The Pattern of Copper Release in Copper‐Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture Models

Cancer is a leading cause of death worldwide. Glioblastoma (GBM) is a major challenge in oncology due to its highly invasive nature and limited treatment options. GBM's aggressive migration beyond tumor margins and rapid tumor growth hinders success in patient treatment. Localized therapeutic d...

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Main Authors: Jose I. Garcia‐Peiro, Paula Guerrero‐López, Felipe Hornos, Jose L. Hueso, J. Manuel Garcia‐Aznar, Jesus Santamaria
Format: Article
Language:English
Published: Wiley-VCH 2024-12-01
Series:Small Science
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Online Access:https://doi.org/10.1002/smsc.202400206
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author Jose I. Garcia‐Peiro
Paula Guerrero‐López
Felipe Hornos
Jose L. Hueso
J. Manuel Garcia‐Aznar
Jesus Santamaria
author_facet Jose I. Garcia‐Peiro
Paula Guerrero‐López
Felipe Hornos
Jose L. Hueso
J. Manuel Garcia‐Aznar
Jesus Santamaria
author_sort Jose I. Garcia‐Peiro
collection DOAJ
description Cancer is a leading cause of death worldwide. Glioblastoma (GBM) is a major challenge in oncology due to its highly invasive nature and limited treatment options. GBM's aggressive migration beyond tumor margins and rapid tumor growth hinders success in patient treatment. Localized therapeutic delivery, such as the use of transition metals like copper, is highlighted as a novel therapeutic agent for many potential biomedical applications. Herein, it is aimed to study the effects of Cu release on the proliferation and invasiveness of cancer cells. To this end, novel copper‐based nanostructures with different release patterns are designed. Using a complex 3D cell culture model to mimic the tumor microenvironment, it is shown that different patterns of copper ion release have a strong impact on GBM progression and invasiveness. The findings highlight the importance of optimizing localized copper release patterns to tailor different tumor treatment strategies. They also show the potential and suitability of 3D microchips as instruments to study the behavior of tumor spheroids. In spite of their limitations, these 3D microdevices enable a controlled and close monitoring of the influence of environmental factors (such as the presence of Cu ions) on the proliferation and invasiveness of the cells, with a better approach to reality compared to 2D models and with a more controlled environment, compared to an in vivo model.
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spelling doaj-art-1b3458f8e5d744d8a090f3cb64ccba902025-08-20T02:49:16ZengWiley-VCHSmall Science2688-40462024-12-01412n/an/a10.1002/smsc.202400206The Pattern of Copper Release in Copper‐Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture ModelsJose I. Garcia‐Peiro0Paula Guerrero‐López1Felipe Hornos2Jose L. Hueso3J. Manuel Garcia‐Aznar4Jesus Santamaria5Instituto de Nanociencia y Materiales de Aragon (INMA) CSIC‐Universidad de Zaragoza Campus Rio Ebro Edificio I+D C/ Poeta Mariano Esquillor, s/n 50018 Zaragoza SpainInstituto de Investigación Sanitaria (IIS) de Aragón Avenida San Juan Bosco, 13 50009 Zaragoza SpainInstituto de Nanociencia y Materiales de Aragon (INMA) CSIC‐Universidad de Zaragoza Campus Rio Ebro Edificio I+D C/ Poeta Mariano Esquillor, s/n 50018 Zaragoza SpainInstituto de Nanociencia y Materiales de Aragon (INMA) CSIC‐Universidad de Zaragoza Campus Rio Ebro Edificio I+D C/ Poeta Mariano Esquillor, s/n 50018 Zaragoza SpainInstituto de Investigación Sanitaria (IIS) de Aragón Avenida San Juan Bosco, 13 50009 Zaragoza SpainInstituto de Nanociencia y Materiales de Aragon (INMA) CSIC‐Universidad de Zaragoza Campus Rio Ebro Edificio I+D C/ Poeta Mariano Esquillor, s/n 50018 Zaragoza SpainCancer is a leading cause of death worldwide. Glioblastoma (GBM) is a major challenge in oncology due to its highly invasive nature and limited treatment options. GBM's aggressive migration beyond tumor margins and rapid tumor growth hinders success in patient treatment. Localized therapeutic delivery, such as the use of transition metals like copper, is highlighted as a novel therapeutic agent for many potential biomedical applications. Herein, it is aimed to study the effects of Cu release on the proliferation and invasiveness of cancer cells. To this end, novel copper‐based nanostructures with different release patterns are designed. Using a complex 3D cell culture model to mimic the tumor microenvironment, it is shown that different patterns of copper ion release have a strong impact on GBM progression and invasiveness. The findings highlight the importance of optimizing localized copper release patterns to tailor different tumor treatment strategies. They also show the potential and suitability of 3D microchips as instruments to study the behavior of tumor spheroids. In spite of their limitations, these 3D microdevices enable a controlled and close monitoring of the influence of environmental factors (such as the presence of Cu ions) on the proliferation and invasiveness of the cells, with a better approach to reality compared to 2D models and with a more controlled environment, compared to an in vivo model.https://doi.org/10.1002/smsc.2024002063D culturescoppercore‐shellglioblastomamicrochipsrelease
spellingShingle Jose I. Garcia‐Peiro
Paula Guerrero‐López
Felipe Hornos
Jose L. Hueso
J. Manuel Garcia‐Aznar
Jesus Santamaria
The Pattern of Copper Release in Copper‐Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture Models
Small Science
3D cultures
copper
core‐shell
glioblastoma
microchips
release
title The Pattern of Copper Release in Copper‐Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture Models
title_full The Pattern of Copper Release in Copper‐Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture Models
title_fullStr The Pattern of Copper Release in Copper‐Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture Models
title_full_unstemmed The Pattern of Copper Release in Copper‐Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture Models
title_short The Pattern of Copper Release in Copper‐Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture Models
title_sort pattern of copper release in copper based nanoparticles regulates tumor proliferation and invasiveness in 3d culture models
topic 3D cultures
copper
core‐shell
glioblastoma
microchips
release
url https://doi.org/10.1002/smsc.202400206
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