DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS
Background: Diabetes Mellitus (DM) patients are susceptible to wound infections brought on by S. aureus bacteria, in dealing with infections caused by bacteria, antibiotic treatment was conducted. Nevertheless, misapplication of antibiotics might lead to drug resistance or MRSA. Panton-Valentine Leu...
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Universitas Airlangga
2023-07-01
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| Series: | Journal of Vocational Health Studies |
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| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/38747 |
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| author | Prisma Anjarlena Suliati Wisnu Istanto Retno Sasongkowati |
| author_facet | Prisma Anjarlena Suliati Wisnu Istanto Retno Sasongkowati |
| author_sort | Prisma Anjarlena |
| collection | DOAJ |
| description | Background: Diabetes Mellitus (DM) patients are susceptible to wound infections brought on by S. aureus bacteria, in dealing with infections caused by bacteria, antibiotic treatment was conducted. Nevertheless, misapplication of antibiotics might lead to drug resistance or MRSA. Panton-Valentine Leukocidin (PVL) is a crucial factor of S. aureus pathogenicity. Skin and soft tissue infections can be caused by the PVL gene. Purpose: To detect PVL gene in ulcer swabs of diabetic mellitus patients against MRSA strains. Method: Employing descriptive quantitative data analysis of PVL gene detection observations on ulcer swab of diabetes mellitus patients using the RT-PCR method. Ulcer swab samples were collected at Dharmahusada and Banyu Urip Diabetes Wound Specialist Hospital, Surabaya, East Java, Indonesia. The research was conducted at the Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Bacteriology Laboratory for the isolation and identification of MRSA and Molecular Biology Laboratory for the detection of the PVL gene in April–May 2022. Result: From 30 samples of ulcer swabs, 8 samples were positive for MRSA and 22 samples were negative for MRSA. After that, the MRSA positive samples were detected using the RT-PCR method to detect the PVL gene. The results of PVL detection were obtained from 6 samples (75%) positive for detecting the PVL gene and 2 samples (25%) negative for detecting the PVL gene. Conclusion: The results of the study showed that ulcer swabs from diabetes mellitus patients had the PVL gene against MRSA. |
| format | Article |
| id | doaj-art-1b12a2eccf32417eb4e7ba14b2d6f3ec |
| institution | Kabale University |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-1b12a2eccf32417eb4e7ba14b2d6f3ec2025-02-03T04:58:56ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2023-07-0171172310.20473/jvhs.V7.I1.2023.17-2336817DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTSPrisma Anjarlena0https://orcid.org/0009-0007-0260-136XSuliati1https://orcid.org/0009-0003-4921-0308Wisnu Istanto2https://orcid.org/0000-0001-8392-0272Retno Sasongkowati3https://orcid.org/0009-0000-3213-3199Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, IndonesiaDepartment of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, IndonesiaDepartment of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, IndonesiaDepartment of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, IndonesiaBackground: Diabetes Mellitus (DM) patients are susceptible to wound infections brought on by S. aureus bacteria, in dealing with infections caused by bacteria, antibiotic treatment was conducted. Nevertheless, misapplication of antibiotics might lead to drug resistance or MRSA. Panton-Valentine Leukocidin (PVL) is a crucial factor of S. aureus pathogenicity. Skin and soft tissue infections can be caused by the PVL gene. Purpose: To detect PVL gene in ulcer swabs of diabetic mellitus patients against MRSA strains. Method: Employing descriptive quantitative data analysis of PVL gene detection observations on ulcer swab of diabetes mellitus patients using the RT-PCR method. Ulcer swab samples were collected at Dharmahusada and Banyu Urip Diabetes Wound Specialist Hospital, Surabaya, East Java, Indonesia. The research was conducted at the Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Bacteriology Laboratory for the isolation and identification of MRSA and Molecular Biology Laboratory for the detection of the PVL gene in April–May 2022. Result: From 30 samples of ulcer swabs, 8 samples were positive for MRSA and 22 samples were negative for MRSA. After that, the MRSA positive samples were detected using the RT-PCR method to detect the PVL gene. The results of PVL detection were obtained from 6 samples (75%) positive for detecting the PVL gene and 2 samples (25%) negative for detecting the PVL gene. Conclusion: The results of the study showed that ulcer swabs from diabetes mellitus patients had the PVL gene against MRSA.https://e-journal.unair.ac.id/JVHS/article/view/38747diabetes mellitus ulcer swabmrsapvl gene |
| spellingShingle | Prisma Anjarlena Suliati Wisnu Istanto Retno Sasongkowati DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS Journal of Vocational Health Studies diabetes mellitus ulcer swab mrsa pvl gene |
| title | DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS |
| title_full | DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS |
| title_fullStr | DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS |
| title_full_unstemmed | DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS |
| title_short | DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS |
| title_sort | detection of panton valentine leukocidin pvl gene against methicillin resistant staphylococcus aureus mrsa in diabetic ulcer patients |
| topic | diabetes mellitus ulcer swab mrsa pvl gene |
| url | https://e-journal.unair.ac.id/JVHS/article/view/38747 |
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