Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR)
In order to evaluate the genetic purity and originality of three common commercial cotton cultivars (Varamin, Khordad and Bakhtegan) using morphological characteristics and microsatellite markers, the seeds of these cultivars along with pure lines in the field in a two-year randomized complete block...
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Seed and Plant Certification and Registration Institute
2023-09-01
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| Series: | علوم و فناوری بذر ایران |
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| Online Access: | https://ijsst.areeo.ac.ir/article_129525_1cd38f00ecd7be807cebc75fa53d38fe.pdf |
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| author | maryam najafian Ghasem tohidloo A. Hamidi farzad paknejad mehdi rezaei |
| author_facet | maryam najafian Ghasem tohidloo A. Hamidi farzad paknejad mehdi rezaei |
| author_sort | maryam najafian |
| collection | DOAJ |
| description | In order to evaluate the genetic purity and originality of three common commercial cotton cultivars (Varamin, Khordad and Bakhtegan) using morphological characteristics and microsatellite markers, the seeds of these cultivars along with pure lines in the field in a two-year randomized complete block design with four replications two methods of field growth test (GOT) and microsatellite markers were tested. The results of the GOT test showed that the pure samples of the cultivars were different from the off-type and non-pure samples in 7 morphological characteristics, and these traits, which are not influenced by the environment, can be used as a key to identify the desired cultivar. In the molecular test, out of 7 markers, 3 pairs were selected due to their resolution and the number of effective alleles (DPL431, DPL0513, and CIR246). Due to the similarity of the results of GOT methods and microsatellite markers and due to the high cost of the molecular method, this method can be used as a quick, case-by-case and complementary method to determine the genetic purity percentage of cultivars. |
| format | Article |
| id | doaj-art-1b460dec23d84448911ef5f0b8e53c8d |
| institution | Kabale University |
| issn | 2322-2646 2588-4638 |
| language | fas |
| publishDate | 2023-09-01 |
| publisher | Seed and Plant Certification and Registration Institute |
| record_format | Article |
| series | علوم و فناوری بذر ایران |
| spelling | doaj-art-1b460dec23d84448911ef5f0b8e53c8d2025-08-20T03:28:10ZfasSeed and Plant Certification and Registration Instituteعلوم و فناوری بذر ایران2322-26462588-46382023-09-01123132410.22092/ijsst.2023.361051.1466129525Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR)maryam najafian0Ghasem tohidloo1A. Hamidi2farzad paknejad3mehdi rezaei4Islamic Azad University, Karaj branchIslamic Azad University, Karaj branchAgriculture Research, Education and Extension Organization-Seed and Plant Certification and Registration InstituteIslamic Azad University, Karaj branchSeed and Plant Certification and Registration Institute (SPCRI), KarajIn order to evaluate the genetic purity and originality of three common commercial cotton cultivars (Varamin, Khordad and Bakhtegan) using morphological characteristics and microsatellite markers, the seeds of these cultivars along with pure lines in the field in a two-year randomized complete block design with four replications two methods of field growth test (GOT) and microsatellite markers were tested. The results of the GOT test showed that the pure samples of the cultivars were different from the off-type and non-pure samples in 7 morphological characteristics, and these traits, which are not influenced by the environment, can be used as a key to identify the desired cultivar. In the molecular test, out of 7 markers, 3 pairs were selected due to their resolution and the number of effective alleles (DPL431, DPL0513, and CIR246). Due to the similarity of the results of GOT methods and microsatellite markers and due to the high cost of the molecular method, this method can be used as a quick, case-by-case and complementary method to determine the genetic purity percentage of cultivars.https://ijsst.areeo.ac.ir/article_129525_1cd38f00ecd7be807cebc75fa53d38fe.pdfkeywords: cotton. genetic purity .testmicrosatellite marker |
| spellingShingle | maryam najafian Ghasem tohidloo A. Hamidi farzad paknejad mehdi rezaei Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR) علوم و فناوری بذر ایران keywords: cotton . genetic purity .test microsatellite marker |
| title | Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR) |
| title_full | Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR) |
| title_fullStr | Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR) |
| title_full_unstemmed | Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR) |
| title_short | Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR) |
| title_sort | assessment of genetic purity of cotton cultivars gossypium hirsutum l using morphological characteristics and microsatellite marker ssr |
| topic | keywords: cotton . genetic purity .test microsatellite marker |
| url | https://ijsst.areeo.ac.ir/article_129525_1cd38f00ecd7be807cebc75fa53d38fe.pdf |
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