiling in individuals with turner syndrome graves disease and a healthy female by single cell rna sequencing a comparative study |
| topic | Turner syndrome X chromosome inactivation autoimmunity gene expression single-cell RNA analysis |
| url | https://www.mdpi.com/2073-4409/14/2/93 |
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