Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies
Many traditional Chinese medicines (TCMs) have been confirmed to have antibacterial activities. However, very few substances have been found to be active against Gram-negative bacteria. This study aimed to identify antimicrobial activity substances against Gram-negative bacteria from fourteen TCMs....
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2024/8963887 |
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| author | Meilan Liu Tingting Lin Liyuan Yao Hongfeng Chen Yu Lu Zhengguo Tao Haiquan Zhao Sheng-Xiang Qiu Liyun Zhao |
| author_facet | Meilan Liu Tingting Lin Liyuan Yao Hongfeng Chen Yu Lu Zhengguo Tao Haiquan Zhao Sheng-Xiang Qiu Liyun Zhao |
| author_sort | Meilan Liu |
| collection | DOAJ |
| description | Many traditional Chinese medicines (TCMs) have been confirmed to have antibacterial activities. However, very few substances have been found to be active against Gram-negative bacteria. This study aimed to identify antimicrobial activity substances against Gram-negative bacteria from fourteen TCMs. Fourteen TCMs with antibacterial potential were chosen for quantitative extraction and antibacterial activity assay, and the plant with the highest activity against Escherichia coli was selected to construct the component-target network. The following virtual screening and enzyme inhibition experiments were performed to analyse the antibacterial mechanisms of the compounds from Galla chinensis. The chemical constituents of Galla chinensis were identified by chemical fingerprinting. 1, 2, 3, 4, 6-Penta-O-galloyl-β-D-glucose (PGG) from Galla chinensis exhibited significant inhibition activity against adenylyl transferase (ATase) of E. coli and antibacterial activity against E. coli. Meanwhile, PGG was identified in the Galla chinensis ethanol extract as the abundant ingredient with a high content of 1.95% (w/w). PGG enriched in Galla chinensis is a promising natural antibiotic with the mode of action inhibiting ATase activity. To our knowledge, this is the first study attributing the antibacterial activity of PGG to its affinity with ATase. |
| format | Article |
| id | doaj-art-1b0d0ce657444054921802606ca33530 |
| institution | Kabale University |
| issn | 2090-9071 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-1b0d0ce657444054921802606ca335302025-02-03T10:24:54ZengWileyJournal of Chemistry2090-90712024-01-01202410.1155/2024/8963887Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based StudiesMeilan Liu0Tingting Lin1Liyuan Yao2Hongfeng Chen3Yu Lu4Zhengguo Tao5Haiquan Zhao6Sheng-Xiang Qiu7Liyun Zhao8State Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied BotanyState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied BotanyState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied BotanyState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied BotanyGuangzhou Leader Biotechnology Co., Ltd.Guangzhou Leader Biotechnology Co., Ltd.College of Life Science and EngineeringState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied BotanyState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied BotanyMany traditional Chinese medicines (TCMs) have been confirmed to have antibacterial activities. However, very few substances have been found to be active against Gram-negative bacteria. This study aimed to identify antimicrobial activity substances against Gram-negative bacteria from fourteen TCMs. Fourteen TCMs with antibacterial potential were chosen for quantitative extraction and antibacterial activity assay, and the plant with the highest activity against Escherichia coli was selected to construct the component-target network. The following virtual screening and enzyme inhibition experiments were performed to analyse the antibacterial mechanisms of the compounds from Galla chinensis. The chemical constituents of Galla chinensis were identified by chemical fingerprinting. 1, 2, 3, 4, 6-Penta-O-galloyl-β-D-glucose (PGG) from Galla chinensis exhibited significant inhibition activity against adenylyl transferase (ATase) of E. coli and antibacterial activity against E. coli. Meanwhile, PGG was identified in the Galla chinensis ethanol extract as the abundant ingredient with a high content of 1.95% (w/w). PGG enriched in Galla chinensis is a promising natural antibiotic with the mode of action inhibiting ATase activity. To our knowledge, this is the first study attributing the antibacterial activity of PGG to its affinity with ATase.http://dx.doi.org/10.1155/2024/8963887 |
| spellingShingle | Meilan Liu Tingting Lin Liyuan Yao Hongfeng Chen Yu Lu Zhengguo Tao Haiquan Zhao Sheng-Xiang Qiu Liyun Zhao Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies Journal of Chemistry |
| title | Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies |
| title_full | Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies |
| title_fullStr | Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies |
| title_full_unstemmed | Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies |
| title_short | Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies |
| title_sort | antibacterial mechanisms of constituents from galla chinensis revealed by experimental and virtual screening based studies |
| url | http://dx.doi.org/10.1155/2024/8963887 |
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