Enrichment of Second Generation Ethanol Concentration Obtained from Napier Grass Pretreated with Sulfuric Acid and Hydrothermal Pretreatment

Enhancing sugar concentration is crucial for improving ethanol yield in biorefinery processes, enabling more efficient downstream recovery. This study investigates the hydrothermal pretreatment of Napier grass with 2% sulfuric acid to boost sugar recovery and ethanol production by incorporating a co...

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Main Authors: Phakeenuya Vanarat, Panakkal Elizabeth Jayex, Gundupalli Marttin Paulraj, Klinjapo Roungdao, Sriariyanun Malinee, Tantayotai Prapakorn
Format: Article
Language:English
Published: EDP Sciences 2025-01-01
Series:E3S Web of Conferences
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Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/02/e3sconf_icome2025_02007.pdf
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author Phakeenuya Vanarat
Panakkal Elizabeth Jayex
Gundupalli Marttin Paulraj
Klinjapo Roungdao
Sriariyanun Malinee
Tantayotai Prapakorn
author_facet Phakeenuya Vanarat
Panakkal Elizabeth Jayex
Gundupalli Marttin Paulraj
Klinjapo Roungdao
Sriariyanun Malinee
Tantayotai Prapakorn
author_sort Phakeenuya Vanarat
collection DOAJ
description Enhancing sugar concentration is crucial for improving ethanol yield in biorefinery processes, enabling more efficient downstream recovery. This study investigates the hydrothermal pretreatment of Napier grass with 2% sulfuric acid to boost sugar recovery and ethanol production by incorporating a concentration step. After pretreatment, the liquid fractions were concentrated two-fold and four-fold through rotary evaporation and freeze-drying, resulting in a significant increase in sugar levels, with a 3.5-fold rise in sugar concentration achieved through rotary evaporation compared to unconcentrated samples. However, ethanol production was limited by elevated levels of inhibitors, such as acetic acid and furfural. The maximum ethanol concentration reached was 2.43%, from a liquid fraction concentrated four-fold. These results highlight the necessity of concentration techniques to improve sugar recovery, while also emphasizing the importance of removing inhibitors to increase ethanol yields and enhance the overall efficiency of biorefining processes.
format Article
id doaj-art-1b1d2b86fdaf4ab6a1f499454a587108
institution Kabale University
issn 2267-1242
language English
publishDate 2025-01-01
publisher EDP Sciences
record_format Article
series E3S Web of Conferences
spelling doaj-art-1b1d2b86fdaf4ab6a1f499454a5871082025-01-16T11:22:35ZengEDP SciencesE3S Web of Conferences2267-12422025-01-016020200710.1051/e3sconf/202560202007e3sconf_icome2025_02007Enrichment of Second Generation Ethanol Concentration Obtained from Napier Grass Pretreated with Sulfuric Acid and Hydrothermal PretreatmentPhakeenuya Vanarat0Panakkal Elizabeth Jayex1Gundupalli Marttin Paulraj2Klinjapo Roungdao3Sriariyanun Malinee4Tantayotai Prapakorn5Department of Biotechnology, Faculty of Applied Science, King Mongkut’s University of Technology North BangkokBiorefinery and Process Automation Engineering Center, Department of Chemical and Process Engineering, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North BangkokFaculty of Agricultural, Life and Environmental Sci Ag, Food & Nutri Sci Dept, University of AlbertaTheophane Venard School Of Biotechnology, Assumption University ofBiorefinery and Process Automation Engineering Center, Department of Chemical and Process Engineering, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North BangkokDepartment of Microbiology, Faculty of Science, Srinakharinwirot UniversityEnhancing sugar concentration is crucial for improving ethanol yield in biorefinery processes, enabling more efficient downstream recovery. This study investigates the hydrothermal pretreatment of Napier grass with 2% sulfuric acid to boost sugar recovery and ethanol production by incorporating a concentration step. After pretreatment, the liquid fractions were concentrated two-fold and four-fold through rotary evaporation and freeze-drying, resulting in a significant increase in sugar levels, with a 3.5-fold rise in sugar concentration achieved through rotary evaporation compared to unconcentrated samples. However, ethanol production was limited by elevated levels of inhibitors, such as acetic acid and furfural. The maximum ethanol concentration reached was 2.43%, from a liquid fraction concentrated four-fold. These results highlight the necessity of concentration techniques to improve sugar recovery, while also emphasizing the importance of removing inhibitors to increase ethanol yields and enhance the overall efficiency of biorefining processes.https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/02/e3sconf_icome2025_02007.pdfbiofuelbiorefinerycircular economylignocellulose biomasspretreatment
spellingShingle Phakeenuya Vanarat
Panakkal Elizabeth Jayex
Gundupalli Marttin Paulraj
Klinjapo Roungdao
Sriariyanun Malinee
Tantayotai Prapakorn
Enrichment of Second Generation Ethanol Concentration Obtained from Napier Grass Pretreated with Sulfuric Acid and Hydrothermal Pretreatment
E3S Web of Conferences
biofuel
biorefinery
circular economy
lignocellulose biomass
pretreatment
title Enrichment of Second Generation Ethanol Concentration Obtained from Napier Grass Pretreated with Sulfuric Acid and Hydrothermal Pretreatment
title_full Enrichment of Second Generation Ethanol Concentration Obtained from Napier Grass Pretreated with Sulfuric Acid and Hydrothermal Pretreatment
title_fullStr Enrichment of Second Generation Ethanol Concentration Obtained from Napier Grass Pretreated with Sulfuric Acid and Hydrothermal Pretreatment
title_full_unstemmed Enrichment of Second Generation Ethanol Concentration Obtained from Napier Grass Pretreated with Sulfuric Acid and Hydrothermal Pretreatment
title_short Enrichment of Second Generation Ethanol Concentration Obtained from Napier Grass Pretreated with Sulfuric Acid and Hydrothermal Pretreatment
title_sort enrichment of second generation ethanol concentration obtained from napier grass pretreated with sulfuric acid and hydrothermal pretreatment
topic biofuel
biorefinery
circular economy
lignocellulose biomass
pretreatment
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/02/e3sconf_icome2025_02007.pdf
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AT panakkalelizabethjayex enrichmentofsecondgenerationethanolconcentrationobtainedfromnapiergrasspretreatedwithsulfuricacidandhydrothermalpretreatment
AT gundupallimarttinpaulraj enrichmentofsecondgenerationethanolconcentrationobtainedfromnapiergrasspretreatedwithsulfuricacidandhydrothermalpretreatment
AT klinjaporoungdao enrichmentofsecondgenerationethanolconcentrationobtainedfromnapiergrasspretreatedwithsulfuricacidandhydrothermalpretreatment
AT sriariyanunmalinee enrichmentofsecondgenerationethanolconcentrationobtainedfromnapiergrasspretreatedwithsulfuricacidandhydrothermalpretreatment
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