Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice
[Objective:] To explore the changes in condyle cartilage development and primary ciliary gene expression after changes in masticatory force. [Methods:] Three-week-old C57BL/6J male mice were divided into 3 groups, and they were fed with normal diet (ND) for 2 weeks, soft diet (SD) for 2 weeks, and f...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2024-10-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2024.05.002 |
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| _version_ | 1849225005198475264 |
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| author | HU Ziyue ZHANG Shuai SUN Yao |
| author_facet | HU Ziyue ZHANG Shuai SUN Yao |
| author_sort | HU Ziyue |
| collection | DOAJ |
| description | [Objective:] To explore the changes in condyle cartilage development and primary ciliary gene expression after changes in masticatory force. [Methods:] Three-week-old C57BL/6J male mice were divided into 3 groups, and they were fed with normal diet (ND) for 2 weeks, soft diet (SD) for 2 weeks, and fed with 1 week of soft diet than 1 week of re-normal diet (RND). After collecting the samples, safranin O-fast green staining, toluidine blue staining, Masson staining, alkaline phosphatase (ALP) staining, and collagen typeⅡ(COL2) immunofluorescence staining were performed to observe changes in the condylar cartilage and subchondral bone. Primary ciliary gene expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR). [Results:] Compared with the ND group, the SD group has thinner condyle cartilage, less extracellular matrix, and subchondral bone structure has changed. The primary cilia gene expression (Ift88, Ift140, Ptch1, Smo, Gli1) was decreased. Compared with the SD group, the above changes recovered in the RND group. [Conclusion:] Changes of masticatory force during growth will affect the development of condyle cartilage and primary ciliary gene expression in mice. |
| format | Article |
| id | doaj-art-1ba7f098f4484052b32a7eb563f786d7 |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2024-10-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-1ba7f098f4484052b32a7eb563f786d72025-08-25T06:11:20ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792024-10-0134533634110.12439/kqhm.1005-4979.2024.05.002Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in miceHU Ziyue0ZHANG Shuai1SUN Yao2Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Oral Implantology, Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Oral Implantology, Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Oral Implantology, Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China[Objective:] To explore the changes in condyle cartilage development and primary ciliary gene expression after changes in masticatory force. [Methods:] Three-week-old C57BL/6J male mice were divided into 3 groups, and they were fed with normal diet (ND) for 2 weeks, soft diet (SD) for 2 weeks, and fed with 1 week of soft diet than 1 week of re-normal diet (RND). After collecting the samples, safranin O-fast green staining, toluidine blue staining, Masson staining, alkaline phosphatase (ALP) staining, and collagen typeⅡ(COL2) immunofluorescence staining were performed to observe changes in the condylar cartilage and subchondral bone. Primary ciliary gene expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR). [Results:] Compared with the ND group, the SD group has thinner condyle cartilage, less extracellular matrix, and subchondral bone structure has changed. The primary cilia gene expression (Ift88, Ift140, Ptch1, Smo, Gli1) was decreased. Compared with the SD group, the above changes recovered in the RND group. [Conclusion:] Changes of masticatory force during growth will affect the development of condyle cartilage and primary ciliary gene expression in mice.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2024.05.002condyle cartilagemasticatory forceprimary cilia |
| spellingShingle | HU Ziyue ZHANG Shuai SUN Yao Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice Kouqiang hemian waike zazhi condyle cartilage masticatory force primary cilia |
| title | Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice |
| title_full | Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice |
| title_fullStr | Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice |
| title_full_unstemmed | Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice |
| title_short | Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice |
| title_sort | changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice |
| topic | condyle cartilage masticatory force primary cilia |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2024.05.002 |
| work_keys_str_mv | AT huziyue changesofmasticatoryforceaffectscondylecartilagedevelopmentandprimaryciliarygeneexpressioninmice AT zhangshuai changesofmasticatoryforceaffectscondylecartilagedevelopmentandprimaryciliarygeneexpressioninmice AT sunyao changesofmasticatoryforceaffectscondylecartilagedevelopmentandprimaryciliarygeneexpressioninmice |