Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP)

Paratuberculosis progresses more quickly in young red deer than in sheep or cattle. This study describes the clinical, immunological and pathological changes over a 50-week period in fourteen 4-month-old red deer that received heavy oral challenge with Mycobacterium avium subsp. paratuberculosis (MA...

Full description

Saved in:
Bibliographic Details
Main Authors: Colin Mackintosh, Gary Clark, Brendan Tolentino, Simon Liggett, Geoff de Lisle, Frank Griffin
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Veterinary Medicine International
Online Access:http://dx.doi.org/10.1155/2012/931948
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850235132069281792
author Colin Mackintosh
Gary Clark
Brendan Tolentino
Simon Liggett
Geoff de Lisle
Frank Griffin
author_facet Colin Mackintosh
Gary Clark
Brendan Tolentino
Simon Liggett
Geoff de Lisle
Frank Griffin
author_sort Colin Mackintosh
collection DOAJ
description Paratuberculosis progresses more quickly in young red deer than in sheep or cattle. This study describes the clinical, immunological and pathological changes over a 50-week period in fourteen 4-month-old red deer that received heavy oral challenge with Mycobacterium avium subsp. paratuberculosis (MAP). At 4 and 12 weeks post challenge they were anaesthetized and a section of jejunal lymph node was surgically removed for culture, histopathology, and genetic studies. All 14 deer became infected, none were clinically affected, and they had varying degrees of subclinical disease when killed at week 50. Week 4 biopsies showed no paratuberculosis lesions, but MAP was cultured from all animals. At weeks 12 and 50 histopathological lesions ranged from mild to severe with corresponding low-to-high antibody titres, which peaked at 12–24 weeks. IFN-γ responses peaked at 8–15 weeks and were higher in mildly affected animals than in those with severe lesions.
format Article
id doaj-art-1b2ca9b87d5e4d59aba2fccbd7c879ac
institution OA Journals
issn 2090-8113
2042-0048
language English
publishDate 2012-01-01
publisher Wiley
record_format Article
series Veterinary Medicine International
spelling doaj-art-1b2ca9b87d5e4d59aba2fccbd7c879ac2025-08-20T02:02:21ZengWileyVeterinary Medicine International2090-81132042-00482012-01-01201210.1155/2012/931948931948Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP)Colin Mackintosh0Gary Clark1Brendan Tolentino2Simon Liggett3Geoff de Lisle4Frank Griffin5AgResearch Invermay, P.O. Box 50034, Mosgiel, New Zealand14 Rimu Lane, Wanaka 9305, New ZealandAgResearch Invermay, P.O. Box 50034, Mosgiel, New ZealandDisease Research Laboratory, University of Otago, P.O. Box 56, Dunedin, New ZealandAgResearch Wallaceville, P.O. Box 40063, Upper Hutt, New ZealandDisease Research Laboratory, University of Otago, P.O. Box 56, Dunedin, New ZealandParatuberculosis progresses more quickly in young red deer than in sheep or cattle. This study describes the clinical, immunological and pathological changes over a 50-week period in fourteen 4-month-old red deer that received heavy oral challenge with Mycobacterium avium subsp. paratuberculosis (MAP). At 4 and 12 weeks post challenge they were anaesthetized and a section of jejunal lymph node was surgically removed for culture, histopathology, and genetic studies. All 14 deer became infected, none were clinically affected, and they had varying degrees of subclinical disease when killed at week 50. Week 4 biopsies showed no paratuberculosis lesions, but MAP was cultured from all animals. At weeks 12 and 50 histopathological lesions ranged from mild to severe with corresponding low-to-high antibody titres, which peaked at 12–24 weeks. IFN-γ responses peaked at 8–15 weeks and were higher in mildly affected animals than in those with severe lesions.http://dx.doi.org/10.1155/2012/931948
spellingShingle Colin Mackintosh
Gary Clark
Brendan Tolentino
Simon Liggett
Geoff de Lisle
Frank Griffin
Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP)
Veterinary Medicine International
title Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP)
title_full Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP)
title_fullStr Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP)
title_full_unstemmed Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP)
title_short Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP)
title_sort longitudinal pathogenesis study of young red deer cervus elaphus after experimental challenge with mycobacterium avium subsp paratuberculosis map
url http://dx.doi.org/10.1155/2012/931948
work_keys_str_mv AT colinmackintosh longitudinalpathogenesisstudyofyoungreddeercervuselaphusafterexperimentalchallengewithmycobacteriumaviumsubspparatuberculosismap
AT garyclark longitudinalpathogenesisstudyofyoungreddeercervuselaphusafterexperimentalchallengewithmycobacteriumaviumsubspparatuberculosismap
AT brendantolentino longitudinalpathogenesisstudyofyoungreddeercervuselaphusafterexperimentalchallengewithmycobacteriumaviumsubspparatuberculosismap
AT simonliggett longitudinalpathogenesisstudyofyoungreddeercervuselaphusafterexperimentalchallengewithmycobacteriumaviumsubspparatuberculosismap
AT geoffdelisle longitudinalpathogenesisstudyofyoungreddeercervuselaphusafterexperimentalchallengewithmycobacteriumaviumsubspparatuberculosismap
AT frankgriffin longitudinalpathogenesisstudyofyoungreddeercervuselaphusafterexperimentalchallengewithmycobacteriumaviumsubspparatuberculosismap