Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistance
Soybean (Glycine max) is one of the most important industrial and oilseed crops; however, the yield is threatened by the invasion of various pathogens. Soybean stem and root rot, caused by Phytophthora sojae, is a destructive disease that significantly damages soybean production worldwide. C2H2 zinc...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Plant Signaling & Behavior |
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| Online Access: | http://dx.doi.org/10.1080/15592324.2025.2481185 |
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| author | Yuting Chen Xinyue Liu Yanyan Zhou Yu Zheng Yating Xiao Xingxing Yuan Qiang Yan Xin Chen |
| author_facet | Yuting Chen Xinyue Liu Yanyan Zhou Yu Zheng Yating Xiao Xingxing Yuan Qiang Yan Xin Chen |
| author_sort | Yuting Chen |
| collection | DOAJ |
| description | Soybean (Glycine max) is one of the most important industrial and oilseed crops; however, the yield is threatened by the invasion of various pathogens. Soybean stem and root rot, caused by Phytophthora sojae, is a destructive disease that significantly damages soybean production worldwide. C2H2 zinc finger protein (C2H2-ZFP) is a large transcription factor family in plants that plays crucial roles in stress response and hormone signal transduction. Given its importance, we analyzed the expression patterns of C2H2-ZFP family genes in response to P. sojae infection and selected four candidate genes to explore their molecular characteristics and functions related to P. sojae resistance. Subcellular localization analysis indicated that three ZFPs (GmZFP2, GmZFP3, and GmZFP4) were localized in the nucleus, while GmZFP1 was found in both the nucleus and plasma membrane. Dual-luciferase transient expression analysis revealed that all four ZFPs possessed transcriptional repression activation. Further transient expression in N. benthamiana leaves demonstrated that GmZFP2 induced significant cell death and reactive oxygen species (ROS) accumulation. GmZFP2 significantly enhanced the resistance to Phytophthora pathogens in N. benthamiana leaves and soybean hairy roots. This study provides insights in to the functional characterization of soybean ZFPs in Phytophthora resistance and demonstrates that GmZFP2 plays a positive role in P. sojae resistance in soybeans. |
| format | Article |
| id | doaj-art-1b0f1e84153d433ca2d064768eed612d |
| institution | OA Journals |
| issn | 1559-2316 1559-2324 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj-art-1b0f1e84153d433ca2d064768eed612d2025-08-20T01:51:30ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242025-12-0120110.1080/15592324.2025.24811852481185Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistanceYuting Chen0Xinyue Liu1Yanyan Zhou2Yu Zheng3Yating Xiao4Xingxing Yuan5Qiang Yan6Xin Chen7Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementSoybean (Glycine max) is one of the most important industrial and oilseed crops; however, the yield is threatened by the invasion of various pathogens. Soybean stem and root rot, caused by Phytophthora sojae, is a destructive disease that significantly damages soybean production worldwide. C2H2 zinc finger protein (C2H2-ZFP) is a large transcription factor family in plants that plays crucial roles in stress response and hormone signal transduction. Given its importance, we analyzed the expression patterns of C2H2-ZFP family genes in response to P. sojae infection and selected four candidate genes to explore their molecular characteristics and functions related to P. sojae resistance. Subcellular localization analysis indicated that three ZFPs (GmZFP2, GmZFP3, and GmZFP4) were localized in the nucleus, while GmZFP1 was found in both the nucleus and plasma membrane. Dual-luciferase transient expression analysis revealed that all four ZFPs possessed transcriptional repression activation. Further transient expression in N. benthamiana leaves demonstrated that GmZFP2 induced significant cell death and reactive oxygen species (ROS) accumulation. GmZFP2 significantly enhanced the resistance to Phytophthora pathogens in N. benthamiana leaves and soybean hairy roots. This study provides insights in to the functional characterization of soybean ZFPs in Phytophthora resistance and demonstrates that GmZFP2 plays a positive role in P. sojae resistance in soybeans.http://dx.doi.org/10.1080/15592324.2025.2481185soybeanphytophthora root rotc2h2 zinc finger protein (c2h2-zfp)gmzfp2transcriptional repression |
| spellingShingle | Yuting Chen Xinyue Liu Yanyan Zhou Yu Zheng Yating Xiao Xingxing Yuan Qiang Yan Xin Chen Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistance Plant Signaling & Behavior soybean phytophthora root rot c2h2 zinc finger protein (c2h2-zfp) gmzfp2 transcriptional repression |
| title | Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistance |
| title_full | Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistance |
| title_fullStr | Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistance |
| title_full_unstemmed | Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistance |
| title_short | Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistance |
| title_sort | functional characterization of four soybean c2h2 zinc finger genes in phytophthora resistance |
| topic | soybean phytophthora root rot c2h2 zinc finger protein (c2h2-zfp) gmzfp2 transcriptional repression |
| url | http://dx.doi.org/10.1080/15592324.2025.2481185 |
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