Synthesis and Characterization of Chitosan Particles Loaded with Antioxidants Extracted from Chia (Salvia hispanica L.) Seeds
Chia (Salvia hispanica L.) seeds contain antioxidants with great benefits for health and are widely used in the food industry. Antioxidants can be degraded by environmental factors, decreasing their biological activity. Their encapsulation in chitosan (CH) particles represents an alternative to prot...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/5540543 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849308606661394432 |
|---|---|
| author | Gema Morales-Olán Silvia Luna-Suárez Juan De Dios Figueroa-Cárdenas Monica Corea Marlon Rojas-López |
| author_facet | Gema Morales-Olán Silvia Luna-Suárez Juan De Dios Figueroa-Cárdenas Monica Corea Marlon Rojas-López |
| author_sort | Gema Morales-Olán |
| collection | DOAJ |
| description | Chia (Salvia hispanica L.) seeds contain antioxidants with great benefits for health and are widely used in the food industry. Antioxidants can be degraded by environmental factors, decreasing their biological activity. Their encapsulation in chitosan (CH) particles represents an alternative to protect them and increases their application. The encapsulation efficiency (%EE) of the antioxidants in the CH particles depends on the synthesis conditions. In this study, two methods for encapsulation of chia extract in chitosan particles were evaluated: method A, 0.05% CH in 1% acetic acid was mixed with 0.07% of tripolyphosphate (TPP) and method B, 0.3% CH in 2% acetic acid was mixed with 1% TPP. The results showed that the %EE decreased with the concentration of the extract, and the FTIR analysis suggested that the compounds of the extract were adsorbed on the surface of the particles. Dynamic light scattering and zeta potential analysis showed that the particles of method A are unstable and with a tendency to agglomerate, and the particles of method B are stable. The highest %EE was obtained with 0.2 mg·mL−1 (method A) and 1.0 mg·mL−1 (method B) of the extract. The higher loading capacity (%LC) (16–72%) was exhibited by the particles of method A. The best particle yield (62–69%) was observed for method B. The particles with the extract adsorbed showed antioxidant activity (5–60%) at 25°C; however, in the particles with the extract encapsulated, the activity increased after subjecting to acidic conditions at 40°C due to the breakdown of the particles. The results obtained will allow choosing the appropriate conditions for the synthesis of chitosan particles loaded with chia extracts with specific characteristics (%EE, %LC, size, and type) according to their future applications. The particles could be used in food and pharmaceutical industries and even in edible films for food packaging. |
| format | Article |
| id | doaj-art-1b2592c14ebf4aa09b81f8d0b5cbe6ba |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-1b2592c14ebf4aa09b81f8d0b5cbe6ba2025-08-20T03:54:24ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792021-01-01202110.1155/2021/55405435540543Synthesis and Characterization of Chitosan Particles Loaded with Antioxidants Extracted from Chia (Salvia hispanica L.) SeedsGema Morales-Olán0Silvia Luna-Suárez1Juan De Dios Figueroa-Cárdenas2Monica Corea3Marlon Rojas-López4Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Ex Hacienda De San Juan Molino, Carretera Estatal Santa Ines, Tecuexcomac-Tepetitla. Km. 1.5, Tepetitla, Tlaxcala 90700, MexicoInstituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Ex Hacienda De San Juan Molino, Carretera Estatal Santa Ines, Tecuexcomac-Tepetitla. Km. 1.5, Tepetitla, Tlaxcala 90700, MexicoCINVESTAV Unidad Querétaro, Libramiento Norponiente No. 2000, Fracc Real de Juriquilla, Querétaro, Qro 76230, MexicoInstituto Politécnico Nacional, Escuela Superior de Ingenieria Química e Industrias Extractivas, UPALM, Zacatenco, Ciudad de México 07732, MexicoInstituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Ex Hacienda De San Juan Molino, Carretera Estatal Santa Ines, Tecuexcomac-Tepetitla. Km. 1.5, Tepetitla, Tlaxcala 90700, MexicoChia (Salvia hispanica L.) seeds contain antioxidants with great benefits for health and are widely used in the food industry. Antioxidants can be degraded by environmental factors, decreasing their biological activity. Their encapsulation in chitosan (CH) particles represents an alternative to protect them and increases their application. The encapsulation efficiency (%EE) of the antioxidants in the CH particles depends on the synthesis conditions. In this study, two methods for encapsulation of chia extract in chitosan particles were evaluated: method A, 0.05% CH in 1% acetic acid was mixed with 0.07% of tripolyphosphate (TPP) and method B, 0.3% CH in 2% acetic acid was mixed with 1% TPP. The results showed that the %EE decreased with the concentration of the extract, and the FTIR analysis suggested that the compounds of the extract were adsorbed on the surface of the particles. Dynamic light scattering and zeta potential analysis showed that the particles of method A are unstable and with a tendency to agglomerate, and the particles of method B are stable. The highest %EE was obtained with 0.2 mg·mL−1 (method A) and 1.0 mg·mL−1 (method B) of the extract. The higher loading capacity (%LC) (16–72%) was exhibited by the particles of method A. The best particle yield (62–69%) was observed for method B. The particles with the extract adsorbed showed antioxidant activity (5–60%) at 25°C; however, in the particles with the extract encapsulated, the activity increased after subjecting to acidic conditions at 40°C due to the breakdown of the particles. The results obtained will allow choosing the appropriate conditions for the synthesis of chitosan particles loaded with chia extracts with specific characteristics (%EE, %LC, size, and type) according to their future applications. The particles could be used in food and pharmaceutical industries and even in edible films for food packaging.http://dx.doi.org/10.1155/2021/5540543 |
| spellingShingle | Gema Morales-Olán Silvia Luna-Suárez Juan De Dios Figueroa-Cárdenas Monica Corea Marlon Rojas-López Synthesis and Characterization of Chitosan Particles Loaded with Antioxidants Extracted from Chia (Salvia hispanica L.) Seeds International Journal of Analytical Chemistry |
| title | Synthesis and Characterization of Chitosan Particles Loaded with Antioxidants Extracted from Chia (Salvia hispanica L.) Seeds |
| title_full | Synthesis and Characterization of Chitosan Particles Loaded with Antioxidants Extracted from Chia (Salvia hispanica L.) Seeds |
| title_fullStr | Synthesis and Characterization of Chitosan Particles Loaded with Antioxidants Extracted from Chia (Salvia hispanica L.) Seeds |
| title_full_unstemmed | Synthesis and Characterization of Chitosan Particles Loaded with Antioxidants Extracted from Chia (Salvia hispanica L.) Seeds |
| title_short | Synthesis and Characterization of Chitosan Particles Loaded with Antioxidants Extracted from Chia (Salvia hispanica L.) Seeds |
| title_sort | synthesis and characterization of chitosan particles loaded with antioxidants extracted from chia salvia hispanica l seeds |
| url | http://dx.doi.org/10.1155/2021/5540543 |
| work_keys_str_mv | AT gemamoralesolan synthesisandcharacterizationofchitosanparticlesloadedwithantioxidantsextractedfromchiasalviahispanicalseeds AT silvialunasuarez synthesisandcharacterizationofchitosanparticlesloadedwithantioxidantsextractedfromchiasalviahispanicalseeds AT juandediosfigueroacardenas synthesisandcharacterizationofchitosanparticlesloadedwithantioxidantsextractedfromchiasalviahispanicalseeds AT monicacorea synthesisandcharacterizationofchitosanparticlesloadedwithantioxidantsextractedfromchiasalviahispanicalseeds AT marlonrojaslopez synthesisandcharacterizationofchitosanparticlesloadedwithantioxidantsextractedfromchiasalviahispanicalseeds |