Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala)
Abstract Background The blunt snout bream (Megalobrama amblycephala) is an important economic freshwater fish in China with tender flesh and high nutritional value. With the cultivation of superior new varieties and the expansion of breeding scale, it becomes imperative to employ sex-control technol...
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2024-10-01
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| Online Access: | https://doi.org/10.1186/s12864-024-10884-0 |
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| author | Yuye Fu Lifei Luo Shilong Wang Yue Yu Yao Wang Zexia Gao |
| author_facet | Yuye Fu Lifei Luo Shilong Wang Yue Yu Yao Wang Zexia Gao |
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| description | Abstract Background The blunt snout bream (Megalobrama amblycephala) is an important economic freshwater fish in China with tender flesh and high nutritional value. With the cultivation of superior new varieties and the expansion of breeding scale, it becomes imperative to employ sex-control technology to cultivate monosexual populations of M. amblycephala, thereby preventing the deterioration of desirable traits. The development of specific markers capable of accurately identifying the sex of M. amblycephala would facilitate the determination of the genetic sex of the breeding population before gonad maturation, thereby expediting the processes of sex-controlled breeding of M. amblycephala. Results A whole-genome re-sequencing was performed for 116 females and 141 males M. amblycephala collected from nine populations. Seven candidate male-specific sequences were identified through comparative analysis of male and female genomes, which were further compared with the sequencing data of 257 individuals, and finally three male-specific sequences were generated. These three sequences were further validated by PCR amplification in 32 males and 32 females to confirm their potential as male-specific molecular markers for M. amblycephala. One of these markers showed potential applicability in M. pellegrini as well, enabling males to be identified using this specific molecular marker. Conclusions The study provides a high-efficiency and cost-effective approach for the genetic sex identification in two species of Megalobrama. The developed markers in this study have great potential in facilitating sex-controlled breeding of M. amblycephala and M. pellegrini, while also contributing valuable insights into the underlying mechanisms of fish sex determination. |
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| institution | OA Journals |
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| language | English |
| publishDate | 2024-10-01 |
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| spelling | doaj-art-1b5b6cb7e4fc43f89e88baff287c5d322025-08-20T02:17:39ZengBMCBMC Genomics1471-21642024-10-0125111210.1186/s12864-024-10884-0Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala)Yuye Fu0Lifei Luo1Shilong Wang2Yue Yu3Yao Wang4Zexia Gao5College of Fisheries, Hubei Hongshan Laboratory / Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs / Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural UniversityCollege of Fisheries, Hubei Hongshan Laboratory / Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs / Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural UniversityCollege of Fisheries, Hubei Hongshan Laboratory / Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs / Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural UniversityCollege of Fisheries, Hubei Hongshan Laboratory / Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs / Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural UniversityCollege of Fisheries, Hubei Hongshan Laboratory / Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs / Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural UniversityCollege of Fisheries, Hubei Hongshan Laboratory / Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs / Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural UniversityAbstract Background The blunt snout bream (Megalobrama amblycephala) is an important economic freshwater fish in China with tender flesh and high nutritional value. With the cultivation of superior new varieties and the expansion of breeding scale, it becomes imperative to employ sex-control technology to cultivate monosexual populations of M. amblycephala, thereby preventing the deterioration of desirable traits. The development of specific markers capable of accurately identifying the sex of M. amblycephala would facilitate the determination of the genetic sex of the breeding population before gonad maturation, thereby expediting the processes of sex-controlled breeding of M. amblycephala. Results A whole-genome re-sequencing was performed for 116 females and 141 males M. amblycephala collected from nine populations. Seven candidate male-specific sequences were identified through comparative analysis of male and female genomes, which were further compared with the sequencing data of 257 individuals, and finally three male-specific sequences were generated. These three sequences were further validated by PCR amplification in 32 males and 32 females to confirm their potential as male-specific molecular markers for M. amblycephala. One of these markers showed potential applicability in M. pellegrini as well, enabling males to be identified using this specific molecular marker. Conclusions The study provides a high-efficiency and cost-effective approach for the genetic sex identification in two species of Megalobrama. The developed markers in this study have great potential in facilitating sex-controlled breeding of M. amblycephala and M. pellegrini, while also contributing valuable insights into the underlying mechanisms of fish sex determination.https://doi.org/10.1186/s12864-024-10884-0Megalobrama amblycephalaWhole-genome re-sequencingGenetic sex identificationMolecular marker |
| spellingShingle | Yuye Fu Lifei Luo Shilong Wang Yue Yu Yao Wang Zexia Gao Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala) BMC Genomics Megalobrama amblycephala Whole-genome re-sequencing Genetic sex identification Molecular marker |
| title | Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala) |
| title_full | Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala) |
| title_fullStr | Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala) |
| title_full_unstemmed | Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala) |
| title_short | Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala) |
| title_sort | identification of sex specific markers using genome re sequencing in the blunt snout bream megalobrama amblycephala |
| topic | Megalobrama amblycephala Whole-genome re-sequencing Genetic sex identification Molecular marker |
| url | https://doi.org/10.1186/s12864-024-10884-0 |
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