Investigation of the impact of ultraviolet B irradiation on apoptosis and proliferation in A375 melanoma cells
The increasing environmental impact of ultraviolet B (UVB) radiation, exacerbated by ozone layer depletion and anthropogenic influences, highlights its dual threat to ecological balance and human health. This study examines the cytotoxic mechanisms induced by UVB in A375 melanoma cells using dose-re...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2025-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/37/e3sconf_emer2025_02013.pdf |
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| author | Wang Jingyang Yuan Zhixiang Chen Taoyu Guo Jiaqi Qu Ying Hou Tao Zhu Lvyun Shao Tong |
| author_facet | Wang Jingyang Yuan Zhixiang Chen Taoyu Guo Jiaqi Qu Ying Hou Tao Zhu Lvyun Shao Tong |
| author_sort | Wang Jingyang |
| collection | DOAJ |
| description | The increasing environmental impact of ultraviolet B (UVB) radiation, exacerbated by ozone layer depletion and anthropogenic influences, highlights its dual threat to ecological balance and human health. This study examines the cytotoxic mechanisms induced by UVB in A375 melanoma cells using dose-response assays and multi-omics analysis. UVB exposure resulted in dose-dependent apoptosis and proliferation arrest, with irreversible cell cycle collapse observed at 48 hours. Transcriptomic analysis identified 303 differentially expressed genes, with p53 signaling emerging as the central regulator. UVB- activated p53 induced G2/M arrest through p21-mediated inhibition of CDK1/cyclin B, while simultaneous suppression of PI3K-AKT signaling and pyrimidine metabolism disrupted survival pathways and DNA repair, creating a self-reinforcing cytotoxic cascade. Notably, two previously uncharacterized genes were significantly downregulated, suggesting their potential roles in UVB stress adaptation. Functional validation confirmed the dysregulation of mitosis and adhesion pathways. These findings not only advance mechanistic insights into melanoma pathobiology but also propose actionable therapeutic strategies. Furthermore, the conserved stress responses identified here may inform ecological risk assessments, as UVB-driven molecular disruptions in melanoma cells mirror vulnerabilities observed in environmentally sensitive species like phytoplankton. |
| format | Article |
| id | doaj-art-1bad7b3fa75e4cfdbc1e9a9a47ea70ca |
| institution | DOAJ |
| issn | 2267-1242 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj-art-1bad7b3fa75e4cfdbc1e9a9a47ea70ca2025-08-20T03:12:46ZengEDP SciencesE3S Web of Conferences2267-12422025-01-016370201310.1051/e3sconf/202563702013e3sconf_emer2025_02013Investigation of the impact of ultraviolet B irradiation on apoptosis and proliferation in A375 melanoma cellsWang Jingyang0Yuan Zhixiang1Chen Taoyu2Guo Jiaqi3Qu Ying4Hou Tao5Zhu Lvyun6Shao Tong7Department of Biology and Chemistry, College of Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Sciences, National University of Defense TechnologyDepartment of Oncology, The Second Xiangya Hospital, Central South UniversityDepartment of Biology and Chemistry, College of Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Sciences, National University of Defense TechnologyThe increasing environmental impact of ultraviolet B (UVB) radiation, exacerbated by ozone layer depletion and anthropogenic influences, highlights its dual threat to ecological balance and human health. This study examines the cytotoxic mechanisms induced by UVB in A375 melanoma cells using dose-response assays and multi-omics analysis. UVB exposure resulted in dose-dependent apoptosis and proliferation arrest, with irreversible cell cycle collapse observed at 48 hours. Transcriptomic analysis identified 303 differentially expressed genes, with p53 signaling emerging as the central regulator. UVB- activated p53 induced G2/M arrest through p21-mediated inhibition of CDK1/cyclin B, while simultaneous suppression of PI3K-AKT signaling and pyrimidine metabolism disrupted survival pathways and DNA repair, creating a self-reinforcing cytotoxic cascade. Notably, two previously uncharacterized genes were significantly downregulated, suggesting their potential roles in UVB stress adaptation. Functional validation confirmed the dysregulation of mitosis and adhesion pathways. These findings not only advance mechanistic insights into melanoma pathobiology but also propose actionable therapeutic strategies. Furthermore, the conserved stress responses identified here may inform ecological risk assessments, as UVB-driven molecular disruptions in melanoma cells mirror vulnerabilities observed in environmentally sensitive species like phytoplankton.https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/37/e3sconf_emer2025_02013.pdf |
| spellingShingle | Wang Jingyang Yuan Zhixiang Chen Taoyu Guo Jiaqi Qu Ying Hou Tao Zhu Lvyun Shao Tong Investigation of the impact of ultraviolet B irradiation on apoptosis and proliferation in A375 melanoma cells E3S Web of Conferences |
| title | Investigation of the impact of ultraviolet B irradiation on apoptosis and proliferation in A375 melanoma cells |
| title_full | Investigation of the impact of ultraviolet B irradiation on apoptosis and proliferation in A375 melanoma cells |
| title_fullStr | Investigation of the impact of ultraviolet B irradiation on apoptosis and proliferation in A375 melanoma cells |
| title_full_unstemmed | Investigation of the impact of ultraviolet B irradiation on apoptosis and proliferation in A375 melanoma cells |
| title_short | Investigation of the impact of ultraviolet B irradiation on apoptosis and proliferation in A375 melanoma cells |
| title_sort | investigation of the impact of ultraviolet b irradiation on apoptosis and proliferation in a375 melanoma cells |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/37/e3sconf_emer2025_02013.pdf |
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