In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system
ObjectivesIrrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidi...
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Korean Academy of Conservative Dentistry
2019-02-01
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| Series: | Restorative Dentistry & Endodontics |
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| Online Access: | http://rde.ac/upload/pdf/rde-44-e8.pdf |
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| author | Jia Da Chum Darryl Jun Zhi Lim Sultan Omer Sheriff Shaju Jacob Pulikkotil Anand Suresh Fabian Davamani |
| author_facet | Jia Da Chum Darryl Jun Zhi Lim Sultan Omer Sheriff Shaju Jacob Pulikkotil Anand Suresh Fabian Davamani |
| author_sort | Jia Da Chum |
| collection | DOAJ |
| description | ObjectivesIrrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidis (S. epidermidis) for root canal disinfection.Materials and MethodsThe minimum inhibitory concentration (MIC) was obtained using serial dilution method. The agar diffusion method was then used to determine the zones of inhibition for each irrigant. Lastly, forty 6-mm dentin blocks were prepared from human mandibular premolars and inoculated with S. epidermidis. Samples were randomly divided into 4 groups of 10 blocks and irrigated for 3 minutes with saline (control), 2% CHX, 3% NaOCl, or 0.1% OCT. Dentin samples were then collected immediately for microbial analysis, including an analysis of colony-forming units (CFUs).ResultsThe MICs of each tested irrigant were 0.05% for CHX, 0.25% for NaOCl, and 0.0125% for OCT. All tested irrigants showed concentration-dependent increase in zones of inhibition, and 3% NaOCl showed the largest zone of inhibition amongst all tested irrigants (p < 0.05). There were no significant differences among the CFU measurements of 2% CHX, 3% NaOCl, and 0.1% OCT showing complete elimination of S. epidermidis in all samples.ConclusionsThis study showed that OCT was comparable to or even more effective than CHX and NaOCl, demonstrating antimicrobial activity at low concentrations against S. epidermidis. |
| format | Article |
| id | doaj-art-1ba96b41b156495f95278920ffc3cfe4 |
| institution | OA Journals |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2019-02-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-1ba96b41b156495f95278920ffc3cfe42025-08-20T02:38:30ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662019-02-01441e810.5395/rde.2019.44.e8887In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal systemJia Da Chum0Darryl Jun Zhi Lim1Sultan Omer Sheriff2Shaju Jacob Pulikkotil3Anand Suresh4Fabian Davamani5Department of Dental Surgery, School of Dentistry, International Medical University, Kuala Lumpur, MalaysiaDepartment of Dental Surgery, School of Dentistry, International Medical University, Kuala Lumpur, MalaysiaClinical Dentistry, School of Dentistry, International Medical University, Kuala Lumpur, MalaysiaClinical Dentistry, School of Dentistry, International Medical University, Kuala Lumpur, MalaysiaDepartment of Conservative Dentistry and Endodontics, Penang International Dental College, Penang, MalaysiaSchool of Health Sciences, International Medical University, Kuala Lumpur, MalaysiaObjectivesIrrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidis (S. epidermidis) for root canal disinfection.Materials and MethodsThe minimum inhibitory concentration (MIC) was obtained using serial dilution method. The agar diffusion method was then used to determine the zones of inhibition for each irrigant. Lastly, forty 6-mm dentin blocks were prepared from human mandibular premolars and inoculated with S. epidermidis. Samples were randomly divided into 4 groups of 10 blocks and irrigated for 3 minutes with saline (control), 2% CHX, 3% NaOCl, or 0.1% OCT. Dentin samples were then collected immediately for microbial analysis, including an analysis of colony-forming units (CFUs).ResultsThe MICs of each tested irrigant were 0.05% for CHX, 0.25% for NaOCl, and 0.0125% for OCT. All tested irrigants showed concentration-dependent increase in zones of inhibition, and 3% NaOCl showed the largest zone of inhibition amongst all tested irrigants (p < 0.05). There were no significant differences among the CFU measurements of 2% CHX, 3% NaOCl, and 0.1% OCT showing complete elimination of S. epidermidis in all samples.ConclusionsThis study showed that OCT was comparable to or even more effective than CHX and NaOCl, demonstrating antimicrobial activity at low concentrations against S. epidermidis.http://rde.ac/upload/pdf/rde-44-e8.pdfanti-bacterial agentschlorhexidineoctenidine dihydrochloridesodium hypochloritestaphylococcus epidermidis |
| spellingShingle | Jia Da Chum Darryl Jun Zhi Lim Sultan Omer Sheriff Shaju Jacob Pulikkotil Anand Suresh Fabian Davamani In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system Restorative Dentistry & Endodontics anti-bacterial agents chlorhexidine octenidine dihydrochloride sodium hypochlorite staphylococcus epidermidis |
| title | In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system |
| title_full | In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system |
| title_fullStr | In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system |
| title_full_unstemmed | In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system |
| title_short | In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system |
| title_sort | in vitro evaluation of octenidine as an antimicrobial agent against staphylococcus epidermidis in disinfecting the root canal system |
| topic | anti-bacterial agents chlorhexidine octenidine dihydrochloride sodium hypochlorite staphylococcus epidermidis |
| url | http://rde.ac/upload/pdf/rde-44-e8.pdf |
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