Study on the effect of modified clay on algae-derived organic nitrogen mineralization and its mechanisms in diatom Skeletonema costatum

Algae-derived organic nitrogen (AON) is mineralized by microorganisms to bioavailable inorganic nitrogen form, potentially sustaining the harmful algal blooms (HABs) for extended durations. The modified clay (MC) is an effective approach for mitigating HABs; however, its effects on the AON mineraliz...

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Main Authors: Xin Feng, Wentao Wang, Yongbao Chu, Jianan Zhu, Lianbao Chi, Jing Chen, Xiuxian Song, Zhiming Yu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Marine Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fmars.2025.1558899/full
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author Xin Feng
Wentao Wang
Wentao Wang
Wentao Wang
Yongbao Chu
Jianan Zhu
Jianan Zhu
Lianbao Chi
Lianbao Chi
Jing Chen
Jing Chen
Jing Chen
Xiuxian Song
Xiuxian Song
Xiuxian Song
Zhiming Yu
Zhiming Yu
Zhiming Yu
author_facet Xin Feng
Wentao Wang
Wentao Wang
Wentao Wang
Yongbao Chu
Jianan Zhu
Jianan Zhu
Lianbao Chi
Lianbao Chi
Jing Chen
Jing Chen
Jing Chen
Xiuxian Song
Xiuxian Song
Xiuxian Song
Zhiming Yu
Zhiming Yu
Zhiming Yu
author_sort Xin Feng
collection DOAJ
description Algae-derived organic nitrogen (AON) is mineralized by microorganisms to bioavailable inorganic nitrogen form, potentially sustaining the harmful algal blooms (HABs) for extended durations. The modified clay (MC) is an effective approach for mitigating HABs; however, its effects on the AON mineralization and the underlying mechanism remain unclear. In this study, the effects of MC on the mineralization of AON by a typical HAB species Skeletonema costatum were analyzed using the isotope dilution method, and the underlying mechanism were preliminarily discussed. The results revealed that the addition of MC could reduce the mineralization rates of AON and the regeneration amount of inorganic nitrogen by 71% and 60%, respectively, compared to the control group. The total fluorescence intensity was approximately 46% lower than that of the control group. In addition, the bacterial proliferation was suppressed by 66% and the community evolved from uniformity to diversification. It is hypothesized that the physical encapsulation of AON, chemical bonding of molecules and the variations of the microbial community under the utilization of MC were key mechanisms influencing the mineralization process. This study offers valuable insights into the environmental impacts following the HABs management and provides a scientific basis for investigating the controlling mechanisms of marine organic matter mineralization and burial.
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publisher Frontiers Media S.A.
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spelling doaj-art-1b63fc92a64f4b9caabbc0fdad4f0b2b2025-08-20T01:51:53ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-04-011210.3389/fmars.2025.15588991558899Study on the effect of modified clay on algae-derived organic nitrogen mineralization and its mechanisms in diatom Skeletonema costatumXin Feng0Wentao Wang1Wentao Wang2Wentao Wang3Yongbao Chu4Jianan Zhu5Jianan Zhu6Lianbao Chi7Lianbao Chi8Jing Chen9Jing Chen10Jing Chen11Xiuxian Song12Xiuxian Song13Xiuxian Song14Zhiming Yu15Zhiming Yu16Zhiming Yu17School of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, ChinaCollege of Marine Sciences, University of Chinese Academy of Sciences, Beijing, ChinaSchool of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, ChinaCollege of Marine Sciences, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, ChinaCollege of Marine Sciences, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, ChinaCollege of Marine Sciences, University of Chinese Academy of Sciences, Beijing, ChinaAlgae-derived organic nitrogen (AON) is mineralized by microorganisms to bioavailable inorganic nitrogen form, potentially sustaining the harmful algal blooms (HABs) for extended durations. The modified clay (MC) is an effective approach for mitigating HABs; however, its effects on the AON mineralization and the underlying mechanism remain unclear. In this study, the effects of MC on the mineralization of AON by a typical HAB species Skeletonema costatum were analyzed using the isotope dilution method, and the underlying mechanism were preliminarily discussed. The results revealed that the addition of MC could reduce the mineralization rates of AON and the regeneration amount of inorganic nitrogen by 71% and 60%, respectively, compared to the control group. The total fluorescence intensity was approximately 46% lower than that of the control group. In addition, the bacterial proliferation was suppressed by 66% and the community evolved from uniformity to diversification. It is hypothesized that the physical encapsulation of AON, chemical bonding of molecules and the variations of the microbial community under the utilization of MC were key mechanisms influencing the mineralization process. This study offers valuable insights into the environmental impacts following the HABs management and provides a scientific basis for investigating the controlling mechanisms of marine organic matter mineralization and burial.https://www.frontiersin.org/articles/10.3389/fmars.2025.1558899/fullmodified clayskeletonema costatumalgae-derived organic nitrogennitrogen mineralizationbacterial community
spellingShingle Xin Feng
Wentao Wang
Wentao Wang
Wentao Wang
Yongbao Chu
Jianan Zhu
Jianan Zhu
Lianbao Chi
Lianbao Chi
Jing Chen
Jing Chen
Jing Chen
Xiuxian Song
Xiuxian Song
Xiuxian Song
Zhiming Yu
Zhiming Yu
Zhiming Yu
Study on the effect of modified clay on algae-derived organic nitrogen mineralization and its mechanisms in diatom Skeletonema costatum
Frontiers in Marine Science
modified clay
skeletonema costatum
algae-derived organic nitrogen
nitrogen mineralization
bacterial community
title Study on the effect of modified clay on algae-derived organic nitrogen mineralization and its mechanisms in diatom Skeletonema costatum
title_full Study on the effect of modified clay on algae-derived organic nitrogen mineralization and its mechanisms in diatom Skeletonema costatum
title_fullStr Study on the effect of modified clay on algae-derived organic nitrogen mineralization and its mechanisms in diatom Skeletonema costatum
title_full_unstemmed Study on the effect of modified clay on algae-derived organic nitrogen mineralization and its mechanisms in diatom Skeletonema costatum
title_short Study on the effect of modified clay on algae-derived organic nitrogen mineralization and its mechanisms in diatom Skeletonema costatum
title_sort study on the effect of modified clay on algae derived organic nitrogen mineralization and its mechanisms in diatom skeletonema costatum
topic modified clay
skeletonema costatum
algae-derived organic nitrogen
nitrogen mineralization
bacterial community
url https://www.frontiersin.org/articles/10.3389/fmars.2025.1558899/full
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