A comprehensive genome-wide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum.
Productivity of wheat crop is largely dependent on its growth and development that, in turn, is mainly regulated by environmental conditions, including abiotic stress factors. miRNAs are key regulators of gene expression networks involved in diverse aspects of development and stress responses in pla...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095800&type=printable |
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| author | Ritu Pandey Gopal Joshi Ankur R Bhardwaj Manu Agarwal Surekha Katiyar-Agarwal |
| author_facet | Ritu Pandey Gopal Joshi Ankur R Bhardwaj Manu Agarwal Surekha Katiyar-Agarwal |
| author_sort | Ritu Pandey |
| collection | DOAJ |
| description | Productivity of wheat crop is largely dependent on its growth and development that, in turn, is mainly regulated by environmental conditions, including abiotic stress factors. miRNAs are key regulators of gene expression networks involved in diverse aspects of development and stress responses in plants. Using high-throughput sequencing of eight small RNA libraries prepared from diverse abiotic stresses and tissues, we identified 47 known miRNAs belonging to 20 families, 49 true novel and 1030 candidate novel miRNAs. Digital gene expression analysis revealed that 257 miRNAs exhibited tissue-specific expression and 74 were associated with abiotic stresses. Putative target genes were predicted for miRNAs identified in this study and their grouping into functional categories indicated that the putative targets were involved in diverse biological processes. RLM-RACE of predicted targets of three known miRNAs (miR156, miR160 and miR164) confirmed their mRNA cleavage, thus indicating their regulation at post-transcriptional level by the corresponding miRNAs. Mapping of the sequenced data onto the wheat progenitors and closely related monocots revealed a large number of evolutionary conserved miRNAs. Additional expression profiling of some of these miRNAs in other abiotic stresses underline their involvement in multiple stresses. Our findings provide valuable resource for an improved understanding of the role of miRNAs in stress tolerance as well as plant development. |
| format | Article |
| id | doaj-art-1b99f9b01c044bd286fda7634e3d1278 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-1b99f9b01c044bd286fda7634e3d12782025-08-20T02:14:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9580010.1371/journal.pone.0095800A comprehensive genome-wide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum.Ritu PandeyGopal JoshiAnkur R BhardwajManu AgarwalSurekha Katiyar-AgarwalProductivity of wheat crop is largely dependent on its growth and development that, in turn, is mainly regulated by environmental conditions, including abiotic stress factors. miRNAs are key regulators of gene expression networks involved in diverse aspects of development and stress responses in plants. Using high-throughput sequencing of eight small RNA libraries prepared from diverse abiotic stresses and tissues, we identified 47 known miRNAs belonging to 20 families, 49 true novel and 1030 candidate novel miRNAs. Digital gene expression analysis revealed that 257 miRNAs exhibited tissue-specific expression and 74 were associated with abiotic stresses. Putative target genes were predicted for miRNAs identified in this study and their grouping into functional categories indicated that the putative targets were involved in diverse biological processes. RLM-RACE of predicted targets of three known miRNAs (miR156, miR160 and miR164) confirmed their mRNA cleavage, thus indicating their regulation at post-transcriptional level by the corresponding miRNAs. Mapping of the sequenced data onto the wheat progenitors and closely related monocots revealed a large number of evolutionary conserved miRNAs. Additional expression profiling of some of these miRNAs in other abiotic stresses underline their involvement in multiple stresses. Our findings provide valuable resource for an improved understanding of the role of miRNAs in stress tolerance as well as plant development.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095800&type=printable |
| spellingShingle | Ritu Pandey Gopal Joshi Ankur R Bhardwaj Manu Agarwal Surekha Katiyar-Agarwal A comprehensive genome-wide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum. PLoS ONE |
| title | A comprehensive genome-wide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum. |
| title_full | A comprehensive genome-wide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum. |
| title_fullStr | A comprehensive genome-wide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum. |
| title_full_unstemmed | A comprehensive genome-wide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum. |
| title_short | A comprehensive genome-wide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum. |
| title_sort | comprehensive genome wide study on tissue specific and abiotic stress specific mirnas in triticum aestivum |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095800&type=printable |
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