CircVAMP3 promotes acute lung injury progression by regulating the miR-580-3p/STING/IFN-β axis in macrophages

Circular RNA (circRNA) is a prominent group of non-coding RNA (ncRNA) that regulates gene expression at the post-transcriptional level in various diseases including acute respiratory distress syndrome (ARDS). In our study, circVAMP3 was a novel circRNA significantly increased in macrophages in an ac...

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Main Authors: Zheng Zhou, Shuyang Chen, Yajun Wang, Shujing Chen, Jinjun Jiang
Format: Article
Language:English
Published: PeerJ Inc. 2025-06-01
Series:PeerJ
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Online Access:https://peerj.com/articles/19573.pdf
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author Zheng Zhou
Shuyang Chen
Yajun Wang
Shujing Chen
Jinjun Jiang
author_facet Zheng Zhou
Shuyang Chen
Yajun Wang
Shujing Chen
Jinjun Jiang
author_sort Zheng Zhou
collection DOAJ
description Circular RNA (circRNA) is a prominent group of non-coding RNA (ncRNA) that regulates gene expression at the post-transcriptional level in various diseases including acute respiratory distress syndrome (ARDS). In our study, circVAMP3 was a novel circRNA significantly increased in macrophages in an acute lung injury (ALI) model. Through knockdown and overexpression of circVAMP3, we found circVAMP3 promoted the expression of IFN-β in macrophages, which was vital in the pathophysiological process of ARDS. Moreover, we showed that circVAMP3 targeted Stimulator of Interferon Genes (STING) through miR-580-3p by bioinformatics analysis and intervene of miR-580-3p. CircVAMP3 acts as a pro-inflammation ncRNA by regulating the miR-580-3p/STING/IFN-β axis and could be a potential biomarker and therapeutic target for ARDS.
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institution Kabale University
issn 2167-8359
language English
publishDate 2025-06-01
publisher PeerJ Inc.
record_format Article
series PeerJ
spelling doaj-art-1b57fecd579241b1a2bec7fe430d4ff32025-08-20T03:24:22ZengPeerJ Inc.PeerJ2167-83592025-06-0113e1957310.7717/peerj.19573CircVAMP3 promotes acute lung injury progression by regulating the miR-580-3p/STING/IFN-β axis in macrophagesZheng Zhou0Shuyang Chen1Yajun Wang2Shujing Chen3Jinjun Jiang4Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaCircular RNA (circRNA) is a prominent group of non-coding RNA (ncRNA) that regulates gene expression at the post-transcriptional level in various diseases including acute respiratory distress syndrome (ARDS). In our study, circVAMP3 was a novel circRNA significantly increased in macrophages in an acute lung injury (ALI) model. Through knockdown and overexpression of circVAMP3, we found circVAMP3 promoted the expression of IFN-β in macrophages, which was vital in the pathophysiological process of ARDS. Moreover, we showed that circVAMP3 targeted Stimulator of Interferon Genes (STING) through miR-580-3p by bioinformatics analysis and intervene of miR-580-3p. CircVAMP3 acts as a pro-inflammation ncRNA by regulating the miR-580-3p/STING/IFN-β axis and could be a potential biomarker and therapeutic target for ARDS.https://peerj.com/articles/19573.pdfCircVAMP3MacrophagesLipopolysaccharideAcute respiratory distress syndromeInterferon-β
spellingShingle Zheng Zhou
Shuyang Chen
Yajun Wang
Shujing Chen
Jinjun Jiang
CircVAMP3 promotes acute lung injury progression by regulating the miR-580-3p/STING/IFN-β axis in macrophages
PeerJ
CircVAMP3
Macrophages
Lipopolysaccharide
Acute respiratory distress syndrome
Interferon-β
title CircVAMP3 promotes acute lung injury progression by regulating the miR-580-3p/STING/IFN-β axis in macrophages
title_full CircVAMP3 promotes acute lung injury progression by regulating the miR-580-3p/STING/IFN-β axis in macrophages
title_fullStr CircVAMP3 promotes acute lung injury progression by regulating the miR-580-3p/STING/IFN-β axis in macrophages
title_full_unstemmed CircVAMP3 promotes acute lung injury progression by regulating the miR-580-3p/STING/IFN-β axis in macrophages
title_short CircVAMP3 promotes acute lung injury progression by regulating the miR-580-3p/STING/IFN-β axis in macrophages
title_sort circvamp3 promotes acute lung injury progression by regulating the mir 580 3p sting ifn β axis in macrophages
topic CircVAMP3
Macrophages
Lipopolysaccharide
Acute respiratory distress syndrome
Interferon-β
url https://peerj.com/articles/19573.pdf
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AT yajunwang circvamp3promotesacutelunginjuryprogressionbyregulatingthemir5803pstingifnbaxisinmacrophages
AT shujingchen circvamp3promotesacutelunginjuryprogressionbyregulatingthemir5803pstingifnbaxisinmacrophages
AT jinjunjiang circvamp3promotesacutelunginjuryprogressionbyregulatingthemir5803pstingifnbaxisinmacrophages