An ingestible bioimpedance sensing device for wireless monitoring of epithelial barriers
Abstract Existing gastrointestinal (GI) diagnostic tools are unable to non-invasively monitor mucosal tight junction integrity in vivo beyond the esophagus. In the GI tract, local inflammatory processes induce alterations in tight junction proteins, enhancing paracellular ion permeability. Although...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2025-02-01
|
| Series: | Microsystems & Nanoengineering |
| Online Access: | https://doi.org/10.1038/s41378-025-00877-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823861942885810176 |
|---|---|
| author | Brian M. Holt Justin M. Stine Luke A. Beardslee Hammed Ayansola Younggeon Jin Pankaj J. Pasricha Reza Ghodssi |
| author_facet | Brian M. Holt Justin M. Stine Luke A. Beardslee Hammed Ayansola Younggeon Jin Pankaj J. Pasricha Reza Ghodssi |
| author_sort | Brian M. Holt |
| collection | DOAJ |
| description | Abstract Existing gastrointestinal (GI) diagnostic tools are unable to non-invasively monitor mucosal tight junction integrity in vivo beyond the esophagus. In the GI tract, local inflammatory processes induce alterations in tight junction proteins, enhancing paracellular ion permeability. Although transepithelial electrical resistance (TEER) may be used in the laboratory to assess mucosal barrier integrity, there are no existing methodologies for characterizing tight junction dilation in vivo. Addressing this technology gap, intraluminal bioimpedance sensing may be employed as a localized, non-invasive surrogate to TEER electrodes used in cell cultures. Thus far, bioimpedance has only been implemented in esophagogastroduodenoscopy (EGD) due to the need for external electronics connections. In this work, we develop a novel, noise-resilient Bluetooth-enabled ingestible device for the continuous, non-invasive measurement of intestinal mucosal “leakiness.” As a proof-of-concept, we validate wireless impedance readout on excised porcine tissues in motion. Through an animal study, we demonstrate how the device exhibits altered impedance response to tight junction dilation induced on mice colonic tissue through calcium-chelator exposure. Device measurements are validated using standard benchtop methods for assessing mucosal permeability. |
| format | Article |
| id | doaj-art-1b34051e41784a8cbfeeb4e08b721dd2 |
| institution | Kabale University |
| issn | 2055-7434 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Microsystems & Nanoengineering |
| spelling | doaj-art-1b34051e41784a8cbfeeb4e08b721dd22025-02-09T12:41:48ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342025-02-0111111410.1038/s41378-025-00877-8An ingestible bioimpedance sensing device for wireless monitoring of epithelial barriersBrian M. Holt0Justin M. Stine1Luke A. Beardslee2Hammed Ayansola3Younggeon Jin4Pankaj J. Pasricha5Reza Ghodssi6Department of Electrical and Computer Engineering, University of MarylandMatrix Lab at A. James Clark School of Engineering, University of MarylandInstitute for Systems Research, University of MarylandDepartment of Animal & Avian Sciences, University of MarylandDepartment of Animal & Avian Sciences, University of MarylandMayo Clinic HospitalDepartment of Electrical and Computer Engineering, University of MarylandAbstract Existing gastrointestinal (GI) diagnostic tools are unable to non-invasively monitor mucosal tight junction integrity in vivo beyond the esophagus. In the GI tract, local inflammatory processes induce alterations in tight junction proteins, enhancing paracellular ion permeability. Although transepithelial electrical resistance (TEER) may be used in the laboratory to assess mucosal barrier integrity, there are no existing methodologies for characterizing tight junction dilation in vivo. Addressing this technology gap, intraluminal bioimpedance sensing may be employed as a localized, non-invasive surrogate to TEER electrodes used in cell cultures. Thus far, bioimpedance has only been implemented in esophagogastroduodenoscopy (EGD) due to the need for external electronics connections. In this work, we develop a novel, noise-resilient Bluetooth-enabled ingestible device for the continuous, non-invasive measurement of intestinal mucosal “leakiness.” As a proof-of-concept, we validate wireless impedance readout on excised porcine tissues in motion. Through an animal study, we demonstrate how the device exhibits altered impedance response to tight junction dilation induced on mice colonic tissue through calcium-chelator exposure. Device measurements are validated using standard benchtop methods for assessing mucosal permeability.https://doi.org/10.1038/s41378-025-00877-8 |
| spellingShingle | Brian M. Holt Justin M. Stine Luke A. Beardslee Hammed Ayansola Younggeon Jin Pankaj J. Pasricha Reza Ghodssi An ingestible bioimpedance sensing device for wireless monitoring of epithelial barriers Microsystems & Nanoengineering |
| title | An ingestible bioimpedance sensing device for wireless monitoring of epithelial barriers |
| title_full | An ingestible bioimpedance sensing device for wireless monitoring of epithelial barriers |
| title_fullStr | An ingestible bioimpedance sensing device for wireless monitoring of epithelial barriers |
| title_full_unstemmed | An ingestible bioimpedance sensing device for wireless monitoring of epithelial barriers |
| title_short | An ingestible bioimpedance sensing device for wireless monitoring of epithelial barriers |
| title_sort | ingestible bioimpedance sensing device for wireless monitoring of epithelial barriers |
| url | https://doi.org/10.1038/s41378-025-00877-8 |
| work_keys_str_mv | AT brianmholt aningestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT justinmstine aningestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT lukeabeardslee aningestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT hammedayansola aningestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT younggeonjin aningestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT pankajjpasricha aningestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT rezaghodssi aningestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT brianmholt ingestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT justinmstine ingestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT lukeabeardslee ingestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT hammedayansola ingestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT younggeonjin ingestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT pankajjpasricha ingestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers AT rezaghodssi ingestiblebioimpedancesensingdeviceforwirelessmonitoringofepithelialbarriers |