Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management
Abstract The healing of bacterial biofilm‐infected wounds is a complex process, and the construction of emerging therapeutic modalities that regulate the microenvironment to magnify therapeutic effects and reduce biotoxicity is still highly challenging. Herein, an engineered microneedle (MN) patch i...
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| Format: | Article |
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Wiley
2025-03-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202412226 |
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| author | Shiyang Lin Zhongqi Cui Qingqiong Luo Chen Li Yue Zhang Fengjiao Yang Yichuan Chen Chuansheng Xu Yan Gao Shasha Zhao Fenyong Sun Dandan Shen Qi Wu Shuo Shi |
| author_facet | Shiyang Lin Zhongqi Cui Qingqiong Luo Chen Li Yue Zhang Fengjiao Yang Yichuan Chen Chuansheng Xu Yan Gao Shasha Zhao Fenyong Sun Dandan Shen Qi Wu Shuo Shi |
| author_sort | Shiyang Lin |
| collection | DOAJ |
| description | Abstract The healing of bacterial biofilm‐infected wounds is a complex process, and the construction of emerging therapeutic modalities that regulate the microenvironment to magnify therapeutic effects and reduce biotoxicity is still highly challenging. Herein, an engineered microneedle (MN) patch is reported to mediate the efficient delivery of black phosphorus nanosheets (BP NSs) and copper peroxide nanodots (CP NDs) for dual nanodynamic sterilization and methicillin‐resistant staphylococcus aureus (MRSA)‐infected wound healing. Results demonstrate that the system can eliminate biofilm, reduce cytotoxicity, promote angiogenesis and tissue regeneration by the multiple advantages of chemodynamic therapy (CDT), enhanced photodynamic therapy (PDT), and improved degradation process from BP NSs to phosphate for promoting cell proliferation. Notably, the balance between excellent photodynamic stability and rapid degradability of BP NSs is maintained, and the improved degradation mechanism of BP NSs is vividly elucidated by density functional theory (DFT)‐based molecular dynamics (MD) calculations. Furthermore, the transcriptional changes of treated MRSA‐infected skin are studied using RNA‐seq technology to reveal the potential therapeutic mechanism. As envisaged, the proposed MN patch provides a safe, easy, also highly effective approach to achieve the temporal regulation of sterilization and tissue regeneration for bacterial biofilm‐infected wounds. |
| format | Article |
| id | doaj-art-1b8e896b22a54c06a3c3c5de0ef4083d |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-1b8e896b22a54c06a3c3c5de0ef4083d2025-08-20T03:32:24ZengWileyAdvanced Science2198-38442025-03-01129n/an/a10.1002/advs.202412226Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound ManagementShiyang Lin0Zhongqi Cui1Qingqiong Luo2Chen Li3Yue Zhang4Fengjiao Yang5Yichuan Chen6Chuansheng Xu7Yan Gao8Shasha Zhao9Fenyong Sun10Dandan Shen11Qi Wu12Shuo Shi13Department of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Clinical Laboratory Medicine Shanghai Skin Disease Hospital, School of Medicine Tongji University Shanghai 200443 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaTongji University School of Medicine Tongji University Shanghai 200092 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaDepartment of Clinical Laboratory Shanghai Children's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200062 P. R. ChinaDepartment of Laboratory Medicine School of Chemical Science and Engineering Shanghai Tenth People's Hospital of Tongji University Tongji University Shanghai 200092 P. R. ChinaAbstract The healing of bacterial biofilm‐infected wounds is a complex process, and the construction of emerging therapeutic modalities that regulate the microenvironment to magnify therapeutic effects and reduce biotoxicity is still highly challenging. Herein, an engineered microneedle (MN) patch is reported to mediate the efficient delivery of black phosphorus nanosheets (BP NSs) and copper peroxide nanodots (CP NDs) for dual nanodynamic sterilization and methicillin‐resistant staphylococcus aureus (MRSA)‐infected wound healing. Results demonstrate that the system can eliminate biofilm, reduce cytotoxicity, promote angiogenesis and tissue regeneration by the multiple advantages of chemodynamic therapy (CDT), enhanced photodynamic therapy (PDT), and improved degradation process from BP NSs to phosphate for promoting cell proliferation. Notably, the balance between excellent photodynamic stability and rapid degradability of BP NSs is maintained, and the improved degradation mechanism of BP NSs is vividly elucidated by density functional theory (DFT)‐based molecular dynamics (MD) calculations. Furthermore, the transcriptional changes of treated MRSA‐infected skin are studied using RNA‐seq technology to reveal the potential therapeutic mechanism. As envisaged, the proposed MN patch provides a safe, easy, also highly effective approach to achieve the temporal regulation of sterilization and tissue regeneration for bacterial biofilm‐infected wounds.https://doi.org/10.1002/advs.202412226black phosphorus nanosheetscopper peroxide nanodotsengineered microneedle patchnanodynamic sterilizationwound healing |
| spellingShingle | Shiyang Lin Zhongqi Cui Qingqiong Luo Chen Li Yue Zhang Fengjiao Yang Yichuan Chen Chuansheng Xu Yan Gao Shasha Zhao Fenyong Sun Dandan Shen Qi Wu Shuo Shi Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management Advanced Science black phosphorus nanosheets copper peroxide nanodots engineered microneedle patch nanodynamic sterilization wound healing |
| title | Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management |
| title_full | Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management |
| title_fullStr | Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management |
| title_full_unstemmed | Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management |
| title_short | Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management |
| title_sort | engineered microneedle system enables the smart regulation of nanodynamic sterilization and tissue regeneration for wound management |
| topic | black phosphorus nanosheets copper peroxide nanodots engineered microneedle patch nanodynamic sterilization wound healing |
| url | https://doi.org/10.1002/advs.202412226 |
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