Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive Roots
Verticillium wilt, caused by <i>Verticillium dahliae</i>, poses a significant threat to olive trees (<i>Olea europaea</i> L.). The isolation of endophytic <i>Streptomyces</i> strains from olive roots has led to the discovery of several strains showing strong antif...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/7/1622 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850071152942120960 |
|---|---|
| author | Carla Calvo-Peña Marina Ruiz-Muñoz Imen Nouioui Sarah Kirstein Meina Neumann-Schaal José María Sánchez-López Seyedehtannaz Ghoreshizadeh Rebeca Cobos Juan José R. Coque |
| author_facet | Carla Calvo-Peña Marina Ruiz-Muñoz Imen Nouioui Sarah Kirstein Meina Neumann-Schaal José María Sánchez-López Seyedehtannaz Ghoreshizadeh Rebeca Cobos Juan José R. Coque |
| author_sort | Carla Calvo-Peña |
| collection | DOAJ |
| description | Verticillium wilt, caused by <i>Verticillium dahliae</i>, poses a significant threat to olive trees (<i>Olea europaea</i> L.). The isolation of endophytic <i>Streptomyces</i> strains from olive roots has led to the discovery of several strains showing strong antifungal activity against <i>V. dahliae</i>, as demonstrated through in vitro and small-scale soil experiments. Molecular analyses confirmed that strain OE54 belongs to <i>Streptomyces iranensis</i>. The main antifungal compound identified in this strain was rapamycin. Rapamycin displayed potent antifungal effects, notably inhibiting conidiospore germination (IC<sub>50</sub> = 87.36 μg/mL) and the hyphal growth of <i>V. dahliae</i>, with a minimum inhibitory concentration (MIC<sub>50</sub>) of 3.91 ng/mL. Additionally, a second rapamycin-producing strain, OE57<sup>T</sup>, was isolated. Phenotypic and genotypic analyses indicated that OE57<sup>T</sup> represents a new species, which is proposed to be named <i>Streptomyces lacaronensis</i> sp. nov., with OE57<sup>T</sup> designated as the type strain (=DSM 118741<sup>T</sup>; CECT 31164<sup>T</sup>). The discovery of two endophytic rapamycin-producing <i>Streptomyces</i> strains residing within olive roots is especially notable, given the rarity of rapamycin production among microorganisms. These findings highlight the potential of rapamycin-producing <i>Streptomyces</i> strains in developing biofertilizers to manage <i>V. dahliae</i> and reduce the impact of Verticillium wilt on olive trees and other crops. |
| format | Article |
| id | doaj-art-1b1554ca9ba54981bf0d8e8648e55539 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-1b1554ca9ba54981bf0d8e8648e555392025-08-20T02:47:22ZengMDPI AGMicroorganisms2076-26072025-07-01137162210.3390/microorganisms13071622Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive RootsCarla Calvo-Peña0Marina Ruiz-Muñoz1Imen Nouioui2Sarah Kirstein3Meina Neumann-Schaal4José María Sánchez-López5Seyedehtannaz Ghoreshizadeh6Rebeca Cobos7Juan José R. Coque8Instituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainInstituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainGerman Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, GermanyGerman Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, GermanyGerman Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, GermanyBiomar Microbial Technologies, 24009 Armunia, SpainInstituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainInstituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainInstituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainVerticillium wilt, caused by <i>Verticillium dahliae</i>, poses a significant threat to olive trees (<i>Olea europaea</i> L.). The isolation of endophytic <i>Streptomyces</i> strains from olive roots has led to the discovery of several strains showing strong antifungal activity against <i>V. dahliae</i>, as demonstrated through in vitro and small-scale soil experiments. Molecular analyses confirmed that strain OE54 belongs to <i>Streptomyces iranensis</i>. The main antifungal compound identified in this strain was rapamycin. Rapamycin displayed potent antifungal effects, notably inhibiting conidiospore germination (IC<sub>50</sub> = 87.36 μg/mL) and the hyphal growth of <i>V. dahliae</i>, with a minimum inhibitory concentration (MIC<sub>50</sub>) of 3.91 ng/mL. Additionally, a second rapamycin-producing strain, OE57<sup>T</sup>, was isolated. Phenotypic and genotypic analyses indicated that OE57<sup>T</sup> represents a new species, which is proposed to be named <i>Streptomyces lacaronensis</i> sp. nov., with OE57<sup>T</sup> designated as the type strain (=DSM 118741<sup>T</sup>; CECT 31164<sup>T</sup>). The discovery of two endophytic rapamycin-producing <i>Streptomyces</i> strains residing within olive roots is especially notable, given the rarity of rapamycin production among microorganisms. These findings highlight the potential of rapamycin-producing <i>Streptomyces</i> strains in developing biofertilizers to manage <i>V. dahliae</i> and reduce the impact of Verticillium wilt on olive trees and other crops.https://www.mdpi.com/2076-2607/13/7/1622Verticillium wiltoliveendophytic streptomycetesbiocontrolantifungal compoundsrapamycin |
| spellingShingle | Carla Calvo-Peña Marina Ruiz-Muñoz Imen Nouioui Sarah Kirstein Meina Neumann-Schaal José María Sánchez-López Seyedehtannaz Ghoreshizadeh Rebeca Cobos Juan José R. Coque Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive Roots Microorganisms Verticillium wilt olive endophytic streptomycetes biocontrol antifungal compounds rapamycin |
| title | Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive Roots |
| title_full | Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive Roots |
| title_fullStr | Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive Roots |
| title_full_unstemmed | Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive Roots |
| title_short | Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive Roots |
| title_sort | rapamycin plays a pivotal role in the potent antifungal activity exhibited against i verticillium dahliae i by i streptomyces iranensis i oe54 and i streptomyces lacaronensis i sp nov isolated from olive roots |
| topic | Verticillium wilt olive endophytic streptomycetes biocontrol antifungal compounds rapamycin |
| url | https://www.mdpi.com/2076-2607/13/7/1622 |
| work_keys_str_mv | AT carlacalvopena rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots AT marinaruizmunoz rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots AT imennouioui rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots AT sarahkirstein rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots AT meinaneumannschaal rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots AT josemariasanchezlopez rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots AT seyedehtannazghoreshizadeh rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots AT rebecacobos rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots AT juanjosercoque rapamycinplaysapivotalroleinthepotentantifungalactivityexhibitedagainstiverticilliumdahliaeibyistreptomycesiranensisioe54andistreptomyceslacaronensisispnovisolatedfromoliveroots |