Opa-interacting protein 5 modulates docetaxel-induced cell death via regulation of mitophagy in gastric cancer
Damage to mitochondria induces mitophagy, a cellular process that is gaining interest for its therapeutic relevance to a variety of human diseases. However, the mechanism underlying mitochondrial depolarization and clearance in mitophagy remains poorly understood. We previously reported that mitocho...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2017-10-01
|
| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317733985 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849709488054992896 |
|---|---|
| author | Tae Woo Kim Seon-Jin Lee Young-Jun Park Sang Yoon Park Byung Moo Oh Yun Sun Park Bo-Yeon Kim Young-Ha Lee Hee Jun Cho Suk Ran Yoon Yong-Kyung Choe Hee Gu Lee |
| author_facet | Tae Woo Kim Seon-Jin Lee Young-Jun Park Sang Yoon Park Byung Moo Oh Yun Sun Park Bo-Yeon Kim Young-Ha Lee Hee Jun Cho Suk Ran Yoon Yong-Kyung Choe Hee Gu Lee |
| author_sort | Tae Woo Kim |
| collection | DOAJ |
| description | Damage to mitochondria induces mitophagy, a cellular process that is gaining interest for its therapeutic relevance to a variety of human diseases. However, the mechanism underlying mitochondrial depolarization and clearance in mitophagy remains poorly understood. We previously reported that mitochondria-induced cell death was caused by knockdown of Neisseria gonorrhoeae opacity-associated-interacting protein 5 in gastric cancer. In this study, we show that Neisseria gonorrhoeae opacity-associated-interacting protein 5 loss and gain of function modulates mitophagy induced by treatment with docetaxel, a chemotherapy drug for gastric cancer. The activation of mitophagy by Neisseria gonorrhoeae opacity-associated-interacting protein 5 overexpression promoted cell survival, preventing docetaxel-induced mitochondrial clearance. Conversely, short interfering RNA–mediated knockdown of Neisseria gonorrhoeae opacity-associated-interacting protein 5 accelerated docetaxel-induced apoptosis while increasing mitochondrial depolarization, reactive oxygen species, and endoplasmic reticulum stress and decreasing adenosine triphosphate production. We also found that the mitochondrial outer membrane proteins mitofusin 2 and phosphatase and tensin homolog–induced putative kinase 1 colocalized with Neisseria gonorrhoeae opacity-associated-interacting protein 5 in mitochondria and that mitofusin 2 knockdown altered Neisseria gonorrhoeae opacity-associated-interacting protein 5 expression. These findings indicate that Neisseria gonorrhoeae opacity-associated-interacting protein 5 modulates docetaxel-induced mitophagic cell death and therefore suggest that this protein comprises a potential therapeutic target for gastric cancer treatment. |
| format | Article |
| id | doaj-art-1b05d75414ae4bb996b725c1795deda7 |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-1b05d75414ae4bb996b725c1795deda72025-08-20T03:15:16ZengSAGE PublishingTumor Biology1423-03802017-10-013910.1177/1010428317733985Opa-interacting protein 5 modulates docetaxel-induced cell death via regulation of mitophagy in gastric cancerTae Woo Kim0Seon-Jin Lee1Young-Jun Park2Sang Yoon Park3Byung Moo Oh4Yun Sun Park5Bo-Yeon Kim6Young-Ha Lee7Hee Jun Cho8Suk Ran Yoon9Yong-Kyung Choe10Hee Gu Lee11Department of Biomolecular Science, University of Science & Technology (UST), Daejeon, Republic of KoreaDepartment of Biomolecular Science, University of Science & Technology (UST), Daejeon, Republic of KoreaDepartment of Biomolecular Science, University of Science & Technology (UST), Daejeon, Republic of KoreaDepartment of Biochemistry, College of Natural Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Biomolecular Science, University of Science & Technology (UST), Daejeon, Republic of KoreaImmunotherapy Convergence Research Group, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of KoreaWorld Class Institute, Korea Research Institute of Bioscience & Biotechnology, Cheongju, Republic of KoreaDepartment of Infection Biology, School of Medicine, Chungnam National University, Daejeon, Republic of KoreaImmunotherapy Convergence Research Group, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of KoreaImmunotherapy Convergence Research Group, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of KoreaImmunotherapy Convergence Research Group, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of KoreaDepartment of Biomolecular Science, University of Science & Technology (UST), Daejeon, Republic of KoreaDamage to mitochondria induces mitophagy, a cellular process that is gaining interest for its therapeutic relevance to a variety of human diseases. However, the mechanism underlying mitochondrial depolarization and clearance in mitophagy remains poorly understood. We previously reported that mitochondria-induced cell death was caused by knockdown of Neisseria gonorrhoeae opacity-associated-interacting protein 5 in gastric cancer. In this study, we show that Neisseria gonorrhoeae opacity-associated-interacting protein 5 loss and gain of function modulates mitophagy induced by treatment with docetaxel, a chemotherapy drug for gastric cancer. The activation of mitophagy by Neisseria gonorrhoeae opacity-associated-interacting protein 5 overexpression promoted cell survival, preventing docetaxel-induced mitochondrial clearance. Conversely, short interfering RNA–mediated knockdown of Neisseria gonorrhoeae opacity-associated-interacting protein 5 accelerated docetaxel-induced apoptosis while increasing mitochondrial depolarization, reactive oxygen species, and endoplasmic reticulum stress and decreasing adenosine triphosphate production. We also found that the mitochondrial outer membrane proteins mitofusin 2 and phosphatase and tensin homolog–induced putative kinase 1 colocalized with Neisseria gonorrhoeae opacity-associated-interacting protein 5 in mitochondria and that mitofusin 2 knockdown altered Neisseria gonorrhoeae opacity-associated-interacting protein 5 expression. These findings indicate that Neisseria gonorrhoeae opacity-associated-interacting protein 5 modulates docetaxel-induced mitophagic cell death and therefore suggest that this protein comprises a potential therapeutic target for gastric cancer treatment.https://doi.org/10.1177/1010428317733985 |
| spellingShingle | Tae Woo Kim Seon-Jin Lee Young-Jun Park Sang Yoon Park Byung Moo Oh Yun Sun Park Bo-Yeon Kim Young-Ha Lee Hee Jun Cho Suk Ran Yoon Yong-Kyung Choe Hee Gu Lee Opa-interacting protein 5 modulates docetaxel-induced cell death via regulation of mitophagy in gastric cancer Tumor Biology |
| title | Opa-interacting protein 5 modulates docetaxel-induced cell death via regulation of mitophagy in gastric cancer |
| title_full | Opa-interacting protein 5 modulates docetaxel-induced cell death via regulation of mitophagy in gastric cancer |
| title_fullStr | Opa-interacting protein 5 modulates docetaxel-induced cell death via regulation of mitophagy in gastric cancer |
| title_full_unstemmed | Opa-interacting protein 5 modulates docetaxel-induced cell death via regulation of mitophagy in gastric cancer |
| title_short | Opa-interacting protein 5 modulates docetaxel-induced cell death via regulation of mitophagy in gastric cancer |
| title_sort | opa interacting protein 5 modulates docetaxel induced cell death via regulation of mitophagy in gastric cancer |
| url | https://doi.org/10.1177/1010428317733985 |
| work_keys_str_mv | AT taewookim opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT seonjinlee opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT youngjunpark opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT sangyoonpark opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT byungmoooh opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT yunsunpark opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT boyeonkim opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT younghalee opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT heejuncho opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT sukranyoon opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT yongkyungchoe opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer AT heegulee opainteractingprotein5modulatesdocetaxelinducedcelldeathviaregulationofmitophagyingastriccancer |