Rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging.

A fast and direct combination of techniques for simultaneous mycotoxin and phytoalexin identification in peanut skin and kernel is described. Silica Plate Imprinting Laser Desorption/Ionization Mass Spectrometry Imaging (SPILDI-MSI) is a powerful technique that exhibits great advantages, such as sol...

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Main Authors: Diogo N de Oliveira, Mônica S Ferreira, Rodrigo R Catharino
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090901&type=printable
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author Diogo N de Oliveira
Mônica S Ferreira
Rodrigo R Catharino
author_facet Diogo N de Oliveira
Mônica S Ferreira
Rodrigo R Catharino
author_sort Diogo N de Oliveira
collection DOAJ
description A fast and direct combination of techniques for simultaneous mycotoxin and phytoalexin identification in peanut skin and kernel is described. Silica Plate Imprinting Laser Desorption/Ionization Mass Spectrometry Imaging (SPILDI-MSI) is a powerful technique that exhibits great advantages, such as solvent-free and matrix-free characteristics, as well as no sample preparation or separation steps. It also permits accurate identification of mycotoxins and phytoalexins with unique fingerprint profiles in just a few seconds. Results are expressed as chemical images of the 4 identified types of aflatoxins (B1, B2, G1 and G2) and a stilbenoid (resveratrol). Also, SPILDI-MSI allows the comparison between the spatial distribution of aflatoxins and resveratrol found in kernel and skin. This novel application has proven to be useful for instantaneous qualitative assessment of aflatoxins and stilbenoids both in the peanut skin and kernel and offers precise tracking of fungal contamination in nuts and other foodstuffs.
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publisher Public Library of Science (PLoS)
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spelling doaj-art-1b3f089cb62741148fd3d36e91c90b942025-08-20T02:15:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9090110.1371/journal.pone.0090901Rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging.Diogo N de OliveiraMônica S FerreiraRodrigo R CatharinoA fast and direct combination of techniques for simultaneous mycotoxin and phytoalexin identification in peanut skin and kernel is described. Silica Plate Imprinting Laser Desorption/Ionization Mass Spectrometry Imaging (SPILDI-MSI) is a powerful technique that exhibits great advantages, such as solvent-free and matrix-free characteristics, as well as no sample preparation or separation steps. It also permits accurate identification of mycotoxins and phytoalexins with unique fingerprint profiles in just a few seconds. Results are expressed as chemical images of the 4 identified types of aflatoxins (B1, B2, G1 and G2) and a stilbenoid (resveratrol). Also, SPILDI-MSI allows the comparison between the spatial distribution of aflatoxins and resveratrol found in kernel and skin. This novel application has proven to be useful for instantaneous qualitative assessment of aflatoxins and stilbenoids both in the peanut skin and kernel and offers precise tracking of fungal contamination in nuts and other foodstuffs.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090901&type=printable
spellingShingle Diogo N de Oliveira
Mônica S Ferreira
Rodrigo R Catharino
Rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging.
PLoS ONE
title Rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging.
title_full Rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging.
title_fullStr Rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging.
title_full_unstemmed Rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging.
title_short Rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging.
title_sort rapid and simultaneous in situ assessment of aflatoxins and stilbenes using silica plate imprinting mass spectrometry imaging
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090901&type=printable
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AT monicasferreira rapidandsimultaneousinsituassessmentofaflatoxinsandstilbenesusingsilicaplateimprintingmassspectrometryimaging
AT rodrigorcatharino rapidandsimultaneousinsituassessmentofaflatoxinsandstilbenesusingsilicaplateimprintingmassspectrometryimaging