Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels
Background: In topical preparations, four herbal compounds, namely mangiferin (MF), resveratrol (RV), silybin (SB), and curcumin (CR) need to be quantified quickly and accurately. Objective: This study utilizes a Franz diffusion cell setup to evaluate the suitability of reversed-phase ultra-fast liq...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2024-10-01
|
| Series: | Pharmaceutical and Biomedical Research |
| Subjects: | |
| Online Access: | http://pbr.mazums.ac.ir/article-1-603-en.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850181291085922304 |
|---|---|
| author | Bajee Shaik Biswajit Swain Eswar Gupta Maddi Srinivas Maddi |
| author_facet | Bajee Shaik Biswajit Swain Eswar Gupta Maddi Srinivas Maddi |
| author_sort | Bajee Shaik |
| collection | DOAJ |
| description | Background: In topical preparations, four herbal compounds, namely mangiferin (MF),
resveratrol (RV), silybin (SB), and curcumin (CR) need to be quantified quickly and accurately.
Objective: This study utilizes a Franz diffusion cell setup to evaluate the suitability of reversed-phase ultra-fast liquid chromatography (UFLC) for assessing MF, RV, SB, and CR in a mouse skin.
Methods: A C-18 column (250×4.6 mm, 5 µm) was used at 40 °C for chromatographic separation. The mobile phase, a gradient of acetonitrile and 0.1% formic acid in high-performance liquid chromatography (HPLC) grade water was pumped at a rate of 1 mL per min. A 20 µL sample was loaded, and analytes were accessed using a photodiode array (PDA) detector. The method validation included selectivity, linearity (2-20 µg/mL), accuracy, precision, robustness, and sensitivity. The method’s run time was 20 min.
Results: The UFLC method was selective for the given compounds, and over the concentration range of 2–20 µg/mL, the method’s linearity was demonstrated with a correlation coefficient >0.99. Additionally, it was accurate, precise, robust, and sensitive, with a total run time of 20 min.
Conclusion: The validated reversed-phase UFLC method meets the highlighted need for accurate and quick measurement of MF, RV, SB, and CR in topical formulations. Its effective validation and application in researching drug features using a Franz diffusion cell apparatus make it an important analytical tool in pharmaceutical research, ensuring precise drug content, homogeneity, and release properties in topical formulations. |
| format | Article |
| id | doaj-art-1b0430c552f44dda9f58896fdcac6018 |
| institution | OA Journals |
| issn | 2423-4494 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical and Biomedical Research |
| spelling | doaj-art-1b0430c552f44dda9f58896fdcac60182025-08-20T02:17:57ZengMazandaran University of Medical SciencesPharmaceutical and Biomedical Research2423-44942024-10-01104303318Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical HydrogelsBajee Shaik0Biswajit Swain1Eswar Gupta Maddi2Srinivas Maddi3 Formulation and Analytical Research and Development Division, Acubiosys Private Limited, Hyderabad, India. Formulation and Analytical Research and Development Division, Acubiosys Private Limited, Hyderabad, India. Department of Biopharmaceutics and Pharmaceutical Technology, Krupanidhi College of Pharmacy, Bangalore, India. Formulation and Analytical Research and Development Division, Acubiosys Private Limited, Hyderabad, India. Background: In topical preparations, four herbal compounds, namely mangiferin (MF), resveratrol (RV), silybin (SB), and curcumin (CR) need to be quantified quickly and accurately. Objective: This study utilizes a Franz diffusion cell setup to evaluate the suitability of reversed-phase ultra-fast liquid chromatography (UFLC) for assessing MF, RV, SB, and CR in a mouse skin. Methods: A C-18 column (250×4.6 mm, 5 µm) was used at 40 °C for chromatographic separation. The mobile phase, a gradient of acetonitrile and 0.1% formic acid in high-performance liquid chromatography (HPLC) grade water was pumped at a rate of 1 mL per min. A 20 µL sample was loaded, and analytes were accessed using a photodiode array (PDA) detector. The method validation included selectivity, linearity (2-20 µg/mL), accuracy, precision, robustness, and sensitivity. The method’s run time was 20 min. Results: The UFLC method was selective for the given compounds, and over the concentration range of 2–20 µg/mL, the method’s linearity was demonstrated with a correlation coefficient >0.99. Additionally, it was accurate, precise, robust, and sensitive, with a total run time of 20 min. Conclusion: The validated reversed-phase UFLC method meets the highlighted need for accurate and quick measurement of MF, RV, SB, and CR in topical formulations. Its effective validation and application in researching drug features using a Franz diffusion cell apparatus make it an important analytical tool in pharmaceutical research, ensuring precise drug content, homogeneity, and release properties in topical formulations.http://pbr.mazums.ac.ir/article-1-603-en.pdfdrug releasepsoriasisliquid chromatographhydrogelsherbals |
| spellingShingle | Bajee Shaik Biswajit Swain Eswar Gupta Maddi Srinivas Maddi Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels Pharmaceutical and Biomedical Research drug release psoriasis liquid chromatograph hydrogels herbals |
| title | Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels |
| title_full | Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels |
| title_fullStr | Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels |
| title_full_unstemmed | Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels |
| title_short | Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels |
| title_sort | novel rp uflc methodology for concurrent assessment of curcumin resveratrol silybin and mangiferin from topical hydrogels |
| topic | drug release psoriasis liquid chromatograph hydrogels herbals |
| url | http://pbr.mazums.ac.ir/article-1-603-en.pdf |
| work_keys_str_mv | AT bajeeshaik novelrpuflcmethodologyforconcurrentassessmentofcurcuminresveratrolsilybinandmangiferinfromtopicalhydrogels AT biswajitswain novelrpuflcmethodologyforconcurrentassessmentofcurcuminresveratrolsilybinandmangiferinfromtopicalhydrogels AT eswarguptamaddi novelrpuflcmethodologyforconcurrentassessmentofcurcuminresveratrolsilybinandmangiferinfromtopicalhydrogels AT srinivasmaddi novelrpuflcmethodologyforconcurrentassessmentofcurcuminresveratrolsilybinandmangiferinfromtopicalhydrogels |