Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton
Sophorae Radix (Sophora flavescens Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Ra...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2018/6218430 |
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| author | Jeongyeo Lee Jaeeun Jung Seung-Hyun Son Hyun-Bi Kim Young-Hee Noh Sung Ran Min Kun-Hyang Park Dae-Soo Kim Sang Un Park Haeng-Soon Lee Cha Young Kim Hyun-Soon Kim Hyeong-Kyu Lee HyeRan Kim |
| author_facet | Jeongyeo Lee Jaeeun Jung Seung-Hyun Son Hyun-Bi Kim Young-Hee Noh Sung Ran Min Kun-Hyang Park Dae-Soo Kim Sang Un Park Haeng-Soon Lee Cha Young Kim Hyun-Soon Kim Hyeong-Kyu Lee HyeRan Kim |
| author_sort | Jeongyeo Lee |
| collection | DOAJ |
| description | Sophorae Radix (Sophora flavescens Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Radix were analyzed based on the qualitative HPLC analysis and evaluated in different organs and at different developmental stages. In total, 11 compounds were detected, and the composition of the roots and aerial parts (leaves, stems, and flowers) was significantly different. trans-Cinnamic acid and p-coumaric acid were observed only in the aerial parts. Large amounts of rutin and maackiain were detected in the roots. Four phenolic acid compounds (benzoic acid, caffeic acid, ferulic acid, and chlorogenic acid) and four flavonol compounds (kaempferol, catechin hydrate, epicatechin, and rutin) were higher in aerial parts than in roots. To identify putative genes involved in phenolic compounds biosynthesis, a total of 41 transcripts were investigated. Expression patterns of these selected genes, as well as the multiple isoforms for the genes, varied by organ and developmental stage, implying that they are involved in the biosynthesis of various phenolic compounds both spatially and temporally. |
| format | Article |
| id | doaj-art-1b0eb7c08b4a48668390c39e8c6d4c3e |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1b0eb7c08b4a48668390c39e8c6d4c3e2025-02-03T01:11:49ZengWileyThe Scientific World Journal2356-61401537-744X2018-01-01201810.1155/2018/62184306218430Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens AitonJeongyeo Lee0Jaeeun Jung1Seung-Hyun Son2Hyun-Bi Kim3Young-Hee Noh4Sung Ran Min5Kun-Hyang Park6Dae-Soo Kim7Sang Un Park8Haeng-Soon Lee9Cha Young Kim10Hyun-Soon Kim11Hyeong-Kyu Lee12HyeRan Kim13Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, Yeongudanji-ro 30, Ochang-eup, Cheongwon-gu, Cheongju-si 28116, Republic of KoreaKorea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaSophorae Radix (Sophora flavescens Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Radix were analyzed based on the qualitative HPLC analysis and evaluated in different organs and at different developmental stages. In total, 11 compounds were detected, and the composition of the roots and aerial parts (leaves, stems, and flowers) was significantly different. trans-Cinnamic acid and p-coumaric acid were observed only in the aerial parts. Large amounts of rutin and maackiain were detected in the roots. Four phenolic acid compounds (benzoic acid, caffeic acid, ferulic acid, and chlorogenic acid) and four flavonol compounds (kaempferol, catechin hydrate, epicatechin, and rutin) were higher in aerial parts than in roots. To identify putative genes involved in phenolic compounds biosynthesis, a total of 41 transcripts were investigated. Expression patterns of these selected genes, as well as the multiple isoforms for the genes, varied by organ and developmental stage, implying that they are involved in the biosynthesis of various phenolic compounds both spatially and temporally.http://dx.doi.org/10.1155/2018/6218430 |
| spellingShingle | Jeongyeo Lee Jaeeun Jung Seung-Hyun Son Hyun-Bi Kim Young-Hee Noh Sung Ran Min Kun-Hyang Park Dae-Soo Kim Sang Un Park Haeng-Soon Lee Cha Young Kim Hyun-Soon Kim Hyeong-Kyu Lee HyeRan Kim Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton The Scientific World Journal |
| title | Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton |
| title_full | Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton |
| title_fullStr | Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton |
| title_full_unstemmed | Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton |
| title_short | Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton |
| title_sort | profiling of the major phenolic compounds and their biosynthesis genes in sophora flavescens aiton |
| url | http://dx.doi.org/10.1155/2018/6218430 |
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