Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion
Objective: To compare the surface properties of self-ligating metallic (SLM), ceramic esthetic, and conventional metallic (CM) brackets, and evaluate the adhesion of Streptococcus mutans biofilms to their surface, attempting to interpret the correlation between bracket type and enamel demineralizati...
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| Format: | Article |
| Language: | English |
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Springer
2022-05-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905222000475 |
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| author | Raphaella Barcellos Fernandes Ana Bárbara Polo Vinicius Novaes Rocha Robert Willer Farinazzo Vitral Ana Carolina Morais Apolônio Marcio José da Silva Campos |
| author_facet | Raphaella Barcellos Fernandes Ana Bárbara Polo Vinicius Novaes Rocha Robert Willer Farinazzo Vitral Ana Carolina Morais Apolônio Marcio José da Silva Campos |
| author_sort | Raphaella Barcellos Fernandes |
| collection | DOAJ |
| description | Objective: To compare the surface properties of self-ligating metallic (SLM), ceramic esthetic, and conventional metallic (CM) brackets, and evaluate the adhesion of Streptococcus mutans biofilms to their surface, attempting to interpret the correlation between bracket type and enamel demineralization from a microbiological perspective. Materials and methods: Twenty-two brackets of each group were used. The brackets’ surface roughness was defined and the bacterial adhesion was performed using the strain S. mutans ATCC25175 with 8 h or 24 h of incubation time. The total bacterial adhesion (TBA) of biofilms was assessed using optical density (OD) methodology. To quantify bacteria viability (BV), the colony forming units (CFU) were counted. A scanning electron microscopy (SEM) observation of biofilms was also performed. Results: Ceramic brackets exhibited significantly higher roughness (0.304) compared to CM (0.090) and SLM (0.067) ones (C > CM = SLM). The data obtained with the TBA and BV tests showed that S. mutans biofilm formed on bracket groups exhibited similar results for both incubation periods. From the SEM images it is possible to observe that biofilm structure formed for 24 h was denser than that for 8 h of incubation with significantly more aggregates and cells for three groups. Conclusion: This in vitro study suggests that despite the higher surface roughness of ceramic brackets, this alone does not influence the adhesion of the S. mutans biofilms. Clinical relevance: From a microbiological perspective, the bracket's design may be more relevant than its surface roughness with respect to the adhesion of cariogenic bacteria biofilm with potential risk to dental enamel integrity. |
| format | Article |
| id | doaj-art-1b5dcd2c2345453e80f0b59c4fd14ba0 |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2022-05-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-1b5dcd2c2345453e80f0b59c4fd14ba02025-08-20T02:02:24ZengSpringerSaudi Dental Journal1013-90522022-05-0134432132710.1016/j.sdentj.2022.03.008Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesionRaphaella Barcellos Fernandes0Ana Bárbara Polo1Vinicius Novaes Rocha2Robert Willer Farinazzo Vitral3Ana Carolina Morais Apolônio4Marcio José da Silva Campos5Graduate Program in Dentistry, Federal University of Juiz de Fora, Juiz de Fora, BrazilGraduate Program in Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, BrazilDepartment of Veterinary Medicine, Federal University of Juiz de Fora, Juiz de Fora, BrazilGraduate Program in Dentistry, Federal University of Juiz de Fora, Juiz de Fora, BrazilGraduate Program in Dentistry and Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Brazil; Corresponding author at: Department of Parasitology, Microbiology, and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36.036-900 Juiz de Fora, MG, Brazil.Graduate Program in Dentistry, Federal University of Juiz de Fora, Juiz de Fora, BrazilObjective: To compare the surface properties of self-ligating metallic (SLM), ceramic esthetic, and conventional metallic (CM) brackets, and evaluate the adhesion of Streptococcus mutans biofilms to their surface, attempting to interpret the correlation between bracket type and enamel demineralization from a microbiological perspective. Materials and methods: Twenty-two brackets of each group were used. The brackets’ surface roughness was defined and the bacterial adhesion was performed using the strain S. mutans ATCC25175 with 8 h or 24 h of incubation time. The total bacterial adhesion (TBA) of biofilms was assessed using optical density (OD) methodology. To quantify bacteria viability (BV), the colony forming units (CFU) were counted. A scanning electron microscopy (SEM) observation of biofilms was also performed. Results: Ceramic brackets exhibited significantly higher roughness (0.304) compared to CM (0.090) and SLM (0.067) ones (C > CM = SLM). The data obtained with the TBA and BV tests showed that S. mutans biofilm formed on bracket groups exhibited similar results for both incubation periods. From the SEM images it is possible to observe that biofilm structure formed for 24 h was denser than that for 8 h of incubation with significantly more aggregates and cells for three groups. Conclusion: This in vitro study suggests that despite the higher surface roughness of ceramic brackets, this alone does not influence the adhesion of the S. mutans biofilms. Clinical relevance: From a microbiological perspective, the bracket's design may be more relevant than its surface roughness with respect to the adhesion of cariogenic bacteria biofilm with potential risk to dental enamel integrity.http://www.sciencedirect.com/science/article/pii/S1013905222000475Orthodontic bracketsBacterial adhesionStreptococcus mutansSurface PropertiesBiofilm |
| spellingShingle | Raphaella Barcellos Fernandes Ana Bárbara Polo Vinicius Novaes Rocha Robert Willer Farinazzo Vitral Ana Carolina Morais Apolônio Marcio José da Silva Campos Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion Saudi Dental Journal Orthodontic brackets Bacterial adhesion Streptococcus mutans Surface Properties Biofilm |
| title | Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion |
| title_full | Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion |
| title_fullStr | Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion |
| title_full_unstemmed | Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion |
| title_short | Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion |
| title_sort | influence of orthodontic brackets design and surface properties on the cariogenic streptococcus mutans adhesion |
| topic | Orthodontic brackets Bacterial adhesion Streptococcus mutans Surface Properties Biofilm |
| url | http://www.sciencedirect.com/science/article/pii/S1013905222000475 |
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