High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing

Repeated thermal treatment could impair the flavor of instant black tea (IBT) as the volatile compounds and characteristic constituents dramatically vary during the manufacturing process. Utilization of fresh leaves as raw material in IBT manufacture at low temperature may become an alternative to c...

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Main Authors: Junhao Kong, Xiufang Yang, Xiaobo Zuo, Xiaoqin Su, Bing Hu, Xinle Liang
Format: Article
Language:English
Published: Tsinghua University Press 2022-05-01
Series:Food Science and Human Wellness
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Online Access:http://www.sciencedirect.com/science/article/pii/S2213453021001531
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author Junhao Kong
Xiufang Yang
Xiaobo Zuo
Xiaoqin Su
Bing Hu
Xinle Liang
author_facet Junhao Kong
Xiufang Yang
Xiaobo Zuo
Xiaoqin Su
Bing Hu
Xinle Liang
author_sort Junhao Kong
collection DOAJ
description Repeated thermal treatment could impair the flavor of instant black tea (IBT) as the volatile compounds and characteristic constituents dramatically vary during the manufacturing process. Utilization of fresh leaves as raw material in IBT manufacture at low temperature may become an alternative to conventional extraction methods. This study aimed to improve the quality of IBT using fresh tea leaves by a two-stage enzymatic processing. The influence of combined enzymatic oxidation and extraction conditions for maximal extractable flavour (taste and aroma) characteristics from fresh tea leaves were investigated. The changes of aroma profiles and chemical constituents including theaflavins (TFs), thearubigins (TRs), theabrownines (TBs) and TRs/TFs ratio in converted tea brews under two-stage enzyme catalyzed fermentation and extraction conditions were further compared between different treatments, based on which the optimised conditions were determined. During the two-stage enzymatic processing, 75 kinds of volatile compounds were identified in all processed samples, and the contents of TFs, TRs, TBs and TRs/TFs ratio could be optimized by adjusting different procedures and parameters. The overall properties of final product were compared with two types of commercial IBT. High quality IBT with desired properties and antioxidant activity can be manufactured using fresh tea leaves by two-stage enzymatic processing approach.
format Article
id doaj-art-1b533860cbdd49238a46d600727bd463
institution Kabale University
issn 2213-4530
language English
publishDate 2022-05-01
publisher Tsinghua University Press
record_format Article
series Food Science and Human Wellness
spelling doaj-art-1b533860cbdd49238a46d600727bd4632025-02-03T05:38:31ZengTsinghua University PressFood Science and Human Wellness2213-45302022-05-01113676685High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processingJunhao Kong0Xiufang Yang1Xiaobo Zuo2Xiaoqin Su3Bing Hu4Xinle Liang5Department of Biochemical Engineering, Zhejiang Gongshang Univeristy, Hangzhou 310018, China; Hangzhou Tea Research Institute, China COOP, Hangzhou 310016, China; Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resource, Hangzhou 310016, ChinaHangzhou Tea Research Institute, China COOP, Hangzhou 310016, China; Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resource, Hangzhou 310016, China; Haixi Tea Deep Processing Institute, Zhangzhou 363007, ChinaHangzhou Tea Research Institute, China COOP, Hangzhou 310016, China; Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resource, Hangzhou 310016, ChinaHangzhou Tea Research Institute, China COOP, Hangzhou 310016, China; Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resource, Hangzhou 310016, ChinaZhejiang Key Laboratory of Transboundary Applied Technology for Tea Resource, Hangzhou 310016, China; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author at: Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resource, Hangzhou 310016, ChinaDepartment of Biochemical Engineering, Zhejiang Gongshang Univeristy, Hangzhou 310018, China; Corresponding author.Repeated thermal treatment could impair the flavor of instant black tea (IBT) as the volatile compounds and characteristic constituents dramatically vary during the manufacturing process. Utilization of fresh leaves as raw material in IBT manufacture at low temperature may become an alternative to conventional extraction methods. This study aimed to improve the quality of IBT using fresh tea leaves by a two-stage enzymatic processing. The influence of combined enzymatic oxidation and extraction conditions for maximal extractable flavour (taste and aroma) characteristics from fresh tea leaves were investigated. The changes of aroma profiles and chemical constituents including theaflavins (TFs), thearubigins (TRs), theabrownines (TBs) and TRs/TFs ratio in converted tea brews under two-stage enzyme catalyzed fermentation and extraction conditions were further compared between different treatments, based on which the optimised conditions were determined. During the two-stage enzymatic processing, 75 kinds of volatile compounds were identified in all processed samples, and the contents of TFs, TRs, TBs and TRs/TFs ratio could be optimized by adjusting different procedures and parameters. The overall properties of final product were compared with two types of commercial IBT. High quality IBT with desired properties and antioxidant activity can be manufactured using fresh tea leaves by two-stage enzymatic processing approach.http://www.sciencedirect.com/science/article/pii/S2213453021001531Instant black teaLiquid-state fermentationEnzymatic-enhanced extractionQuality characteristics
spellingShingle Junhao Kong
Xiufang Yang
Xiaobo Zuo
Xiaoqin Su
Bing Hu
Xinle Liang
High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing
Food Science and Human Wellness
Instant black tea
Liquid-state fermentation
Enzymatic-enhanced extraction
Quality characteristics
title High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing
title_full High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing
title_fullStr High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing
title_full_unstemmed High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing
title_short High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing
title_sort high quality instant black tea manufactured using fresh tea leaves by two stage submerged enzymatic processing
topic Instant black tea
Liquid-state fermentation
Enzymatic-enhanced extraction
Quality characteristics
url http://www.sciencedirect.com/science/article/pii/S2213453021001531
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