Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i>
This study presents the optimal light spectrum and medium composition for the in vitro induction of adventitious bulbs in <i>T. tarda</i> Stapf. Bulb scales, used as explants, were cultivated on 100% MS solid media with 3% or 6% sucrose and 0 or 0.5 µM BAP (6-benzyl-aminopurine) under a...
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2025-03-01
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| author | Małgorzata Maślanka |
| author_facet | Małgorzata Maślanka |
| author_sort | Małgorzata Maślanka |
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| description | This study presents the optimal light spectrum and medium composition for the in vitro induction of adventitious bulbs in <i>T. tarda</i> Stapf. Bulb scales, used as explants, were cultivated on 100% MS solid media with 3% or 6% sucrose and 0 or 0.5 µM BAP (6-benzyl-aminopurine) under a 16 h photoperiod and different fluorescent light treatments (white, red, blue). Darkness was used as a control. The cultures were maintained at 20 ± 2 °C for 12 weeks. The obtained results revealed that white light combined with 6% sucrose yielded the highest adventitious bulb formation, with an average of 12.1 ± 1.3 bulbs per explant. The bulbs formed directly on the surface of the explants. Red light combined with 3% sucrose and 0.5 µM BAP completely inhibited bulb formation, while darkness promoted leaf development, with a maximum of 1.3 ± 0.1 leaves per bulb, under darkness on medium with 6% sucrose. The addition of BAP, in most cases, was essential for root formation, with a maximum of 2.9 ± 0.6 roots per bulb under the influence of white light. This study demonstrates that it is possible to obtain an effective and efficient method for <i>T. tarda</i> propagation from bulb scales treated with 6% sucrose under white light condition. This method offers the potential for the commercial cultivation of this ornamental species. |
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| id | doaj-art-1b5c26e9f00a409b95ec80eacab24a39 |
| institution | DOAJ |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-1b5c26e9f00a409b95ec80eacab24a392025-08-20T02:41:58ZengMDPI AGAgronomy2073-43952025-03-0115364210.3390/agronomy15030642Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i>Małgorzata Maślanka0Department of Ornamental Plants and Garden Art, University of Agriculture in Kraków, al. 29 Listopada 54, 31-425 Kraków, PolandThis study presents the optimal light spectrum and medium composition for the in vitro induction of adventitious bulbs in <i>T. tarda</i> Stapf. Bulb scales, used as explants, were cultivated on 100% MS solid media with 3% or 6% sucrose and 0 or 0.5 µM BAP (6-benzyl-aminopurine) under a 16 h photoperiod and different fluorescent light treatments (white, red, blue). Darkness was used as a control. The cultures were maintained at 20 ± 2 °C for 12 weeks. The obtained results revealed that white light combined with 6% sucrose yielded the highest adventitious bulb formation, with an average of 12.1 ± 1.3 bulbs per explant. The bulbs formed directly on the surface of the explants. Red light combined with 3% sucrose and 0.5 µM BAP completely inhibited bulb formation, while darkness promoted leaf development, with a maximum of 1.3 ± 0.1 leaves per bulb, under darkness on medium with 6% sucrose. The addition of BAP, in most cases, was essential for root formation, with a maximum of 2.9 ± 0.6 roots per bulb under the influence of white light. This study demonstrates that it is possible to obtain an effective and efficient method for <i>T. tarda</i> propagation from bulb scales treated with 6% sucrose under white light condition. This method offers the potential for the commercial cultivation of this ornamental species.https://www.mdpi.com/2073-4395/15/3/642micropropagationbulbingbulb scale explantBAPsucrose |
| spellingShingle | Małgorzata Maślanka Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i> Agronomy micropropagation bulbing bulb scale explant BAP sucrose |
| title | Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i> |
| title_full | Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i> |
| title_fullStr | Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i> |
| title_full_unstemmed | Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i> |
| title_short | Effect of Light Spectrum, Sucrose Concentration, and 6-Benzyl-aminopurine on In Vitro Adventitious Bulb Formation in <i>Tulipa tarda</i> |
| title_sort | effect of light spectrum sucrose concentration and 6 benzyl aminopurine on in vitro adventitious bulb formation in i tulipa tarda i |
| topic | micropropagation bulbing bulb scale explant BAP sucrose |
| url | https://www.mdpi.com/2073-4395/15/3/642 |
| work_keys_str_mv | AT małgorzatamaslanka effectoflightspectrumsucroseconcentrationand6benzylaminopurineoninvitroadventitiousbulbformationinitulipatardai |