Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China
BackgroundBovine hepacivirus (BovHepV) is a recently identified member of the expanding genus Hepacivirus within the family Flaviviridae. However, the genetic diversity, geographical distribution, and host range of the virus remains poorly understood.MethodsIn this study, serum samples from cattle a...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| author | Jingge Ma Jingge Ma Zhiwei Wei Liang Li Wei Wang Ziyan Liu Ziyan Liu Ning Liu Feng Wei Xiangyu Zheng Zedong Wang |
| author_facet | Jingge Ma Jingge Ma Zhiwei Wei Liang Li Wei Wang Ziyan Liu Ziyan Liu Ning Liu Feng Wei Xiangyu Zheng Zedong Wang |
| author_sort | Jingge Ma |
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| description | BackgroundBovine hepacivirus (BovHepV) is a recently identified member of the expanding genus Hepacivirus within the family Flaviviridae. However, the genetic diversity, geographical distribution, and host range of the virus remains poorly understood.MethodsIn this study, serum samples from cattle and sheep were obtained in Hulunbuir and pooled to establish RNA libraries, which were then analyzed using transcriptome sequencing. BovHepV-positive samples were confirmed using semi-nested PCR with primers designed based on the obtained viral sequences. Comprehensive bioinformatics analyses were employed to assess sequence similarity, phylogenetic evolution, and recombination of the obtained viral strains.ResultsA total of 988 serum samples from sheep (520) and cattle (468) were collected from 12 administrative districts in Hulunbuir from June to August, 2022. Semi-nested PCR revealed 6 BovHepV-positive districts with prevalence ranging from 2.0% to 35.0% in cattle, and one BovHepV-positive district with prevalence of 2.5% in sheep. The nucleotide sequence identities between viral strains from sheep and cattle ranged from 91.3% to 93.8%, while the amino acid sequence identities were between 95.4% and 96.7%. Phylogenetic analyses classified the obtained BovHepV strains within subtype G, genotype 1. Recombination analysis revealed the intergenerational relationships among the viral strains obtained from cattle and sheep.ConclusionWe identified genetic diversity in subtype G strains in cattle and detected a BovHepV strain in a sheep for the first time in northeastern China, confirming cross-species transmission and co-circulation between cattle and sheep, thus expanding the virus’s host range. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-1afae4ac6a0c429698a6460d4cc2599a2025-01-28T09:10:56ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011510.3389/fcimb.2025.15408491540849Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern ChinaJingge Ma0Jingge Ma1Zhiwei Wei2Liang Li3Wei Wang4Ziyan Liu5Ziyan Liu6Ning Liu7Feng Wei8Xiangyu Zheng9Zedong Wang10Department of Infectious Diseases, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, Jilin, ChinaLaboratory of Pathogen Microbiology and Immunology, College of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaDepartment of Neurology and Neuroscience Center, The First Hospital of Jilin University, Changchun, Jilin, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaHulunbuir Animal Disease Control Center, Hailar, Inner Mongolia Autonomous Region, ChinaDepartment of Infectious Diseases, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, Jilin, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaDepartment of Infectious Diseases, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, Jilin, ChinaLaboratory of Pathogen Microbiology and Immunology, College of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaDepartment of Neurology and Neuroscience Center, The First Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Infectious Diseases, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, Jilin, ChinaBackgroundBovine hepacivirus (BovHepV) is a recently identified member of the expanding genus Hepacivirus within the family Flaviviridae. However, the genetic diversity, geographical distribution, and host range of the virus remains poorly understood.MethodsIn this study, serum samples from cattle and sheep were obtained in Hulunbuir and pooled to establish RNA libraries, which were then analyzed using transcriptome sequencing. BovHepV-positive samples were confirmed using semi-nested PCR with primers designed based on the obtained viral sequences. Comprehensive bioinformatics analyses were employed to assess sequence similarity, phylogenetic evolution, and recombination of the obtained viral strains.ResultsA total of 988 serum samples from sheep (520) and cattle (468) were collected from 12 administrative districts in Hulunbuir from June to August, 2022. Semi-nested PCR revealed 6 BovHepV-positive districts with prevalence ranging from 2.0% to 35.0% in cattle, and one BovHepV-positive district with prevalence of 2.5% in sheep. The nucleotide sequence identities between viral strains from sheep and cattle ranged from 91.3% to 93.8%, while the amino acid sequence identities were between 95.4% and 96.7%. Phylogenetic analyses classified the obtained BovHepV strains within subtype G, genotype 1. Recombination analysis revealed the intergenerational relationships among the viral strains obtained from cattle and sheep.ConclusionWe identified genetic diversity in subtype G strains in cattle and detected a BovHepV strain in a sheep for the first time in northeastern China, confirming cross-species transmission and co-circulation between cattle and sheep, thus expanding the virus’s host range.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1540849/fullbovine hepacivirus (BovHepV)sheepcattlephylogenetic evolutionChina |
| spellingShingle | Jingge Ma Jingge Ma Zhiwei Wei Liang Li Wei Wang Ziyan Liu Ziyan Liu Ning Liu Feng Wei Xiangyu Zheng Zedong Wang Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China Frontiers in Cellular and Infection Microbiology bovine hepacivirus (BovHepV) sheep cattle phylogenetic evolution China |
| title | Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China |
| title_full | Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China |
| title_fullStr | Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China |
| title_full_unstemmed | Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China |
| title_short | Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China |
| title_sort | detection and characterization of bovine hepacivirus in cattle and sheep from hulunbuir northeastern china |
| topic | bovine hepacivirus (BovHepV) sheep cattle phylogenetic evolution China |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1540849/full |
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