A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells
The possibility of tightly controlling the cellular microenvironment within microfluidic devices represents an important step toward precision analysis of cellular phenotypes in vitro. Microfluidic platforms that allow both long-term mammalian cell culture and dynamic modulation of the culture envir...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/7/427 |
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| author | Adil Mustafa Antonella La Regina Elisa Pedone Ahmet Erten Lucia Marucci |
| author_facet | Adil Mustafa Antonella La Regina Elisa Pedone Ahmet Erten Lucia Marucci |
| author_sort | Adil Mustafa |
| collection | DOAJ |
| description | The possibility of tightly controlling the cellular microenvironment within microfluidic devices represents an important step toward precision analysis of cellular phenotypes in vitro. Microfluidic platforms that allow both long-term mammalian cell culture and dynamic modulation of the culture environment can support quantitative studies of cells’ responses to drugs. Here, we report the design and testing of a novel microfluidic device of simple production (single Polydimethylsiloxane layer), which integrates a micromixer with vacuum-assisted cell loading for long-term mammalian cell culture and dynamic mixing of four different culture media. Finite element modeling was used to predict flow rates and device dimensions to achieve diffusion-based fluid mixing. The device showed efficient mixing and dynamic exchange of media in the cell-trapping chambers, and viability of mammalian cells cultured for long-term in the device. This work represents the first attempt to integrate single-layer microfluidic mixing devices with vacuum-assisted cell-loading systems for mammalian cell culture and dynamic stimulation. |
| format | Article |
| id | doaj-art-1b042eacf05c431f809c59bb340e91b1 |
| institution | DOAJ |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-1b042eacf05c431f809c59bb340e91b12025-08-20T02:45:33ZengMDPI AGBiosensors2079-63742025-07-0115742710.3390/bios15070427A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian CellsAdil Mustafa0Antonella La Regina1Elisa Pedone2Ahmet Erten3Lucia Marucci4School of Engineering Mathematics and Technology, University of Bristol, Bristol BS8 1TW, UKSchool of Engineering Mathematics and Technology, University of Bristol, Bristol BS8 1TW, UKSchool of Engineering Mathematics and Technology, University of Bristol, Bristol BS8 1TW, UKDepartment of Electronics and Communication Engineering, Istanbul Technical University, Istanbul 34469, TurkeySchool of Engineering Mathematics and Technology, University of Bristol, Bristol BS8 1TW, UKThe possibility of tightly controlling the cellular microenvironment within microfluidic devices represents an important step toward precision analysis of cellular phenotypes in vitro. Microfluidic platforms that allow both long-term mammalian cell culture and dynamic modulation of the culture environment can support quantitative studies of cells’ responses to drugs. Here, we report the design and testing of a novel microfluidic device of simple production (single Polydimethylsiloxane layer), which integrates a micromixer with vacuum-assisted cell loading for long-term mammalian cell culture and dynamic mixing of four different culture media. Finite element modeling was used to predict flow rates and device dimensions to achieve diffusion-based fluid mixing. The device showed efficient mixing and dynamic exchange of media in the cell-trapping chambers, and viability of mammalian cells cultured for long-term in the device. This work represents the first attempt to integrate single-layer microfluidic mixing devices with vacuum-assisted cell-loading systems for mammalian cell culture and dynamic stimulation.https://www.mdpi.com/2079-6374/15/7/427microfluidicsmicromixersmammalian cellssingle layerPDMS |
| spellingShingle | Adil Mustafa Antonella La Regina Elisa Pedone Ahmet Erten Lucia Marucci A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells Biosensors microfluidics micromixers mammalian cells single layer PDMS |
| title | A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells |
| title_full | A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells |
| title_fullStr | A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells |
| title_full_unstemmed | A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells |
| title_short | A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells |
| title_sort | novel single layer microfluidic device for dynamic stimulation culture and imaging of mammalian cells |
| topic | microfluidics micromixers mammalian cells single layer PDMS |
| url | https://www.mdpi.com/2079-6374/15/7/427 |
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